Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice
Cultivation‐independent studies suggest that roots of rice (Oryza sativa) are colonized by a diverse community of nitrogen‐fixing bacteria. Here we report for the first time mRNA‐based profiling of nitrogenase (nifH) genes, to study the impact of lowland‐rice genotypes at the cultivar level on the f...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2005
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166722 |
| _version_ | 1855533828129423360 |
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| author | Knauth, Stefan Hurek, Thomas Brar, Darshan Reinhold-Hurek, Barbara |
| author_browse | Brar, Darshan Hurek, Thomas Knauth, Stefan Reinhold-Hurek, Barbara |
| author_facet | Knauth, Stefan Hurek, Thomas Brar, Darshan Reinhold-Hurek, Barbara |
| author_sort | Knauth, Stefan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cultivation‐independent studies suggest that roots of rice (Oryza sativa) are colonized by a diverse community of nitrogen‐fixing bacteria. Here we report for the first time mRNA‐based profiling of nitrogenase (nifH) genes, to study the impact of lowland‐rice genotypes at the cultivar level on the functional diversity of root‐associated diazotrophs. Root RNA extracts from all plants contained nifH mRNA at levels detectable by reverse transcription polymerase chain reaction (RT‐PCR). Terminal restriction fragment length polymorphism (T‐RFLP) analysis of RT‐PCR products showed only small interplant variations. However, RNA‐ and DNA‐based profiles obtained from the same root extractions differed from each other, suggesting that presence of diazotrophs did not necessarily coincide with active transcription of nif genes. Application of N‐fertilizer at planting had a long‐term effect on the profile of expressed nitrogenase genes. Phylogenetic analysis of a clone library constructed for nifH fragments expressed in wild species of rice roots indicated that active diazotrophs were not related to cultured strains. The composition of active diazotrophic communities was compared for six related cultivars of O. sativa, wild species Oryza brachyantha, and a genetic cross between it and cv. IR56, grown under identical conditions in rice field soil in the Philippines without N‐fertilizer application. Remarkable varietal differences in root associated nifH‐gene expressing communities were detected. This underlines the importance of mRNA‐based approaches to study functional diversity and eventually identify key diazotrophs in a particular environment. |
| format | Journal Article |
| id | CGSpace166722 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1667222025-05-14T10:24:20Z Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice Knauth, Stefan Hurek, Thomas Brar, Darshan Reinhold-Hurek, Barbara cultivars dna libraries genes gene expression genetic markers nitrogen fertilizers nitrogen fixing bacteria nitrogenase polymerase chain reaction restriction fragment length polymorphism rna roots Cultivation‐independent studies suggest that roots of rice (Oryza sativa) are colonized by a diverse community of nitrogen‐fixing bacteria. Here we report for the first time mRNA‐based profiling of nitrogenase (nifH) genes, to study the impact of lowland‐rice genotypes at the cultivar level on the functional diversity of root‐associated diazotrophs. Root RNA extracts from all plants contained nifH mRNA at levels detectable by reverse transcription polymerase chain reaction (RT‐PCR). Terminal restriction fragment length polymorphism (T‐RFLP) analysis of RT‐PCR products showed only small interplant variations. However, RNA‐ and DNA‐based profiles obtained from the same root extractions differed from each other, suggesting that presence of diazotrophs did not necessarily coincide with active transcription of nif genes. Application of N‐fertilizer at planting had a long‐term effect on the profile of expressed nitrogenase genes. Phylogenetic analysis of a clone library constructed for nifH fragments expressed in wild species of rice roots indicated that active diazotrophs were not related to cultured strains. The composition of active diazotrophic communities was compared for six related cultivars of O. sativa, wild species Oryza brachyantha, and a genetic cross between it and cv. IR56, grown under identical conditions in rice field soil in the Philippines without N‐fertilizer application. Remarkable varietal differences in root associated nifH‐gene expressing communities were detected. This underlines the importance of mRNA‐based approaches to study functional diversity and eventually identify key diazotrophs in a particular environment. 2005-11 2024-12-19T12:56:35Z 2024-12-19T12:56:35Z Journal Article https://hdl.handle.net/10568/166722 en Wiley Knauth, Stefan; Hurek, Thomas; Brar, Darshan and Reinhold‐Hurek, Barbara. 2005. Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice. Environmental Microbiology, Volume 7 no. 11 p. 1725-1733 |
| spellingShingle | cultivars dna libraries genes gene expression genetic markers nitrogen fertilizers nitrogen fixing bacteria nitrogenase polymerase chain reaction restriction fragment length polymorphism rna roots Knauth, Stefan Hurek, Thomas Brar, Darshan Reinhold-Hurek, Barbara Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title | Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title_full | Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title_fullStr | Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title_full_unstemmed | Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title_short | Influence of different Oryza cultivars on expression of nifH gene pools in roots of rice |
| title_sort | influence of different oryza cultivars on expression of nifh gene pools in roots of rice |
| topic | cultivars dna libraries genes gene expression genetic markers nitrogen fertilizers nitrogen fixing bacteria nitrogenase polymerase chain reaction restriction fragment length polymorphism rna roots |
| url | https://hdl.handle.net/10568/166722 |
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