A high-throughput genomic tool: diversity array technology complementary for rice genotyping
Diversity array technology (DArTTM) was a genotyping tool characterized gel‐independent and high throughput. The main purpose of present study is to validate DArT for rice (Oryza sativa L.)genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166559 |
| _version_ | 1855539698788728832 |
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| author | Xie, Yong McNally, Kenneth Li, Cheng-Yun Leung, Hei Zhu, Youyong |
| author_browse | Leung, Hei Li, Cheng-Yun McNally, Kenneth Xie, Yong Zhu, Youyong |
| author_facet | Xie, Yong McNally, Kenneth Li, Cheng-Yun Leung, Hei Zhu, Youyong |
| author_sort | Xie, Yong |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Diversity array technology (DArTTM) was a genotyping tool characterized gel‐independent and high throughput. The main purpose of present study is to validate DArT for rice (Oryza sativa L.)genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose gene pool, and optimize this genomic tool in order to evaluate rice germplasm genetic diversity. To achieve this, firstly, a general‐purpose DArT array was developed. Ten representatives from 24 varieties were hybridized with the general‐purpose array to determine the informativeness of the clones printed on the array. The informative 1 152 clones were re‐arrayed on a slide and used to fingerprint 17 of 24 germplasms. Hybridizing targets prepared from the germplasm to be assayed to the DNA array gave DNA fingerprints of germplasms. Raw data were normalized and transformed into binary data, which were then analyzed by using NTSYSpc (Numerical taxonomy system for cluster and ordination analysis, v. 2.02j) software package. The graphically displayed dendrogram derived from the array experimental data was matched with simple sequence repeats genotyping outline and varieties' pedigree deviation of the different varieties. Considering DArT is a sequence‐independent genotyping approach, it will be applied in studies of the genetic diversity and the gene mapping of diverse of organisms, especially for those crops with less‐developed molecular markers.(Managing editor: Ya‐Qin Han) |
| format | Journal Article |
| id | CGSpace166559 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1665592025-05-14T10:24:28Z A high-throughput genomic tool: diversity array technology complementary for rice genotyping Xie, Yong McNally, Kenneth Li, Cheng-Yun Leung, Hei Zhu, Youyong amplified fragment length polymorphism dna microarrays genes gene mapping genetic diversity genetic markers genomes genome analysis genomics genotypes germplasm Diversity array technology (DArTTM) was a genotyping tool characterized gel‐independent and high throughput. The main purpose of present study is to validate DArT for rice (Oryza sativa L.)genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose gene pool, and optimize this genomic tool in order to evaluate rice germplasm genetic diversity. To achieve this, firstly, a general‐purpose DArT array was developed. Ten representatives from 24 varieties were hybridized with the general‐purpose array to determine the informativeness of the clones printed on the array. The informative 1 152 clones were re‐arrayed on a slide and used to fingerprint 17 of 24 germplasms. Hybridizing targets prepared from the germplasm to be assayed to the DNA array gave DNA fingerprints of germplasms. Raw data were normalized and transformed into binary data, which were then analyzed by using NTSYSpc (Numerical taxonomy system for cluster and ordination analysis, v. 2.02j) software package. The graphically displayed dendrogram derived from the array experimental data was matched with simple sequence repeats genotyping outline and varieties' pedigree deviation of the different varieties. Considering DArT is a sequence‐independent genotyping approach, it will be applied in studies of the genetic diversity and the gene mapping of diverse of organisms, especially for those crops with less‐developed molecular markers.(Managing editor: Ya‐Qin Han) 2006-09 2024-12-19T12:56:23Z 2024-12-19T12:56:23Z Journal Article https://hdl.handle.net/10568/166559 en Wiley Xie, Yong; McNally, Kenneth; Li, Cheng‐Yun; Leung, Hei and Zhu, You‐Yong. 2006. A high-throughput genomic tool: diversity array technology complementary for rice genotyping. JIPB, Volume 48 no. 9 p. 1069-1076 |
| spellingShingle | amplified fragment length polymorphism dna microarrays genes gene mapping genetic diversity genetic markers genomes genome analysis genomics genotypes germplasm Xie, Yong McNally, Kenneth Li, Cheng-Yun Leung, Hei Zhu, Youyong A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title | A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title_full | A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title_fullStr | A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title_full_unstemmed | A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title_short | A high-throughput genomic tool: diversity array technology complementary for rice genotyping |
| title_sort | high throughput genomic tool diversity array technology complementary for rice genotyping |
| topic | amplified fragment length polymorphism dna microarrays genes gene mapping genetic diversity genetic markers genomes genome analysis genomics genotypes germplasm |
| url | https://hdl.handle.net/10568/166559 |
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