Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage
Rice (Oryza sativa), a salt-sensitive species, has considerable genetic variation for salt tolerance within the cultivated gene pool. Two indica rice genotypes, FL478, a recombinant inbred line derived from a population developed for salinity tolerance studies, and IR29, the sensitive parent of the...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166705 |
| _version_ | 1855528867624648704 |
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| author | Walia, Harkamal Wilson, Clyde Condamine, Pascal Liu, Xuan Ismail, Abdelbagi M. Zeng, Linghe Wanamaker, Steve I. Mandal, Jayati Xu, Jin Cui, Xinping Close, Timothy J. |
| author_browse | Close, Timothy J. Condamine, Pascal Cui, Xinping Ismail, Abdelbagi M. Liu, Xuan Mandal, Jayati Walia, Harkamal Wanamaker, Steve I. Wilson, Clyde Xu, Jin Zeng, Linghe |
| author_facet | Walia, Harkamal Wilson, Clyde Condamine, Pascal Liu, Xuan Ismail, Abdelbagi M. Zeng, Linghe Wanamaker, Steve I. Mandal, Jayati Xu, Jin Cui, Xinping Close, Timothy J. |
| author_sort | Walia, Harkamal |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice (Oryza sativa), a salt-sensitive species, has considerable genetic variation for salt tolerance within the cultivated gene pool. Two indica rice genotypes, FL478, a recombinant inbred line derived from a population developed for salinity tolerance studies, and IR29, the sensitive parent of the population, were selected for this study. We used the Affymetrix rice genome array containing 55,515 probe sets to explore the transcriptome of the salt-tolerant and salt-sensitive genotypes under control and salinity-stressed conditions during vegetative growth. Response of the sensitive genotype IR29 is characterized by induction of a relatively large number of probe sets compared to tolerant FL478. Salinity stress induced a number of genes involved in the flavonoid biosynthesis pathway in IR29 but not in FL478. Cell wall-related genes were responsive in both genotypes, suggesting cell wall restructuring is a general adaptive mechanism during salinity stress, although the two genotypes also had some differences. Additionally, the expression of genes mapping to the Saltol region of chromosome 1 were examined in both genotypes. Single-feature polymorphism analysis of expression data revealed that IR29 was the source of the Saltol region in FL478, contrary to expectation. This study provides a genome-wide transcriptional analysis of two well-characterized, genetically related rice genotypes differing in salinity tolerance during a gradually imposed salinity stress under greenhouse conditions. |
| format | Journal Article |
| id | CGSpace166705 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1667052024-12-22T05:44:55Z Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage Walia, Harkamal Wilson, Clyde Condamine, Pascal Liu, Xuan Ismail, Abdelbagi M. Zeng, Linghe Wanamaker, Steve I. Mandal, Jayati Xu, Jin Cui, Xinping Close, Timothy J. biosynthesis cell walls genes gene expression gene mapping genomes genotypes growth inbred lines salinity salt tolerance stress stress response transcription Rice (Oryza sativa), a salt-sensitive species, has considerable genetic variation for salt tolerance within the cultivated gene pool. Two indica rice genotypes, FL478, a recombinant inbred line derived from a population developed for salinity tolerance studies, and IR29, the sensitive parent of the population, were selected for this study. We used the Affymetrix rice genome array containing 55,515 probe sets to explore the transcriptome of the salt-tolerant and salt-sensitive genotypes under control and salinity-stressed conditions during vegetative growth. Response of the sensitive genotype IR29 is characterized by induction of a relatively large number of probe sets compared to tolerant FL478. Salinity stress induced a number of genes involved in the flavonoid biosynthesis pathway in IR29 but not in FL478. Cell wall-related genes were responsive in both genotypes, suggesting cell wall restructuring is a general adaptive mechanism during salinity stress, although the two genotypes also had some differences. Additionally, the expression of genes mapping to the Saltol region of chromosome 1 were examined in both genotypes. Single-feature polymorphism analysis of expression data revealed that IR29 was the source of the Saltol region in FL478, contrary to expectation. This study provides a genome-wide transcriptional analysis of two well-characterized, genetically related rice genotypes differing in salinity tolerance during a gradually imposed salinity stress under greenhouse conditions. 2005-10-01 2024-12-19T12:56:34Z 2024-12-19T12:56:34Z Journal Article https://hdl.handle.net/10568/166705 en Oxford University Press Walia, Harkamal; Wilson, Clyde; Condamine, Pascal; Liu, Xuan; Ismail, Abdelbagi M.; Zeng, Linghe; Wanamaker, Steve I.; Mandal, Jayati; Xu, Jin; Cui, Xinping and Close, Timothy J. 2005. Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. |
| spellingShingle | biosynthesis cell walls genes gene expression gene mapping genomes genotypes growth inbred lines salinity salt tolerance stress stress response transcription Walia, Harkamal Wilson, Clyde Condamine, Pascal Liu, Xuan Ismail, Abdelbagi M. Zeng, Linghe Wanamaker, Steve I. Mandal, Jayati Xu, Jin Cui, Xinping Close, Timothy J. Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title | Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title_full | Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title_fullStr | Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title_full_unstemmed | Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title_short | Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| title_sort | comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage |
| topic | biosynthesis cell walls genes gene expression gene mapping genomes genotypes growth inbred lines salinity salt tolerance stress stress response transcription |
| url | https://hdl.handle.net/10568/166705 |
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