Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior

Interspecific hybrids between three breeding lines of Oryza sativa (2n = 24 = AA) and 18 accessions of O. officinalis (2n = 24 = CC) were obtained through an embryo-rescue technique. The crossability ranged from 1.0 to 2.3%. The AC hybrids showed a low amount of chromosome pairing (0–4 bivalents) an...

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Main Authors: Jena, K.K., Khush, Gurdev S.
Format: Journal Article
Language:Inglés
Published: Canadian Science Publishing 1989
Subjects:
Online Access:https://hdl.handle.net/10568/167618
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author Jena, K.K.
Khush, Gurdev S.
author_browse Jena, K.K.
Khush, Gurdev S.
author_facet Jena, K.K.
Khush, Gurdev S.
author_sort Jena, K.K.
collection Repository of Agricultural Research Outputs (CGSpace)
description Interspecific hybrids between three breeding lines of Oryza sativa (2n = 24 = AA) and 18 accessions of O. officinalis (2n = 24 = CC) were obtained through an embryo-rescue technique. The crossability ranged from 1.0 to 2.3%. The AC hybrids showed a low amount of chromosome pairing (0–4 bivalents) and were completely male sterile. The BC1 progeny, obtained upon backcrossing to O. sativa were allotriploid (AAC) and were also male sterile. Further backcrosses to O. sativa produced 94 BC2 plants with chromosome numbers of 2n to 2n + 6. Monosomic alien addition lines (AA + 1C) corresponding to the 12 chromosomes of the haploid complement of O. officinalis were isolated from among forty 2n + 1 BC2 plants. These addition lines differ from their diploid sibs by an array of morphological features and resemble the 12 primary trisomics of O. sativa. The female transmission rates of alien chromosomes varied from 6.6 to 26.8%. Four of the 12 monosomic alien addition lines transmitted the alien chromosome through the male gametes.Key words: chromosome pairing, introgression, embryo rescue, allotriploid, genes for insect resistance, interspecific gene transfer.
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spelling CGSpace1676182025-05-14T10:39:33Z Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior Jena, K.K. Khush, Gurdev S. monosomic alien addition lines chromosome pairing introgression embryo rescue allotriploid Interspecific hybrids between three breeding lines of Oryza sativa (2n = 24 = AA) and 18 accessions of O. officinalis (2n = 24 = CC) were obtained through an embryo-rescue technique. The crossability ranged from 1.0 to 2.3%. The AC hybrids showed a low amount of chromosome pairing (0–4 bivalents) and were completely male sterile. The BC1 progeny, obtained upon backcrossing to O. sativa were allotriploid (AAC) and were also male sterile. Further backcrosses to O. sativa produced 94 BC2 plants with chromosome numbers of 2n to 2n + 6. Monosomic alien addition lines (AA + 1C) corresponding to the 12 chromosomes of the haploid complement of O. officinalis were isolated from among forty 2n + 1 BC2 plants. These addition lines differ from their diploid sibs by an array of morphological features and resemble the 12 primary trisomics of O. sativa. The female transmission rates of alien chromosomes varied from 6.6 to 26.8%. Four of the 12 monosomic alien addition lines transmitted the alien chromosome through the male gametes.Key words: chromosome pairing, introgression, embryo rescue, allotriploid, genes for insect resistance, interspecific gene transfer. 1989-06-01 2024-12-19T12:57:33Z 2024-12-19T12:57:33Z Journal Article https://hdl.handle.net/10568/167618 en Canadian Science Publishing Jena, K. K.; Khush, Gurdev S. 1989. Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior. Genome, Volume 32 no. 3 p. 449-455
spellingShingle monosomic alien addition lines
chromosome pairing
introgression
embryo rescue
allotriploid
Jena, K.K.
Khush, Gurdev S.
Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title_full Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title_fullStr Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title_full_unstemmed Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title_short Monosomic Alien Addition Lines of Rice: Production, Morphology, Cytology, and Breeding Behavior
title_sort monosomic alien addition lines of rice production morphology cytology and breeding behavior
topic monosomic alien addition lines
chromosome pairing
introgression
embryo rescue
allotriploid
url https://hdl.handle.net/10568/167618
work_keys_str_mv AT jenakk monosomicalienadditionlinesofriceproductionmorphologycytologyandbreedingbehavior
AT khushgurdevs monosomicalienadditionlinesofriceproductionmorphologycytologyandbreedingbehavior