Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation
In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization o...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166868 |
| _version_ | 1855522069165375488 |
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| author | Tu, Jumin Datta, Karabi Oliva, Norman Zhang, Guoan Xu, Caiguo Khush, Gurdev Singh Zhang, Qifa Datta, Swapan K. |
| author_browse | Datta, Karabi Datta, Swapan K. Khush, Gurdev Singh Oliva, Norman Tu, Jumin Xu, Caiguo Zhang, Guoan Zhang, Qifa |
| author_facet | Tu, Jumin Datta, Karabi Oliva, Norman Zhang, Guoan Xu, Caiguo Khush, Gurdev Singh Zhang, Qifa Datta, Swapan K. |
| author_sort | Tu, Jumin |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential. |
| format | Journal Article |
| id | CGSpace166868 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1668682025-05-14T10:24:02Z Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation Tu, Jumin Datta, Karabi Oliva, Norman Zhang, Guoan Xu, Caiguo Khush, Gurdev Singh Zhang, Qifa Datta, Swapan K. hybrid rice restorer genes genetic markers transformation transgenic rice segregation insect resistance In this study, we have demonstrated that two independent loci are involved in the integration of the insecticidal protein gene cryIAb/cryIAc and selectable marker gene hph in the recipient genome of the elite Chinese CMS restorer line Minghui 63. We have also documented the structural organization of these transgenes in each locus by restriction enzyme digestion and Southern blot analysis. The independent locus integration of different transgenes allowed us to remove the selectable marker gene hph from the gene of interest simply by self‐segregation. Not having the selectable marker gene will enhance the commercial value of our transgenic line TT51‐1, which showed a consistently high level of resistance against repeated infestations of yellow stem borers and natural outbreaks of leaf‐folders, without a reduction in yield potential. 2003-05 2024-12-19T12:56:44Z 2024-12-19T12:56:44Z Journal Article https://hdl.handle.net/10568/166868 en Wiley Tu, Jumin; Datta, Karabi; Oliva, Norman; Zhang, Guoan; Xu, Caiguo; Khush, Gurdev Singh; Zhang, Qifa and Datta, Swapan Kumar. 2003. Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation. Plant Biotechnology Journal, Volume 1 no. 3 p. 155-165 |
| spellingShingle | hybrid rice restorer genes genetic markers transformation transgenic rice segregation insect resistance Tu, Jumin Datta, Karabi Oliva, Norman Zhang, Guoan Xu, Caiguo Khush, Gurdev Singh Zhang, Qifa Datta, Swapan K. Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title_full | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title_fullStr | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title_full_unstemmed | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title_short | Site-independently integrated transgenes in the elite restorer rice line Minghui 63 allow removal of a selectable marker from the gene of interest by self-segregation |
| title_sort | site independently integrated transgenes in the elite restorer rice line minghui 63 allow removal of a selectable marker from the gene of interest by self segregation |
| topic | hybrid rice restorer genes genetic markers transformation transgenic rice segregation insect resistance |
| url | https://hdl.handle.net/10568/166868 |
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