African swine fever virus plasmid library: An invaluable resource for research
African swine fever virus (ASFV) is a large, complex DNA virus from the family <i>Asfarviridae</i> responsible for African swine fever (ASF), a highly fatal disease affecting domestic and wild pig populations with mortality rates reaching up to 100%. The disease, originally endemic in Africa and Sar...
| Main Authors: | , , , |
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| Format: | Informe técnico |
| Language: | Inglés |
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International Livestock Research Institute
2024
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| Online Access: | https://hdl.handle.net/10568/169824 |
| _version_ | 1855513308858155008 |
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| author | Machuka, Eunice M. Gachogo, Rachael Rodriguez, F. Lacasta, Anna |
| author_browse | Gachogo, Rachael Lacasta, Anna Machuka, Eunice M. Rodriguez, F. |
| author_facet | Machuka, Eunice M. Gachogo, Rachael Rodriguez, F. Lacasta, Anna |
| author_sort | Machuka, Eunice M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African swine fever virus (ASFV) is a large, complex DNA virus from the family <i>Asfarviridae</i> responsible for African swine fever (ASF), a highly fatal disease affecting domestic and wild pig populations with mortality rates reaching up to 100%. The disease, originally endemic in Africa and Sardinia, has spread globally, leading to significant economic losses. Despite extensive research, no effective commercial vaccine for ASFV is available globally, driving continued efforts to understand the genetic and functional properties of the virus. Plasmids, small circular DNA molecules, play a crucial role in research by enabling cloning, gene expression, and protein production. This report presents the development of an ASFV plasmid library and a corresponding database to support research efforts in ASF vaccine development, gene function analysis, and protein characterization. The library contains plasmids encoding 161 ASFV open reading frames (ORFs) cloned under the control of a CMV promoter. The plasmid collection facilitates antigen screening and functional assays with restriction enzyme sites within the plasmids that can be leveraged for cloning and expression studies, ensuring the versatility and reproducibility of the ASFV research. The development of this plasmid library is a crucial resource for accelerating ASF vaccine research and advancing molecular studies. It also establishes a foundation for its application to other microorganisms, enhancing its utility in broader infectious disease research. |
| format | Informe técnico |
| id | CGSpace169824 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Livestock Research Institute |
| publisherStr | International Livestock Research Institute |
| record_format | dspace |
| spelling | CGSpace1698242025-12-02T10:59:51Z African swine fever virus plasmid library: An invaluable resource for research Machuka, Eunice M. Gachogo, Rachael Rodriguez, F. Lacasta, Anna african swine fever research African swine fever virus (ASFV) is a large, complex DNA virus from the family <i>Asfarviridae</i> responsible for African swine fever (ASF), a highly fatal disease affecting domestic and wild pig populations with mortality rates reaching up to 100%. The disease, originally endemic in Africa and Sardinia, has spread globally, leading to significant economic losses. Despite extensive research, no effective commercial vaccine for ASFV is available globally, driving continued efforts to understand the genetic and functional properties of the virus. Plasmids, small circular DNA molecules, play a crucial role in research by enabling cloning, gene expression, and protein production. This report presents the development of an ASFV plasmid library and a corresponding database to support research efforts in ASF vaccine development, gene function analysis, and protein characterization. The library contains plasmids encoding 161 ASFV open reading frames (ORFs) cloned under the control of a CMV promoter. The plasmid collection facilitates antigen screening and functional assays with restriction enzyme sites within the plasmids that can be leveraged for cloning and expression studies, ensuring the versatility and reproducibility of the ASFV research. The development of this plasmid library is a crucial resource for accelerating ASF vaccine research and advancing molecular studies. It also establishes a foundation for its application to other microorganisms, enhancing its utility in broader infectious disease research. 2024-10 2025-01-24T08:17:34Z 2025-01-24T08:17:34Z Report https://hdl.handle.net/10568/169824 en Open Access application/pdf International Livestock Research Institute Machuka, E.M., Gachogo, R., Rodriguez, F. and Lacasta, A. 2024. African swine fever virus plasmid library: an invaluable resource for research. Nairobi, Kenya: ILRI. |
| spellingShingle | african swine fever research Machuka, Eunice M. Gachogo, Rachael Rodriguez, F. Lacasta, Anna African swine fever virus plasmid library: An invaluable resource for research |
| title | African swine fever virus plasmid library: An invaluable resource for research |
| title_full | African swine fever virus plasmid library: An invaluable resource for research |
| title_fullStr | African swine fever virus plasmid library: An invaluable resource for research |
| title_full_unstemmed | African swine fever virus plasmid library: An invaluable resource for research |
| title_short | African swine fever virus plasmid library: An invaluable resource for research |
| title_sort | african swine fever virus plasmid library an invaluable resource for research |
| topic | african swine fever research |
| url | https://hdl.handle.net/10568/169824 |
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