An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits
An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 2...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Canadian Science Publishing
2002
|
| Acceso en línea: | https://hdl.handle.net/10568/166984 |
| _version_ | 1855521257195307008 |
|---|---|
| author | Ouédraogo, J.T. Gowda, B.S. Jean, M. Close, T.J. Ehlers, J.D. Hall, A.E. Gillaspie, A.G. Roberts, P.A. Ismail, Abdelbagi M. Bruening, G. Gepts, P. Timko, M.P. Belzile, F.J. |
| author_browse | Belzile, F.J. Bruening, G. Close, T.J. Ehlers, J.D. Gepts, P. Gillaspie, A.G. Gowda, B.S. Hall, A.E. Ismail, Abdelbagi M. Jean, M. Ouédraogo, J.T. Roberts, P.A. Timko, M.P. |
| author_facet | Ouédraogo, J.T. Gowda, B.S. Jean, M. Close, T.J. Ehlers, J.D. Hall, A.E. Gillaspie, A.G. Roberts, P.A. Ismail, Abdelbagi M. Bruening, G. Gepts, P. Timko, M.P. Belzile, F.J. |
| author_sort | Ouédraogo, J.T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 242 molecular markers, mostly amplified fragment length polymorphism (AFLP), linked to 17 biological resistance traits, resistance genes, and resistance gene analogs (RGAs) were scored for segregation within the parental and recombinant inbred lines. These data were used in conjunction with the 181 random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), AFLP, and biochemical markers previously mapped to construct an integrated linkage map for cowpea. The new genetic map of cowpea consists of 11 linkage groups (LGs) spanning a total of 2670 cM, with an average distance of 6.43 cM between markers. Astonishingly, a large, contiguous portion of LG1 that had been undetected in previous mapping work was discovered. This region, spanning about 580 cM, is composed entirely of AFLP markers (54 in total). In addition to the construction of a new map, molecular markers associated with various biological resistance and (or) tolerance traits, resistance genes, and RGAs were also placed on the map, including markers for resistance to Striga gesnerioides races 1 and 3, CPMV, CPSMV, B1CMV, SBMV, Fusarium wilt, and root-knot nematodes. These markers will be useful for the development of tools for marker-assisted selection in cowpea breeding, as well as for subsequent map-based cloning of the various resistance genes.Key words: Cowpea, AFLP, RAPD, resistance gene analogs, genetic linkage map. |
| format | Journal Article |
| id | CGSpace166984 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace1669842025-05-14T10:24:01Z An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits Ouédraogo, J.T. Gowda, B.S. Jean, M. Close, T.J. Ehlers, J.D. Hall, A.E. Gillaspie, A.G. Roberts, P.A. Ismail, Abdelbagi M. Bruening, G. Gepts, P. Timko, M.P. Belzile, F.J. An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 242 molecular markers, mostly amplified fragment length polymorphism (AFLP), linked to 17 biological resistance traits, resistance genes, and resistance gene analogs (RGAs) were scored for segregation within the parental and recombinant inbred lines. These data were used in conjunction with the 181 random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), AFLP, and biochemical markers previously mapped to construct an integrated linkage map for cowpea. The new genetic map of cowpea consists of 11 linkage groups (LGs) spanning a total of 2670 cM, with an average distance of 6.43 cM between markers. Astonishingly, a large, contiguous portion of LG1 that had been undetected in previous mapping work was discovered. This region, spanning about 580 cM, is composed entirely of AFLP markers (54 in total). In addition to the construction of a new map, molecular markers associated with various biological resistance and (or) tolerance traits, resistance genes, and RGAs were also placed on the map, including markers for resistance to Striga gesnerioides races 1 and 3, CPMV, CPSMV, B1CMV, SBMV, Fusarium wilt, and root-knot nematodes. These markers will be useful for the development of tools for marker-assisted selection in cowpea breeding, as well as for subsequent map-based cloning of the various resistance genes.Key words: Cowpea, AFLP, RAPD, resistance gene analogs, genetic linkage map. 2002-02-01 2024-12-19T12:56:54Z 2024-12-19T12:56:54Z Journal Article https://hdl.handle.net/10568/166984 en Canadian Science Publishing Ouédraogo, J T; Gowda, B S; Jean, M; Close, T J; Ehlers, J D; Hall, A E; Gillaspie, A G; Roberts, P A; Ismail, A M; Bruening, G; Gepts, P; Timko, M P and Belzile, F J. 2002. An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits. Genome, Volume 45 no. 1 p. 175-188 |
| spellingShingle | Ouédraogo, J.T. Gowda, B.S. Jean, M. Close, T.J. Ehlers, J.D. Hall, A.E. Gillaspie, A.G. Roberts, P.A. Ismail, Abdelbagi M. Bruening, G. Gepts, P. Timko, M.P. Belzile, F.J. An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title | An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title_full | An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title_fullStr | An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title_full_unstemmed | An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title_short | An improved genetic linkage map for cowpea (Vigna unguiculata L.) combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits |
| title_sort | improved genetic linkage map for cowpea vigna unguiculata l combining aflp rflp rapd biochemical markers and biological resistance traits |
| url | https://hdl.handle.net/10568/166984 |
| work_keys_str_mv | AT ouedraogojt animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT gowdabs animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT jeanm animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT closetj animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT ehlersjd animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT hallae animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT gillaspieag animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT robertspa animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT ismailabdelbagim animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT brueningg animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT geptsp animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT timkomp animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT belzilefj animprovedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT ouedraogojt improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT gowdabs improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT jeanm improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT closetj improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT ehlersjd improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT hallae improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT gillaspieag improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT robertspa improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT ismailabdelbagim improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT brueningg improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT geptsp improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT timkomp improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits AT belzilefj improvedgeneticlinkagemapforcowpeavignaunguiculatalcombiningaflprflprapdbiochemicalmarkersandbiologicalresistancetraits |