Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture

Low efficiencies of callus induction and green‐plant regeneration have limited the application of anther culture in breeding programs. This study was conducted to determine the inheritance of callus induction and plant regeneration in rice (Oryza sativa L.) anther culture. Sixteen genotypes derived...

Descripción completa

Detalles Bibliográficos
Autores principales: Quimio, C.A., Zapata, F.J.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 1990
Materias:
Acceso en línea:https://hdl.handle.net/10568/167594
_version_ 1855518480159211520
author Quimio, C.A.
Zapata, F.J.
author_browse Quimio, C.A.
Zapata, F.J.
author_facet Quimio, C.A.
Zapata, F.J.
author_sort Quimio, C.A.
collection Repository of Agricultural Research Outputs (CGSpace)
description Low efficiencies of callus induction and green‐plant regeneration have limited the application of anther culture in breeding programs. This study was conducted to determine the inheritance of callus induction and plant regeneration in rice (Oryza sativa L.) anther culture. Sixteen genotypes derived from a four‐parent diallel mating set of two Japonica cultivars (Taipei 309 and Taipei 177) and two indica cultivars (IR36 and Basmati 370) were evaluated for their callus induction and plant regeneration abilities. For callus induction, the effects of genotype and genotype ✕ medium interaction were significant (P < 0.05). Similarly, genotype and abscisic acid (ABA) level were found to be significant (P < 0.05) for green‐plant production. The japonica cultivars generally had a higher percent callus induction and plant regeneration, and number of calli producing green plants than did the indica cultivars. Similar modes of inheritance were observed for both characters. Combining ability analysis demonstrated the predominance of additive gene effects in the control of both characters with the japonica cultivars having higher combining ability for green‐plant regeneration. Reciprocal effects were not significant (P > 0.05), suggesting the absence of maternal control on the characters. Further analysis showed gene action to be partially dominant with high response being highly recessive and controlled by a few genes. There were no indications of inter‐allelic interaction.
format Journal Article
id CGSpace167594
institution CGIAR Consortium
language Inglés
publishDate 1990
publishDateRange 1990
publishDateSort 1990
publisher Wiley
publisherStr Wiley
record_format dspace
spelling CGSpace1675942025-05-14T10:39:33Z Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture Quimio, C.A. Zapata, F.J. diallel analysis callus induction plant regeneration anther culture Low efficiencies of callus induction and green‐plant regeneration have limited the application of anther culture in breeding programs. This study was conducted to determine the inheritance of callus induction and plant regeneration in rice (Oryza sativa L.) anther culture. Sixteen genotypes derived from a four‐parent diallel mating set of two Japonica cultivars (Taipei 309 and Taipei 177) and two indica cultivars (IR36 and Basmati 370) were evaluated for their callus induction and plant regeneration abilities. For callus induction, the effects of genotype and genotype ✕ medium interaction were significant (P < 0.05). Similarly, genotype and abscisic acid (ABA) level were found to be significant (P < 0.05) for green‐plant production. The japonica cultivars generally had a higher percent callus induction and plant regeneration, and number of calli producing green plants than did the indica cultivars. Similar modes of inheritance were observed for both characters. Combining ability analysis demonstrated the predominance of additive gene effects in the control of both characters with the japonica cultivars having higher combining ability for green‐plant regeneration. Reciprocal effects were not significant (P > 0.05), suggesting the absence of maternal control on the characters. Further analysis showed gene action to be partially dominant with high response being highly recessive and controlled by a few genes. There were no indications of inter‐allelic interaction. 1990-01 2024-12-19T12:57:32Z 2024-12-19T12:57:32Z Journal Article https://hdl.handle.net/10568/167594 en Wiley Quimio, C. A.; Zapata, F. J. 1990. Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture. Crop Science, Volume 30 no. 1 p. 188-192
spellingShingle diallel analysis
callus induction
plant regeneration
anther culture
Quimio, C.A.
Zapata, F.J.
Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title_full Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title_fullStr Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title_full_unstemmed Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title_short Diallel Analysis of Callus Induction and Green-Plant Regeneration in Rice Anther Culture
title_sort diallel analysis of callus induction and green plant regeneration in rice anther culture
topic diallel analysis
callus induction
plant regeneration
anther culture
url https://hdl.handle.net/10568/167594
work_keys_str_mv AT quimioca diallelanalysisofcallusinductionandgreenplantregenerationinriceantherculture
AT zapatafj diallelanalysisofcallusinductionandgreenplantregenerationinriceantherculture