Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars
We have shown the efficacies of translationally fused gene (cry1Ab‐1B) under the control of maize ubiquitin promoter and hybrid Bt gene (cry1A/cry1Ac) driven by rice Actin‐1 promoter against the yellow stem borer (Scirpophaga incertulas Walker, YSB) insect in rice (Oryza sativa L.). The integration,...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166660 |
| _version_ | 1855529381144821760 |
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| author | Ho, N.H. Baisakh, N. Oliva, N. Datta, K. Frutos, R. Datta, S.K. |
| author_browse | Baisakh, N. Datta, K. Datta, S.K. Frutos, R. Ho, N.H. Oliva, N. |
| author_facet | Ho, N.H. Baisakh, N. Oliva, N. Datta, K. Frutos, R. Datta, S.K. |
| author_sort | Ho, N.H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have shown the efficacies of translationally fused gene (cry1Ab‐1B) under the control of maize ubiquitin promoter and hybrid Bt gene (cry1A/cry1Ac) driven by rice Actin‐1 promoter against the yellow stem borer (Scirpophaga incertulas Walker, YSB) insect in rice (Oryza sativa L.). The integration, expression, and functionality of the transgenes were confirmed by Southern, western, and insect bioassay. The bioassay results correlated with the molecular data. The amount of Bt protein (within the range of 0.8–1.3% of the total soluble protein) in the transgenics was sufficient to cause 100% mortality of the neonate larvae of YSB within 1 wk of infestation. The transgenic plants expressing the foreign Bt protein were normal in phenotype with as good seed setting as the nontransgenic control plants. |
| format | Journal Article |
| id | CGSpace166660 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1666602025-12-08T09:54:28Z Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars Ho, N.H. Baisakh, N. Oliva, N. Datta, K. Frutos, R. Datta, S.K. genes gene expression genetic transformation induced resistance pest resistance promoters transgenic ric scirpophaga incertulas We have shown the efficacies of translationally fused gene (cry1Ab‐1B) under the control of maize ubiquitin promoter and hybrid Bt gene (cry1A/cry1Ac) driven by rice Actin‐1 promoter against the yellow stem borer (Scirpophaga incertulas Walker, YSB) insect in rice (Oryza sativa L.). The integration, expression, and functionality of the transgenes were confirmed by Southern, western, and insect bioassay. The bioassay results correlated with the molecular data. The amount of Bt protein (within the range of 0.8–1.3% of the total soluble protein) in the transgenics was sufficient to cause 100% mortality of the neonate larvae of YSB within 1 wk of infestation. The transgenic plants expressing the foreign Bt protein were normal in phenotype with as good seed setting as the nontransgenic control plants. 2006-03 2024-12-19T12:56:30Z 2024-12-19T12:56:30Z Journal Article https://hdl.handle.net/10568/166660 en Wiley Ho, N. H.; Baisakh, N.; Oliva, N.; Datta, K.; Frutos, R. and Datta, S. K. 2006. Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars. Crop Science, Volume 46 no. 2 p. 781-789 |
| spellingShingle | genes gene expression genetic transformation induced resistance pest resistance promoters transgenic ric scirpophaga incertulas Ho, N.H. Baisakh, N. Oliva, N. Datta, K. Frutos, R. Datta, S.K. Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title | Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title_full | Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title_fullStr | Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title_full_unstemmed | Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title_short | Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite Vietnamese rice (Oryza sativa L.) cultivars |
| title_sort | translational fusion hybrid bt genes confer resistance against yellow stem borer in transgenic elite vietnamese rice oryza sativa l cultivars |
| topic | genes gene expression genetic transformation induced resistance pest resistance promoters transgenic ric scirpophaga incertulas |
| url | https://hdl.handle.net/10568/166660 |
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