Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue
We have developed near‐isogenic introgression lines (NIILs) of an elite indica rice cultivar (IR64) with the genes for β‐carotene biosynthesis from dihaploid (DH) derivatives of golden japonica rice (cv. T309). A careful analysis of the DH lines indicated the integration of the genes of interest [ph...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166603 |
| _version_ | 1855538388594065408 |
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| author | Baisakh, Niranjan Rehana, Sayda Rai, Mayank Oliva, Norman Tan, Jing Mackill, David J. Khush, Gurdev S. Datta, Karabi Datta, Swapan K. |
| author_browse | Baisakh, Niranjan Datta, Karabi Datta, Swapan K. Khush, Gurdev S. Mackill, David J. Oliva, Norman Rai, Mayank Rehana, Sayda Tan, Jing |
| author_facet | Baisakh, Niranjan Rehana, Sayda Rai, Mayank Oliva, Norman Tan, Jing Mackill, David J. Khush, Gurdev S. Datta, Karabi Datta, Swapan K. |
| author_sort | Baisakh, Niranjan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have developed near‐isogenic introgression lines (NIILs) of an elite indica rice cultivar (IR64) with the genes for β‐carotene biosynthesis from dihaploid (DH) derivatives of golden japonica rice (cv. T309). A careful analysis of the DH lines indicated the integration of the genes of interest [phytoene synthase (psy) and phytoene desaturase (crtI)] and the selectable marker gene (hygromycin phosphotransferase,hph) in two unlinked loci. During subsequent crossing, progenies could be obtained carrying only the locus withpsyandcrtI, which was segregated independently from the locus containing thehphgene during meiotic segregation. The NIILs (BC2F2) showed maximum similarity with the recurrent parent cultivar IR64. Further, progenies of two NIILs were devoid of any fragments beyond the left or right border, including the chloramphenicol acetyltransferase (cat) antibiotic resistance gene of the transformation vector. Spectrophotometric readings showed the accumulation of up to 1.06 µg total carotenoids, including β‐carotene, in 1 g of the endosperm. The accumulation of β‐carotene was also evident from the clearly visible yellow colour of the polished seeds. |
| format | Journal Article |
| id | CGSpace166603 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1666032024-12-19T14:13:31Z Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue Baisakh, Niranjan Rehana, Sayda Rai, Mayank Oliva, Norman Tan, Jing Mackill, David J. Khush, Gurdev S. Datta, Karabi Datta, Swapan K. beta-carotene carotenoids endosperm genes gene expression gene transfer genetic transformation loci marker genes provitamins transgenic rice golden rice We have developed near‐isogenic introgression lines (NIILs) of an elite indica rice cultivar (IR64) with the genes for β‐carotene biosynthesis from dihaploid (DH) derivatives of golden japonica rice (cv. T309). A careful analysis of the DH lines indicated the integration of the genes of interest [phytoene synthase (psy) and phytoene desaturase (crtI)] and the selectable marker gene (hygromycin phosphotransferase,hph) in two unlinked loci. During subsequent crossing, progenies could be obtained carrying only the locus withpsyandcrtI, which was segregated independently from the locus containing thehphgene during meiotic segregation. The NIILs (BC2F2) showed maximum similarity with the recurrent parent cultivar IR64. Further, progenies of two NIILs were devoid of any fragments beyond the left or right border, including the chloramphenicol acetyltransferase (cat) antibiotic resistance gene of the transformation vector. Spectrophotometric readings showed the accumulation of up to 1.06 µg total carotenoids, including β‐carotene, in 1 g of the endosperm. The accumulation of β‐carotene was also evident from the clearly visible yellow colour of the polished seeds. 2006-07 2024-12-19T12:56:27Z 2024-12-19T12:56:27Z Journal Article https://hdl.handle.net/10568/166603 en Wiley Baisakh, Niranjan; Rehana, Sayda; Rai, Mayank; Oliva, Norman; Tan, Jing; Mackill, David J.; Khush, Gurdev S.; Datta, Karabi and Datta, Swapan K. 2006. Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue. Plant Biotechnology Journal, Volume 4 no. 4 p. 467-475 |
| spellingShingle | beta-carotene carotenoids endosperm genes gene expression gene transfer genetic transformation loci marker genes provitamins transgenic rice golden rice Baisakh, Niranjan Rehana, Sayda Rai, Mayank Oliva, Norman Tan, Jing Mackill, David J. Khush, Gurdev S. Datta, Karabi Datta, Swapan K. Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title | Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title_full | Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title_fullStr | Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title_full_unstemmed | Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title_short | Marker-free transgenic (MFT) near-isogenic introgression lines (NIILs) of golden indica rice (cv. IR64) with accumulation of provitamin A in the endosperm tissue |
| title_sort | marker free transgenic mft near isogenic introgression lines niils of golden indica rice cv ir64 with accumulation of provitamin a in the endosperm tissue |
| topic | beta-carotene carotenoids endosperm genes gene expression gene transfer genetic transformation loci marker genes provitamins transgenic rice golden rice |
| url | https://hdl.handle.net/10568/166603 |
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