African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries
African swine fever (ASF) is a highly fatal disease of pigs. It is a threat to the pig industry as it lowers production and significantly impacts on livelihoods of small-scale producers, while outbreaks continue to be reported in Africa, Europe, Asia, and more recently in the Caribbean. There are no...
| Autores principales: | , , |
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| Formato: | Informe técnico |
| Lenguaje: | Inglés |
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International Livestock Research Institute
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/169321 |
| _version_ | 1855542953416589312 |
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| author | Lacasta, Anna Oba, Peter Dione, Michel M. |
| author_browse | Dione, Michel M. Lacasta, Anna Oba, Peter |
| author_facet | Lacasta, Anna Oba, Peter Dione, Michel M. |
| author_sort | Lacasta, Anna |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African swine fever (ASF) is a highly fatal disease of pigs. It is a threat to the pig industry as it lowers production and significantly impacts on livelihoods of small-scale producers, while outbreaks continue to be reported in Africa, Europe, Asia, and more recently in the Caribbean. There are no globally available treatment nor commercial vaccines against ASF. The most effective measures to control the disease include early detection using rapid diagnostic test and separation of infected animals from healthy ones, usually by culling/disposing affected pigs.
Diagnostics of ASF range from clinical diagnostics to the identification of the agent and serological tests, according to the World Organisation for Animal Health. Clinical diagnostics are often challenging since ASF shares common clinical signs with other pig diseases (e.g. classical swine fever) in its acute form; and for the chronic forms subtle clinical signs can be overlooked. The lesion identification has the limitation of post-mortem diagnostics and not possible to implement in non-death pigs.
Laboratory diagnostics are more accurate, including the identification of the viral particle by means of isolation of the virus from animal samples, hemadsorption tests, fluorescent antibody test; or the detection of the viral genome by polymerase chain reaction.
Serological tests are also useful to detect previous exposure to the virus. They include enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test and immunoblotting.
However, even if accurate, all these laboratory tests pose the same problem; they are difficult to implement in the field where infrastructures and human expertise are limited or non-existent, as it is the case in countries where the disease is endemic. The time to get results is also key for the control of African swine fever virus (ASFV) in the field and all the mentioned tests do not provide immediate results. |
| format | Informe técnico |
| id | CGSpace169321 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Livestock Research Institute |
| publisherStr | International Livestock Research Institute |
| record_format | dspace |
| spelling | CGSpace1693212025-11-04T20:20:18Z African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries Lacasta, Anna Oba, Peter Dione, Michel M. african swine fever diagnosis swine African swine fever (ASF) is a highly fatal disease of pigs. It is a threat to the pig industry as it lowers production and significantly impacts on livelihoods of small-scale producers, while outbreaks continue to be reported in Africa, Europe, Asia, and more recently in the Caribbean. There are no globally available treatment nor commercial vaccines against ASF. The most effective measures to control the disease include early detection using rapid diagnostic test and separation of infected animals from healthy ones, usually by culling/disposing affected pigs. Diagnostics of ASF range from clinical diagnostics to the identification of the agent and serological tests, according to the World Organisation for Animal Health. Clinical diagnostics are often challenging since ASF shares common clinical signs with other pig diseases (e.g. classical swine fever) in its acute form; and for the chronic forms subtle clinical signs can be overlooked. The lesion identification has the limitation of post-mortem diagnostics and not possible to implement in non-death pigs. Laboratory diagnostics are more accurate, including the identification of the viral particle by means of isolation of the virus from animal samples, hemadsorption tests, fluorescent antibody test; or the detection of the viral genome by polymerase chain reaction. Serological tests are also useful to detect previous exposure to the virus. They include enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test and immunoblotting. However, even if accurate, all these laboratory tests pose the same problem; they are difficult to implement in the field where infrastructures and human expertise are limited or non-existent, as it is the case in countries where the disease is endemic. The time to get results is also key for the control of African swine fever virus (ASFV) in the field and all the mentioned tests do not provide immediate results. 2024-12-30 2025-01-17T12:08:22Z 2025-01-17T12:08:22Z Report https://hdl.handle.net/10568/169321 en Open Access application/pdf International Livestock Research Institute Lacasta, A., Oba, P. and Dione, M. 2024. African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries. Nairobi, Kenya: ILRI. |
| spellingShingle | african swine fever diagnosis swine Lacasta, Anna Oba, Peter Dione, Michel M. African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title | African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title_full | African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title_fullStr | African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title_full_unstemmed | African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title_short | African swine fever virus antibody rapid detection assays evaluation: Evaluation of rapid lateral flow assays for the detection of antibodies against African swine fever virus for their implementation in low- and middle-income countries |
| title_sort | african swine fever virus antibody rapid detection assays evaluation evaluation of rapid lateral flow assays for the detection of antibodies against african swine fever virus for their implementation in low and middle income countries |
| topic | african swine fever diagnosis swine |
| url | https://hdl.handle.net/10568/169321 |
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