Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India
Fusarium wilt of banana is a major production constraint in India, prompting banana growers to replace bananas with less remunerative crops. Effective disease management practices thus need to be developed and implemented to prevent further spread and damage caused by Fusarium oxysporum f. sp. cuben...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2024
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/168259 |
| _version_ | 1855525769118220288 |
|---|---|
| author | Thangavelu, Raman Amaresh, Hadimani Gopi, Muthukathan Loganathan, Murugan Nithya, Boopathy Devi, Perumal Ganga Anuradha, Chelliah Thirugnanavel, Anbazhagan Patil, Kalyansing Baburao Blomme, Guy Selvarajan, Ramasamy |
| author_browse | Amaresh, Hadimani Anuradha, Chelliah Blomme, Guy Devi, Perumal Ganga Gopi, Muthukathan Loganathan, Murugan Nithya, Boopathy Patil, Kalyansing Baburao Selvarajan, Ramasamy Thangavelu, Raman Thirugnanavel, Anbazhagan |
| author_facet | Thangavelu, Raman Amaresh, Hadimani Gopi, Muthukathan Loganathan, Murugan Nithya, Boopathy Devi, Perumal Ganga Anuradha, Chelliah Thirugnanavel, Anbazhagan Patil, Kalyansing Baburao Blomme, Guy Selvarajan, Ramasamy |
| author_sort | Thangavelu, Raman |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fusarium wilt of banana is a major production constraint in India, prompting banana growers to replace bananas with less remunerative crops. Effective disease management practices thus need to be developed and implemented to prevent further spread and damage caused by Fusarium oxysporum f. sp. cubense (Foc), the cause of Fusarium wilt. Currently, knowledge of disease incidence, affected varieties, and the geographical spread of Foc races in India are only scantily available. An extensive field survey was conducted in 53 districts of 16 major banana-growing states of and one union territory of India that covered both tropical and subtropical regions. Disease incidence ranged from 0 to 95% on farms, with Cavendish bananas (AAA) most affected. No Fusarium wilt symptoms due to Foc R1 were observed in Nendran (AAB) or Red Banana (AAA) in South India. During the survey, 293 Foc isolates were collected from Cavendish, Pisang Awak (ABB), Silk (AAB), Monthan (ABB), Ney poovan (AB), and Mysore (AAB) bananas. Isolate diversity was assessed through Vegetative Compatibility Group (VCG) analyses, sequencing of EF1α gene sequences, phylogenetic analyses, and characterisation by SIX gene composition. Thirteen VCGs were identified, of which VCGs 0124, 0125, 01220, and 01213/16 were dominant and infected Cavendish bananas. Phylogenetic analysis divided the Indian Foc isolates into race 1 (R1), subtropical race 4 (STR4), and tropical race 4 (TR4). Secreted in Xylem (SIX) gene analyses indicated that the effector genes SIX4 and SIX6 were present in the VCGs 0124, 0124/5, 0125, and 01220 of race 1, SIX7 was present only in Foc STR4, and SIX8 was found only in Foc R4 (TR4 and STR4) isolates. Insights into the geographical distribution of Foc races, and their interactions with banana varieties, can guide integrated disease management intervention strategies across India. |
| format | Journal Article |
| id | CGSpace168259 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1682592025-12-08T10:29:22Z Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India Thangavelu, Raman Amaresh, Hadimani Gopi, Muthukathan Loganathan, Murugan Nithya, Boopathy Devi, Perumal Ganga Anuradha, Chelliah Thirugnanavel, Anbazhagan Patil, Kalyansing Baburao Blomme, Guy Selvarajan, Ramasamy genetic diversity (as resource) bananas geographical distribution fusarium host range Fusarium wilt of banana is a major production constraint in India, prompting banana growers to replace bananas with less remunerative crops. Effective disease management practices thus need to be developed and implemented to prevent further spread and damage caused by Fusarium oxysporum f. sp. cubense (Foc), the cause of Fusarium wilt. Currently, knowledge of disease incidence, affected varieties, and the geographical spread of Foc races in India are only scantily available. An extensive field survey was conducted in 53 districts of 16 major banana-growing states of and one union territory of India that covered both tropical and subtropical regions. Disease incidence ranged from 0 to 95% on farms, with Cavendish bananas (AAA) most affected. No Fusarium wilt symptoms due to Foc R1 were observed in Nendran (AAB) or Red Banana (AAA) in South India. During the survey, 293 Foc isolates were collected from Cavendish, Pisang Awak (ABB), Silk (AAB), Monthan (ABB), Ney poovan (AB), and Mysore (AAB) bananas. Isolate diversity was assessed through Vegetative Compatibility Group (VCG) analyses, sequencing of EF1α gene sequences, phylogenetic analyses, and characterisation by SIX gene composition. Thirteen VCGs were identified, of which VCGs 0124, 0125, 01220, and 01213/16 were dominant and infected Cavendish bananas. Phylogenetic analysis divided the Indian Foc isolates into race 1 (R1), subtropical race 4 (STR4), and tropical race 4 (TR4). Secreted in Xylem (SIX) gene analyses indicated that the effector genes SIX4 and SIX6 were present in the VCGs 0124, 0124/5, 0125, and 01220 of race 1, SIX7 was present only in Foc STR4, and SIX8 was found only in Foc R4 (TR4 and STR4) isolates. Insights into the geographical distribution of Foc races, and their interactions with banana varieties, can guide integrated disease management intervention strategies across India. 2024-12 2024-12-21T15:37:45Z 2024-12-21T15:37:45Z Journal Article https://hdl.handle.net/10568/168259 en Open Access application/pdf MDPI Thangavelu, R.; Amaresh, H.; Gopi, M.; Loganathan, M.; Nithya, B.; Devi, P.G.; Anuradha, C.; Thirugnanavel, A.; Patil, K.B.; Blomme, G.; Selvarajan, R. (2024) Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India. Journal of Fungi 10(12): 887. ISSN: 2309-608X |
| spellingShingle | genetic diversity (as resource) bananas geographical distribution fusarium host range Thangavelu, Raman Amaresh, Hadimani Gopi, Muthukathan Loganathan, Murugan Nithya, Boopathy Devi, Perumal Ganga Anuradha, Chelliah Thirugnanavel, Anbazhagan Patil, Kalyansing Baburao Blomme, Guy Selvarajan, Ramasamy Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title | Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title_full | Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title_fullStr | Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title_full_unstemmed | Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title_short | Geographical distribution, host range and genetic diversity of Fusarium oxysporum f. sp. cubense causing Fusarium wilt of banana in India |
| title_sort | geographical distribution host range and genetic diversity of fusarium oxysporum f sp cubense causing fusarium wilt of banana in india |
| topic | genetic diversity (as resource) bananas geographical distribution fusarium host range |
| url | https://hdl.handle.net/10568/168259 |
| work_keys_str_mv | AT thangaveluraman geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT amareshhadimani geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT gopimuthukathan geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT loganathanmurugan geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT nithyaboopathy geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT deviperumalganga geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT anuradhachelliah geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT thirugnanavelanbazhagan geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT patilkalyansingbaburao geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT blommeguy geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia AT selvarajanramasamy geographicaldistributionhostrangeandgeneticdiversityoffusariumoxysporumfspcubensecausingfusariumwiltofbananainindia |