The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia
Contamination of milk is a serious public health risk, particularly in developing countries such as Ethiopia. Training is a tool for improving the quality and safety of milk. However, its effect on the microbial quality and safety of milk has not been well documented. This study assessed the impact...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/168542 |
| _version_ | 1855519400055013376 |
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| author | Beyene, A.M. Nigatu, S. Archila-Godinez, J.C. Amenu, Kebede Kowalcyk, B. Degefaw, D. Mogess, B. Gelaw, B. Gizachew, M. Mengistu, A. Abdelhamid, A.G. Barkley, J. Yousef, A. |
| author_browse | Abdelhamid, A.G. Amenu, Kebede Archila-Godinez, J.C. Barkley, J. Beyene, A.M. Degefaw, D. Gelaw, B. Gizachew, M. Kowalcyk, B. Mengistu, A. Mogess, B. Nigatu, S. Yousef, A. |
| author_facet | Beyene, A.M. Nigatu, S. Archila-Godinez, J.C. Amenu, Kebede Kowalcyk, B. Degefaw, D. Mogess, B. Gelaw, B. Gizachew, M. Mengistu, A. Abdelhamid, A.G. Barkley, J. Yousef, A. |
| author_sort | Beyene, A.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Contamination of milk is a serious public health risk, particularly in developing countries such as Ethiopia. Training is a tool for improving the quality and safety of milk. However, its effect on the microbial quality and safety of milk has not been well documented. This study assessed the impact of training interventions on the microbial quality and safety of milk, with a specific focus on smallholder dairy farms. The study was conducted from January to June 2022 in four locations in Central Ethiopia. Milk samples were collected from 120 dairy farmers one week before and four to six weeks after the training and analyzed for total coliforms, thermotolerant coliforms, <i>Escherichia coli</i>, Shiga toxin-producing <i>E. coli</i> (STEC), <i>Salmonella enterica</i> and <i>Campylobacter jejuni</i>. Total and thermotolerant coliforms were determined by the Most Probable Number (MPN) method; categorized as high (≥1001 MPN/ml), medium (101 to 103 MPN/ml), low (21 to 102 MPN/ml), and very low (≤20 MPN/ml); and analyzed using generalized linear mixed models. Prevalence of <i>E. coli</i>, STEC, <i>S. enterica</i>, and <i>C. jejuni</i> in raw milk samples were 67, 12, 3, and 4% pre-training and 45, 4, 3 and 2% post-training, respectively. The prevalence of <i>E. coli</i> (p = 0.0389) and STEC (p = 0.0005) was significantly lower post-training compared to pre-training. Most samples had total coliform counts exceeding 103 MPN/ml pre-training (71%); this proportion decreased to 62% post-training. The estimated cumulative probability of being in the high category was marginally significantly higher (p = 0.0581) pre-training (76%) compared to post-training (64%). In conclusion, reductions in the detection of some milk quality and safety parameters were observed. However, a considerable proportion of the milk samples were highly contaminated even after the training. Hence, comprehensive, and continued risk mitigation strategies are needed to ensure milk safety for consumers. |
| format | Journal Article |
| id | CGSpace168542 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1685422025-10-26T12:54:41Z The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia Beyene, A.M. Nigatu, S. Archila-Godinez, J.C. Amenu, Kebede Kowalcyk, B. Degefaw, D. Mogess, B. Gelaw, B. Gizachew, M. Mengistu, A. Abdelhamid, A.G. Barkley, J. Yousef, A. capacity building dairying food safety women Contamination of milk is a serious public health risk, particularly in developing countries such as Ethiopia. Training is a tool for improving the quality and safety of milk. However, its effect on the microbial quality and safety of milk has not been well documented. This study assessed the impact of training interventions on the microbial quality and safety of milk, with a specific focus on smallholder dairy farms. The study was conducted from January to June 2022 in four locations in Central Ethiopia. Milk samples were collected from 120 dairy farmers one week before and four to six weeks after the training and analyzed for total coliforms, thermotolerant coliforms, <i>Escherichia coli</i>, Shiga toxin-producing <i>E. coli</i> (STEC), <i>Salmonella enterica</i> and <i>Campylobacter jejuni</i>. Total and thermotolerant coliforms were determined by the Most Probable Number (MPN) method; categorized as high (≥1001 MPN/ml), medium (101 to 103 MPN/ml), low (21 to 102 MPN/ml), and very low (≤20 MPN/ml); and analyzed using generalized linear mixed models. Prevalence of <i>E. coli</i>, STEC, <i>S. enterica</i>, and <i>C. jejuni</i> in raw milk samples were 67, 12, 3, and 4% pre-training and 45, 4, 3 and 2% post-training, respectively. The prevalence of <i>E. coli</i> (p = 0.0389) and STEC (p = 0.0005) was significantly lower post-training compared to pre-training. Most samples had total coliform counts exceeding 103 MPN/ml pre-training (71%); this proportion decreased to 62% post-training. The estimated cumulative probability of being in the high category was marginally significantly higher (p = 0.0581) pre-training (76%) compared to post-training (64%). In conclusion, reductions in the detection of some milk quality and safety parameters were observed. However, a considerable proportion of the milk samples were highly contaminated even after the training. Hence, comprehensive, and continued risk mitigation strategies are needed to ensure milk safety for consumers. 2025-02 2025-01-06T09:43:44Z 2025-01-06T09:43:44Z Journal Article https://hdl.handle.net/10568/168542 en Open Access Elsevier Beyene, A.M., Nigatu, S., Archila-Godinez, J.C., Amenu, K., Kowalcyk, B., Degefaw, D., Mogess, B., Gelaw, B., Gizachew, M., Mengistu, A., Abdelhamid, A.G., Barkley, J. and Yousef, A. 2025. The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia. Journal of Food Protection 88(2): 100446. |
| spellingShingle | capacity building dairying food safety women Beyene, A.M. Nigatu, S. Archila-Godinez, J.C. Amenu, Kebede Kowalcyk, B. Degefaw, D. Mogess, B. Gelaw, B. Gizachew, M. Mengistu, A. Abdelhamid, A.G. Barkley, J. Yousef, A. The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title | The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title_full | The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title_fullStr | The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title_full_unstemmed | The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title_short | The impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in Central Ethiopia |
| title_sort | impact of training intervention on levels of indicator bacteria and prevalence of selected pathogens in raw milk from smallholder women dairy farmers in central ethiopia |
| topic | capacity building dairying food safety women |
| url | https://hdl.handle.net/10568/168542 |
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