Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice

Phytohormones are well‐known regulators of the symbiotic Rhizobium–legume association in the plant host. The enod40 nodulin gene is associated with the earliest phases of the nodule organogenesis programme in the legume host and modifying its expression resulted in perturbations of nodule developmen...

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Main Authors: Dey, Moul, Complainville, Arnaud, Charon, Celine, Torrizo, Lina, Kondorosi, Adam, Crespi, Martin, Datta, Swapan
Format: Journal Article
Language:Inglés
Published: Wiley 2004
Subjects:
Online Access:https://hdl.handle.net/10568/166814
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author Dey, Moul
Complainville, Arnaud
Charon, Celine
Torrizo, Lina
Kondorosi, Adam
Crespi, Martin
Datta, Swapan
author_browse Charon, Celine
Complainville, Arnaud
Crespi, Martin
Datta, Swapan
Dey, Moul
Kondorosi, Adam
Torrizo, Lina
author_facet Dey, Moul
Complainville, Arnaud
Charon, Celine
Torrizo, Lina
Kondorosi, Adam
Crespi, Martin
Datta, Swapan
author_sort Dey, Moul
collection Repository of Agricultural Research Outputs (CGSpace)
description Phytohormones are well‐known regulators of the symbiotic Rhizobium–legume association in the plant host. The enod40 nodulin gene is associated with the earliest phases of the nodule organogenesis programme in the legume host and modifying its expression resulted in perturbations of nodule development in Medicago truncatula. Therefore in our pursuit to mimic the initial signal transduction steps of legume nodulation in the alien physiological set‐up of a rice plant, we have expressed the Mtenod40 gene in rice. Molecular data confirm the stable integration, inheritance and transcription of the foreign gene in this non‐legume. We have compared the phytohormonal responses of Mtenod40‐overexpressing and control plants in a homologous legume background (M. truncatula) and in the non‐legume rice. An enod40‐mediated root growth response, induced by inhibition of ethylene biosynthesis, was observed in both plants. On the other hand, a significant differential effect of cytokinins was observed only in rice plants. This suggests that ethylene inhibits enod40 action both in legumes and non‐legumes and reinforces that some of the early signal transduction steps of the nodule developmental programme may function in rice.
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spelling CGSpace1668142024-12-19T14:11:58Z Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice Dey, Moul Complainville, Arnaud Charon, Celine Torrizo, Lina Kondorosi, Adam Crespi, Martin Datta, Swapan nodulins genes gene expression growth regulators transgenics hygromycin b promoters cytokinins acc avg ethylene inhibition transgenic rice Phytohormones are well‐known regulators of the symbiotic Rhizobium–legume association in the plant host. The enod40 nodulin gene is associated with the earliest phases of the nodule organogenesis programme in the legume host and modifying its expression resulted in perturbations of nodule development in Medicago truncatula. Therefore in our pursuit to mimic the initial signal transduction steps of legume nodulation in the alien physiological set‐up of a rice plant, we have expressed the Mtenod40 gene in rice. Molecular data confirm the stable integration, inheritance and transcription of the foreign gene in this non‐legume. We have compared the phytohormonal responses of Mtenod40‐overexpressing and control plants in a homologous legume background (M. truncatula) and in the non‐legume rice. An enod40‐mediated root growth response, induced by inhibition of ethylene biosynthesis, was observed in both plants. On the other hand, a significant differential effect of cytokinins was observed only in rice plants. This suggests that ethylene inhibits enod40 action both in legumes and non‐legumes and reinforces that some of the early signal transduction steps of the nodule developmental programme may function in rice. 2004-01 2024-12-19T12:56:42Z 2024-12-19T12:56:42Z Journal Article https://hdl.handle.net/10568/166814 en Wiley Dey, Moul; Complainville, Arnaud; Charon, Celine; Torrizo, Lina; Kondorosi, Adam; Crespi, Martin and Datta, Swapan. 2004. Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice. Physiologia Plantarum, Volume 120 no. 1 p. 132-139
spellingShingle nodulins
genes
gene expression
growth regulators
transgenics
hygromycin b
promoters
cytokinins
acc
avg
ethylene
inhibition
transgenic rice
Dey, Moul
Complainville, Arnaud
Charon, Celine
Torrizo, Lina
Kondorosi, Adam
Crespi, Martin
Datta, Swapan
Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title_full Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title_fullStr Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title_full_unstemmed Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title_short Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice
title_sort phytohormonal responses in enod40 overexpressing plants of medicago truncatula and rice
topic nodulins
genes
gene expression
growth regulators
transgenics
hygromycin b
promoters
cytokinins
acc
avg
ethylene
inhibition
transgenic rice
url https://hdl.handle.net/10568/166814
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