Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola
Molecular tools were used to analyze the genetic diversity and population structure of Xanthomonas oryzae pv. oryzicola, the bacterial leaf streak pathogen of rice in the Philippines. Representative pathogen strains were selected and used to assess resistance in rice germplasm. A partial genomic lib...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Societies
1999
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/167150 |
| _version_ | 1855527128387289088 |
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| author | Raymundo, A.K. Briones, A.M. Ardales, E.Y. Perez, M.T. Fernandez, L.C. Leach, J.E. Mew, T.W. Ynalvez, M.A. McLaren, C.G. Nelson, R.J. |
| author_browse | Ardales, E.Y. Briones, A.M. Fernandez, L.C. Leach, J.E. McLaren, C.G. Mew, T.W. Nelson, R.J. Perez, M.T. Raymundo, A.K. Ynalvez, M.A. |
| author_facet | Raymundo, A.K. Briones, A.M. Ardales, E.Y. Perez, M.T. Fernandez, L.C. Leach, J.E. Mew, T.W. Ynalvez, M.A. McLaren, C.G. Nelson, R.J. |
| author_sort | Raymundo, A.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Molecular tools were used to analyze the genetic diversity and population structure of Xanthomonas oryzae pv. oryzicola, the bacterial leaf streak pathogen of rice in the Philippines. Representative pathogen strains were selected and used to assess resistance in rice germplasm. A partial genomic library of X. oryzae pv. oryzicola was constructed, and a 459-bp clone containing the repetitive DNA element R41 was selected as a probe for restriction fragment length polymorphism (RFLP) analysis and sequenced. R41 shared 44% sequence homology with the putative transposase gene of IS1112, an insertion element cloned from X. oryzae pv. oryzae. Using R41 as a probe for RFLP analysis, 26 band profiles were discerned in a collection of 123 strains of X. oryzae pv. oryzicola. Analysis of PstI digestion patterns of DNA from the same collection resolved 36 haplotypes. Several clusters of strains were detected after grouping of data based on either pR41 as a probe or Pst1 digestion patterns. However, based on bootstrap analysis, the clusters were not robust. Genetic diversity was high for the entire collection as well as within spatially and temporally defined subsets of strains. Even a set of strains collected from a single site at a single time was highly diverse. Strains representing the different DNA types were inoculated to a set of diverserice cultivars. Consistent rice varietal groupings were obtained from disease reaction data, but there was no correlation between pathogen isolate cluster and host reaction across inoculation trials. Isozyme group I of rice, representing tropical japonica and javanica germplasm, is a promising source of resistance to bacterial leaf streak. |
| format | Journal Article |
| id | CGSpace167150 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1671502025-05-14T10:39:44Z Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola Raymundo, A.K. Briones, A.M. Ardales, E.Y. Perez, M.T. Fernandez, L.C. Leach, J.E. Mew, T.W. Ynalvez, M.A. McLaren, C.G. Nelson, R.J. xanthomonas oryzae pv oryzicola bacterial leaf streak genetic diversity population structure virulence dna fingerprinting restriction fragment length polymorphism philippines Molecular tools were used to analyze the genetic diversity and population structure of Xanthomonas oryzae pv. oryzicola, the bacterial leaf streak pathogen of rice in the Philippines. Representative pathogen strains were selected and used to assess resistance in rice germplasm. A partial genomic library of X. oryzae pv. oryzicola was constructed, and a 459-bp clone containing the repetitive DNA element R41 was selected as a probe for restriction fragment length polymorphism (RFLP) analysis and sequenced. R41 shared 44% sequence homology with the putative transposase gene of IS1112, an insertion element cloned from X. oryzae pv. oryzae. Using R41 as a probe for RFLP analysis, 26 band profiles were discerned in a collection of 123 strains of X. oryzae pv. oryzicola. Analysis of PstI digestion patterns of DNA from the same collection resolved 36 haplotypes. Several clusters of strains were detected after grouping of data based on either pR41 as a probe or Pst1 digestion patterns. However, based on bootstrap analysis, the clusters were not robust. Genetic diversity was high for the entire collection as well as within spatially and temporally defined subsets of strains. Even a set of strains collected from a single site at a single time was highly diverse. Strains representing the different DNA types were inoculated to a set of diverserice cultivars. Consistent rice varietal groupings were obtained from disease reaction data, but there was no correlation between pathogen isolate cluster and host reaction across inoculation trials. Isozyme group I of rice, representing tropical japonica and javanica germplasm, is a promising source of resistance to bacterial leaf streak. 1999-05 2024-12-19T12:57:03Z 2024-12-19T12:57:03Z Journal Article https://hdl.handle.net/10568/167150 en Scientific Societies Raymundo, A. K.; Briones, A. M.; Ardales, E. Y.; Perez, M. T.; Fernandez, L. C.; Leach, J. E.; Mew, T. W.; Ynalvez, M. A.; McLaren, C. G. and Nelson, R. J. 1999. Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola. Plant Disease, Volume 83 no. 5 p. 434-440 |
| spellingShingle | xanthomonas oryzae pv oryzicola bacterial leaf streak genetic diversity population structure virulence dna fingerprinting restriction fragment length polymorphism philippines Raymundo, A.K. Briones, A.M. Ardales, E.Y. Perez, M.T. Fernandez, L.C. Leach, J.E. Mew, T.W. Ynalvez, M.A. McLaren, C.G. Nelson, R.J. Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title | Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title_full | Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title_fullStr | Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title_full_unstemmed | Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title_short | Analysis of DNA polymorphism and virulence in Philippine strains of Xanthomonas oryzae pv. oryzicola |
| title_sort | analysis of dna polymorphism and virulence in philippine strains of xanthomonas oryzae pv oryzicola |
| topic | xanthomonas oryzae pv oryzicola bacterial leaf streak genetic diversity population structure virulence dna fingerprinting restriction fragment length polymorphism philippines |
| url | https://hdl.handle.net/10568/167150 |
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