Nondestructive estimation of shoot nitrogen in different rice genotypes

Plant N uptake and grain yield are important components of N use efficiency. Grain yield is easily measured, but plant N analysis is time consuming and requires hazardous chemicals or expensive equipment. A nondestructive method involving the least equipment and skill, to determine N uptake, is need...

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Main Authors: Ladha, Jagdish K., Tirol-Padre, Agnes, Punzalan, Gloria C., Castillo, E., Singh, Upendra, Reddy, C. Kesava
Format: Journal Article
Language:Inglés
Published: Wiley 1998
Subjects:
Online Access:https://hdl.handle.net/10568/167225
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author Ladha, Jagdish K.
Tirol-Padre, Agnes
Punzalan, Gloria C.
Castillo, E.
Singh, Upendra
Reddy, C. Kesava
author_browse Castillo, E.
Ladha, Jagdish K.
Punzalan, Gloria C.
Reddy, C. Kesava
Singh, Upendra
Tirol-Padre, Agnes
author_facet Ladha, Jagdish K.
Tirol-Padre, Agnes
Punzalan, Gloria C.
Castillo, E.
Singh, Upendra
Reddy, C. Kesava
author_sort Ladha, Jagdish K.
collection Repository of Agricultural Research Outputs (CGSpace)
description Plant N uptake and grain yield are important components of N use efficiency. Grain yield is easily measured, but plant N analysis is time consuming and requires hazardous chemicals or expensive equipment. A nondestructive method involving the least equipment and skill, to determine N uptake, is needed in agronomic and plant breeding experiments. A nondestructive method to determine shoot (aboveground biomass) N of transplanted rice (Oryza sativa L.) was developed based on SPAD‐502 chlorophyll meter readings, leaf area, and tiller number. In two dry‐season and one wet‐season field experiment, shoot N of various genotypes at flowering were highly correlated (P < 0.05) with CLAT, the product of SPAD reading from a selected leaf (C), area of that leaf (LA), and number of tillers (T) (r2 = 0.46, 0.90, and 0.85 in Exp. 1, 2, and 3, respectively); and with LAT, the product of LA and T (r2 = 0.56, 0.88, and 0.76). Shoot N may be estimated using LAT for larger differences in leaf area compared with SPAD readings. Lower correlations in Exp. 1 were due to the lower range in shoot N contents. The highest correlation between shoot N and CLAT was observed in the third uppermost leaf. Regressions of shoot N on LAT and CLAT varied across growth stages and seasons. Thus, LAT or CLAT can be used to evaluate N uptake among N fertilizer treatments and different rice genotypes at a given stage within a season. Further work is needed to assess the reliability of this method under different seasons and cultural practices.
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spelling CGSpace1672252025-05-14T10:24:30Z Nondestructive estimation of shoot nitrogen in different rice genotypes Ladha, Jagdish K. Tirol-Padre, Agnes Punzalan, Gloria C. Castillo, E. Singh, Upendra Reddy, C. Kesava nitrogen uptake shoots genotypes nondestructive testing regression analysis Plant N uptake and grain yield are important components of N use efficiency. Grain yield is easily measured, but plant N analysis is time consuming and requires hazardous chemicals or expensive equipment. A nondestructive method involving the least equipment and skill, to determine N uptake, is needed in agronomic and plant breeding experiments. A nondestructive method to determine shoot (aboveground biomass) N of transplanted rice (Oryza sativa L.) was developed based on SPAD‐502 chlorophyll meter readings, leaf area, and tiller number. In two dry‐season and one wet‐season field experiment, shoot N of various genotypes at flowering were highly correlated (P < 0.05) with CLAT, the product of SPAD reading from a selected leaf (C), area of that leaf (LA), and number of tillers (T) (r2 = 0.46, 0.90, and 0.85 in Exp. 1, 2, and 3, respectively); and with LAT, the product of LA and T (r2 = 0.56, 0.88, and 0.76). Shoot N may be estimated using LAT for larger differences in leaf area compared with SPAD readings. Lower correlations in Exp. 1 were due to the lower range in shoot N contents. The highest correlation between shoot N and CLAT was observed in the third uppermost leaf. Regressions of shoot N on LAT and CLAT varied across growth stages and seasons. Thus, LAT or CLAT can be used to evaluate N uptake among N fertilizer treatments and different rice genotypes at a given stage within a season. Further work is needed to assess the reliability of this method under different seasons and cultural practices. 1998-01 2024-12-19T12:57:09Z 2024-12-19T12:57:09Z Journal Article https://hdl.handle.net/10568/167225 en Wiley Ladha, Jagdish K.; Tirol‐Padre, Agnes; Punzalan, Gloria C.; Castillo, E.; Singh, Upendra and Reddy, C. Kesava. 1998. Nondestructive estimation of shoot nitrogen in different rice genotypes. Agronomy Journal, Volume 90 no. 1 p. 33-40
spellingShingle nitrogen uptake
shoots
genotypes
nondestructive testing
regression analysis
Ladha, Jagdish K.
Tirol-Padre, Agnes
Punzalan, Gloria C.
Castillo, E.
Singh, Upendra
Reddy, C. Kesava
Nondestructive estimation of shoot nitrogen in different rice genotypes
title Nondestructive estimation of shoot nitrogen in different rice genotypes
title_full Nondestructive estimation of shoot nitrogen in different rice genotypes
title_fullStr Nondestructive estimation of shoot nitrogen in different rice genotypes
title_full_unstemmed Nondestructive estimation of shoot nitrogen in different rice genotypes
title_short Nondestructive estimation of shoot nitrogen in different rice genotypes
title_sort nondestructive estimation of shoot nitrogen in different rice genotypes
topic nitrogen uptake
shoots
genotypes
nondestructive testing
regression analysis
url https://hdl.handle.net/10568/167225
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