Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems
Vitamin‐A deficiency (VAD) is a major malnutrition problem in South Asia, where indica rice is the staple food. Indica‐type rice varieties feed more than 2 billion people. Hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitami...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166872 |
| _version_ | 1855534624864731136 |
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| author | Datta, Karabi Baisakh, Niranjan Oliva, Norman Torrizo, Lina Abrigo, Editha Tan, Jing Rai, Mayank Rehana, Sayda Al-Babili, Salim Beyer, Peter Potrykus, Ingo Datta, Swapan K. |
| author_browse | Abrigo, Editha Al-Babili, Salim Baisakh, Niranjan Beyer, Peter Datta, Karabi Datta, Swapan K. Oliva, Norman Potrykus, Ingo Rai, Mayank Rehana, Sayda Tan, Jing Torrizo, Lina |
| author_facet | Datta, Karabi Baisakh, Niranjan Oliva, Norman Torrizo, Lina Abrigo, Editha Tan, Jing Rai, Mayank Rehana, Sayda Al-Babili, Salim Beyer, Peter Potrykus, Ingo Datta, Swapan K. |
| author_sort | Datta, Karabi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Vitamin‐A deficiency (VAD) is a major malnutrition problem in South Asia, where indica rice is the staple food. Indica‐type rice varieties feed more than 2 billion people. Hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitamin A in the endosperm of several indica rice cultivars adapted to diverse ecosystems of different countries. The rice seed‐specific glutelin promoter (Gt‐1 P) was used to drive the expression of phytoene synthase (psy), while lycopene β‐cyclase (lcy) and phytoene desaturase (crtI), fused to the transit peptide sequence of the pea‐Rubisco small subunit, were driven by the constitutive cauliflower mosaic virus promoter (CaMV35S P). Transgenic plants were recovered through selection with either CaMV35S P driven hph (hygromycin phosphotransferase) gene or cestrum yellow leaf curling virus promoter (CMP) driven pmi (phophomannose isomerase) gene. Molecular and biochemical analyses demonstrated stable integration and expression of the transgenes. The yellow colour of the polished rice grain evidenced the carotenoid accumulation in the endosperm. The colour intensity correlated with the estimated carotenoid content by spectrophotometric and HPLC analysis. Carotenoid level in cooked polished seeds was comparable (with minor loss of xanthophylls) to that in non‐cooked seeds of the same transgenic line. The variable segregation pattern in T1 selfing generation indicated single to multiple loci insertion of the transgenes in the genome. This is the first report of using nonantibiotic pmi driven by a novel promoter in generating transgenic indica rice for possible future use in human nutrition. |
| format | Journal Article |
| id | CGSpace166872 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1668722025-05-14T10:24:21Z Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems Datta, Karabi Baisakh, Niranjan Oliva, Norman Torrizo, Lina Abrigo, Editha Tan, Jing Rai, Mayank Rehana, Sayda Al-Babili, Salim Beyer, Peter Potrykus, Ingo Datta, Swapan K. beta carotene biolistics transformation glutelins promoters golden rice mannose mannose-6-phosphate isomerase vitamin a. Vitamin‐A deficiency (VAD) is a major malnutrition problem in South Asia, where indica rice is the staple food. Indica‐type rice varieties feed more than 2 billion people. Hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitamin A in the endosperm of several indica rice cultivars adapted to diverse ecosystems of different countries. The rice seed‐specific glutelin promoter (Gt‐1 P) was used to drive the expression of phytoene synthase (psy), while lycopene β‐cyclase (lcy) and phytoene desaturase (crtI), fused to the transit peptide sequence of the pea‐Rubisco small subunit, were driven by the constitutive cauliflower mosaic virus promoter (CaMV35S P). Transgenic plants were recovered through selection with either CaMV35S P driven hph (hygromycin phosphotransferase) gene or cestrum yellow leaf curling virus promoter (CMP) driven pmi (phophomannose isomerase) gene. Molecular and biochemical analyses demonstrated stable integration and expression of the transgenes. The yellow colour of the polished rice grain evidenced the carotenoid accumulation in the endosperm. The colour intensity correlated with the estimated carotenoid content by spectrophotometric and HPLC analysis. Carotenoid level in cooked polished seeds was comparable (with minor loss of xanthophylls) to that in non‐cooked seeds of the same transgenic line. The variable segregation pattern in T1 selfing generation indicated single to multiple loci insertion of the transgenes in the genome. This is the first report of using nonantibiotic pmi driven by a novel promoter in generating transgenic indica rice for possible future use in human nutrition. 2003-03 2024-12-19T12:56:44Z 2024-12-19T12:56:44Z Journal Article https://hdl.handle.net/10568/166872 en Wiley Datta, Karabi; Baisakh, Niranjan; Oliva, Norman; Torrizo, Lina; Abrigo, Editha; Tan, Jing; Rai, Mayank; Rehana, Sayda; Al‐Babili, Salim; Beyer, Peter; Potrykus, Ingo and Datta, Swapan K. 2003. Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnology Journal, Volume 1 no. 2 p. 81-90 |
| spellingShingle | beta carotene biolistics transformation glutelins promoters golden rice mannose mannose-6-phosphate isomerase vitamin a. Datta, Karabi Baisakh, Niranjan Oliva, Norman Torrizo, Lina Abrigo, Editha Tan, Jing Rai, Mayank Rehana, Sayda Al-Babili, Salim Beyer, Peter Potrykus, Ingo Datta, Swapan K. Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title | Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title_full | Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title_fullStr | Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title_full_unstemmed | Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title_short | Bioengineered ‘golden’ indica rice cultivars with β‐carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| title_sort | bioengineered golden indica rice cultivars with β carotene metabolism in the endosperm with hygromycin and mannose selection systems |
| topic | beta carotene biolistics transformation glutelins promoters golden rice mannose mannose-6-phosphate isomerase vitamin a. |
| url | https://hdl.handle.net/10568/166872 |
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