Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA)
The serological relationship of several, mainly Gram-negative, bacteria including type cultures and previously isolated rhizosphere bacteria, to Pseudomonas sp. strain H8, a N2-fixing rhizosphere isolate from Maahas soil, was examined by indirect enzyme-linked immunosorbent assay. None cross-reacted...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Canadian Science Publishing
1986
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/167746 |
| _version_ | 1855542373277237248 |
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| author | Barraquio, W.L. Ladha, J.K. Yao, H.Q. Watanabe, I. |
| author_browse | Barraquio, W.L. Ladha, J.K. Watanabe, I. Yao, H.Q. |
| author_facet | Barraquio, W.L. Ladha, J.K. Yao, H.Q. Watanabe, I. |
| author_sort | Barraquio, W.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The serological relationship of several, mainly Gram-negative, bacteria including type cultures and previously isolated rhizosphere bacteria, to Pseudomonas sp. strain H8, a N2-fixing rhizosphere isolate from Maahas soil, was examined by indirect enzyme-linked immunosorbent assay. None cross-reacted significantly with H8 antibody except some of those rhizosphere bacteria previously found cross-reactive by fluorescent antibody test. Several new rhizosphere isolates from wetland rice grown in various fields showed significant immunoreactivity, and most of the reactive bacteria reduced acetylene. However, the cultural characteristics and acetylene reduction activity of most of these isolates were different from those of H8. None of the rhizosphere isolates from dryland rice cross-reacted. In a pot experiment, bacterial cultures similar to H8 were found only in Maahas soil, but none reacted with antibodies against strains H8 and KLH76 (another N2-fixing pseudomonad from rice root). Other isolates showing reaction with these antibodies were, however, neither culturally similar to these strains nor N2 fixing. The data suggest that Pseudomonas sp. H8 is not distributed widely in soils other than that from which it was originally isolated. |
| format | Journal Article |
| id | CGSpace167746 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1986 |
| publishDateRange | 1986 |
| publishDateSort | 1986 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace1677462025-05-14T10:40:02Z Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Barraquio, W.L. Ladha, J.K. Yao, H.Q. Watanabe, I. n2 fixing pseudomonas strain h8 rhizosphere enzyme linked immunosorbent assay The serological relationship of several, mainly Gram-negative, bacteria including type cultures and previously isolated rhizosphere bacteria, to Pseudomonas sp. strain H8, a N2-fixing rhizosphere isolate from Maahas soil, was examined by indirect enzyme-linked immunosorbent assay. None cross-reacted significantly with H8 antibody except some of those rhizosphere bacteria previously found cross-reactive by fluorescent antibody test. Several new rhizosphere isolates from wetland rice grown in various fields showed significant immunoreactivity, and most of the reactive bacteria reduced acetylene. However, the cultural characteristics and acetylene reduction activity of most of these isolates were different from those of H8. None of the rhizosphere isolates from dryland rice cross-reacted. In a pot experiment, bacterial cultures similar to H8 were found only in Maahas soil, but none reacted with antibodies against strains H8 and KLH76 (another N2-fixing pseudomonad from rice root). Other isolates showing reaction with these antibodies were, however, neither culturally similar to these strains nor N2 fixing. The data suggest that Pseudomonas sp. H8 is not distributed widely in soils other than that from which it was originally isolated. 1986-05-01 2024-12-19T12:57:40Z 2024-12-19T12:57:40Z Journal Article https://hdl.handle.net/10568/167746 en Canadian Science Publishing Barraquio, W. L.; Ladha, J. K.; Yao, H. Q. and Watanabe, I. 1986. Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA). Can. J. Microbiol., Volume 32 no. 5 p. 402-408 |
| spellingShingle | n2 fixing pseudomonas strain h8 rhizosphere enzyme linked immunosorbent assay Barraquio, W.L. Ladha, J.K. Yao, H.Q. Watanabe, I. Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title | Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title_full | Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title_fullStr | Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title_full_unstemmed | Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title_short | Antigenic Relationship of N2-Fixing Pseudomonas Strain H8 to Various Known Cultures and Rice Rhizosphere Isolates Studied By Indirect Enzyme-Linked Immunosorbent Assay (ELISA) |
| title_sort | antigenic relationship of n2 fixing pseudomonas strain h8 to various known cultures and rice rhizosphere isolates studied by indirect enzyme linked immunosorbent assay elisa |
| topic | n2 fixing pseudomonas strain h8 rhizosphere enzyme linked immunosorbent assay |
| url | https://hdl.handle.net/10568/167746 |
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