Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers
The polymerase chain reaction was used to amplify random sequences of DNA from 25 accessions of Azolla to evaluate the usefulness of this technique for identification and phylogenetic analysis of this aquatic fern. Accessions were selected to represent all known species within the genus Azolla and t...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Canadian Science Publishing
1993
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| Online Access: | https://hdl.handle.net/10568/167451 |
| _version_ | 1855543248808837120 |
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| author | Coppenolle, Benoit Van Watanabe, Iwao Hove, Charles Van Second, Gerard Huang, Ning McCouch, Susan R. |
| author_browse | Coppenolle, Benoit Van Hove, Charles Van Huang, Ning McCouch, Susan R. Second, Gerard Watanabe, Iwao |
| author_facet | Coppenolle, Benoit Van Watanabe, Iwao Hove, Charles Van Second, Gerard Huang, Ning McCouch, Susan R. |
| author_sort | Coppenolle, Benoit Van |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The polymerase chain reaction was used to amplify random sequences of DNA from 25 accessions of Azolla to evaluate the usefulness of this technique for identification and phylogenetic analysis of this aquatic fern. Accessions were selected to represent all known species within the genus Azolla and to encompass the worldwide distribution of the fern. Primers of 10 nucleotides with 70% G + C content were used to generate randomly amplified polymorphic DNA from the symbiotic Azolla–Anabaena complex. Twenty-two primers were used and each primer gave 4–10 bands of different molecular weights for each accession. Bands were scored as present or absent for each accession and variation among accessions was quantified using Nei's genetic distances. A dendrogram summarizing phenetic relationships among the 25 accessions was generated using the unweighted pair-group method with arithmetic mean. Principal component analysis was also used to evaluate genetic similarities. Three distinct groups were identified: group 1 contains five species, group 2 contains the pinnata species, and group 3 contains the nilotica species. The analysis demonstrates that the major groups of Azolla species can be easily distinguished from one an other and, in addition, that closely related accessions within species can be identified. We further found that using 10 primers, a phylogeny that is essentially the same as that derived from 22 primers can be constructed. Our results suggest that total DNA extracted from the Azolla–Anabaena symbionts is useful for classification and phylogenetic studies of Azolla.Key words: Azolla–Anabaena symbiosis, genetic distances, polymerase chain reaction, principal component analysis. |
| format | Journal Article |
| id | CGSpace167451 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace1674512024-12-19T14:13:49Z Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers Coppenolle, Benoit Van Watanabe, Iwao Hove, Charles Van Second, Gerard Huang, Ning McCouch, Susan R. The polymerase chain reaction was used to amplify random sequences of DNA from 25 accessions of Azolla to evaluate the usefulness of this technique for identification and phylogenetic analysis of this aquatic fern. Accessions were selected to represent all known species within the genus Azolla and to encompass the worldwide distribution of the fern. Primers of 10 nucleotides with 70% G + C content were used to generate randomly amplified polymorphic DNA from the symbiotic Azolla–Anabaena complex. Twenty-two primers were used and each primer gave 4–10 bands of different molecular weights for each accession. Bands were scored as present or absent for each accession and variation among accessions was quantified using Nei's genetic distances. A dendrogram summarizing phenetic relationships among the 25 accessions was generated using the unweighted pair-group method with arithmetic mean. Principal component analysis was also used to evaluate genetic similarities. Three distinct groups were identified: group 1 contains five species, group 2 contains the pinnata species, and group 3 contains the nilotica species. The analysis demonstrates that the major groups of Azolla species can be easily distinguished from one an other and, in addition, that closely related accessions within species can be identified. We further found that using 10 primers, a phylogeny that is essentially the same as that derived from 22 primers can be constructed. Our results suggest that total DNA extracted from the Azolla–Anabaena symbionts is useful for classification and phylogenetic studies of Azolla.Key words: Azolla–Anabaena symbiosis, genetic distances, polymerase chain reaction, principal component analysis. 1993-08-01 2024-12-19T12:57:23Z 2024-12-19T12:57:23Z Journal Article https://hdl.handle.net/10568/167451 en Canadian Science Publishing Coppenolle, Benoit Van; Watanabe, Iwao; Hove, Charles Van; Second, Gerard; Huang, Ning and McCouch, Susan R. 1993. Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers. Genome, Volume 36 no. 4 p. 686-693 |
| spellingShingle | Coppenolle, Benoit Van Watanabe, Iwao Hove, Charles Van Second, Gerard Huang, Ning McCouch, Susan R. Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title | Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title_full | Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title_fullStr | Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title_full_unstemmed | Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title_short | Genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| title_sort | genetic diversity and phylogeny analysis of azolla based on dna amplification by arbitrary primers |
| url | https://hdl.handle.net/10568/167451 |
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