The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya
Background: African buffalo (<i>Syncerus caffer</i>) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include a range of viruses and pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations of mammalian blood as a sterile en...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
BioMed Central
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/169188 |
| _version_ | 1855520915882770432 |
|---|---|
| author | Nyamota, Richard Middlebrook, E.A. Abkallo, Hussein M. Akoko, James M. Gakuya, F. Wambua, Lillian Ronoh, B. Lekolool, I. Mwatondo, Athman Muturi, Mathew Bett, Bernard K. Fair, J.M. Bartlow, A.W. |
| author_browse | Abkallo, Hussein M. Akoko, James M. Bartlow, A.W. Bett, Bernard K. Fair, J.M. Gakuya, F. Lekolool, I. Middlebrook, E.A. Muturi, Mathew Mwatondo, Athman Nyamota, Richard Ronoh, B. Wambua, Lillian |
| author_facet | Nyamota, Richard Middlebrook, E.A. Abkallo, Hussein M. Akoko, James M. Gakuya, F. Wambua, Lillian Ronoh, B. Lekolool, I. Mwatondo, Athman Muturi, Mathew Bett, Bernard K. Fair, J.M. Bartlow, A.W. |
| author_sort | Nyamota, Richard |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: African buffalo (<i>Syncerus caffer</i>) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include a range of viruses and pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations of mammalian blood as a sterile environment, blood microbiome sequencing could become crucial for agnostic biosurveillance. This study investigated the blood microbiome of clinically healthy wild buffaloes in Kenya to determine its applicability in agnostic testing for bacteria in apparently healthy wild animals.
Methods: Whole blood and serum samples were collected from 46 wild African buffalos from Meru National Park (30), Buffalo Springs (6) and Shaba (10) National Reserves in upper eastern Kenya. Total deoxyribonucleic acid (DNA) was extracted from these samples and subjected to amplicon-based sequencing targeting the 16 S rRNA gene. The bacteria operational taxonomic units (OTU) were identified to species levels by mapping the generated V12 and V45 regions of 16 S rRNA gene to the SILVA database. These OTU tables were used to infer the microbial abundance in each sample type and at the individual animal level. The sequences for the corresponding OTUs were also used to generate phylogenetic trees and thus infer evolution for the OTUs of interest.
Results: Here, we demonstrate that buffaloes harbor many bacteria in their blood. We also report a diversity of 16 S rRNA gene sequences for <i>Anaplasma</i> and <i>Mycoplasma</i> from individual animals. By sequencing both whole blood and serum in triplicate for each animal, we provide evidence of the differences in detecting bacteria in both sample types.
Conclusions: Diverse bacteria, including some potential pathogens, can be found in the blood of clinically healthy wild African buffalo. Agnostic surveillance for such pathogens can be achieved through blood microbiome sequencing. However, considerations for the question being asked for the blood microbiome in wildlife will impact the choice for using whole blood or serum for sequencing. |
| format | Journal Article |
| id | CGSpace169188 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | CGSpace1691882025-12-08T09:54:28Z The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya Nyamota, Richard Middlebrook, E.A. Abkallo, Hussein M. Akoko, James M. Gakuya, F. Wambua, Lillian Ronoh, B. Lekolool, I. Mwatondo, Athman Muturi, Mathew Bett, Bernard K. Fair, J.M. Bartlow, A.W. african buffaloes diagnosis microbiomes Background: African buffalo (<i>Syncerus caffer</i>) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include a range of viruses and pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations of mammalian blood as a sterile environment, blood microbiome sequencing could become crucial for agnostic biosurveillance. This study investigated the blood microbiome of clinically healthy wild buffaloes in Kenya to determine its applicability in agnostic testing for bacteria in apparently healthy wild animals. Methods: Whole blood and serum samples were collected from 46 wild African buffalos from Meru National Park (30), Buffalo Springs (6) and Shaba (10) National Reserves in upper eastern Kenya. Total deoxyribonucleic acid (DNA) was extracted from these samples and subjected to amplicon-based sequencing targeting the 16 S rRNA gene. The bacteria operational taxonomic units (OTU) were identified to species levels by mapping the generated V12 and V45 regions of 16 S rRNA gene to the SILVA database. These OTU tables were used to infer the microbial abundance in each sample type and at the individual animal level. The sequences for the corresponding OTUs were also used to generate phylogenetic trees and thus infer evolution for the OTUs of interest. Results: Here, we demonstrate that buffaloes harbor many bacteria in their blood. We also report a diversity of 16 S rRNA gene sequences for <i>Anaplasma</i> and <i>Mycoplasma</i> from individual animals. By sequencing both whole blood and serum in triplicate for each animal, we provide evidence of the differences in detecting bacteria in both sample types. Conclusions: Diverse bacteria, including some potential pathogens, can be found in the blood of clinically healthy wild African buffalo. Agnostic surveillance for such pathogens can be achieved through blood microbiome sequencing. However, considerations for the question being asked for the blood microbiome in wildlife will impact the choice for using whole blood or serum for sequencing. 2025-01-12 2025-01-16T06:16:22Z 2025-01-16T06:16:22Z Journal Article https://hdl.handle.net/10568/169188 en Open Access BioMed Central Nyamota, R., Middlebrook, E.A., Abkallo, H.M., Akoko, J., Gakuya, F., Wambua, L., Ronoh, B., Lekolool, I., Mwatondo, A., Muturi, M., Bett, B., Fair, J.M. and Bartlow, A.W. 2025. The bacterial and pathogenic landscape of African buffalo (<i>Syncerus caffer</i>) whole blood and serum from Kenya. Animal Microbiome 7: 6. |
| spellingShingle | african buffaloes diagnosis microbiomes Nyamota, Richard Middlebrook, E.A. Abkallo, Hussein M. Akoko, James M. Gakuya, F. Wambua, Lillian Ronoh, B. Lekolool, I. Mwatondo, Athman Muturi, Mathew Bett, Bernard K. Fair, J.M. Bartlow, A.W. The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title | The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title_full | The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title_fullStr | The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title_full_unstemmed | The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title_short | The bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya |
| title_sort | bacterial and pathogenic landscape of african buffalo syncerus caffer whole blood and serum from kenya |
| topic | african buffaloes diagnosis microbiomes |
| url | https://hdl.handle.net/10568/169188 |
| work_keys_str_mv | AT nyamotarichard thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT middlebrookea thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT abkallohusseinm thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT akokojamesm thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT gakuyaf thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT wambualillian thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT ronohb thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT lekolooli thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT mwatondoathman thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT muturimathew thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT bettbernardk thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT fairjm thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT bartlowaw thebacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT nyamotarichard bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT middlebrookea bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT abkallohusseinm bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT akokojamesm bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT gakuyaf bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT wambualillian bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT ronohb bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT lekolooli bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT mwatondoathman bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT muturimathew bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT bettbernardk bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT fairjm bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya AT bartlowaw bacterialandpathogeniclandscapeofafricanbuffalosynceruscafferwholebloodandserumfromkenya |