Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae
One taxonomic characteristic of Bipolaris species is the bipolar germination of conidia, but conidia of Bipolaris oryzae, the causal pathogen of brown spot in rice, are regularly observed to show intercalary germination, a characteristic of Drechslera species. The effect of selection, culture media...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Wiley
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166531 |
| _version_ | 1855533948682108928 |
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| author | Dela Paz, M. A. G. Goodwin, P.H. Raymundo, A.K. Ardales, E.Y. Cruz, C. M. Vera |
| author_browse | Ardales, E.Y. Cruz, C. M. Vera Dela Paz, M. A. G. Goodwin, P.H. Raymundo, A.K. |
| author_facet | Dela Paz, M. A. G. Goodwin, P.H. Raymundo, A.K. Ardales, E.Y. Cruz, C. M. Vera |
| author_sort | Dela Paz, M. A. G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | One taxonomic characteristic of Bipolaris species is the bipolar germination of conidia, but conidia of Bipolaris oryzae, the causal pathogen of brown spot in rice, are regularly observed to show intercalary germination, a characteristic of Drechslera species. The effect of selection, culture media and culture age on type of conidial germination was determined for three brown spot isolates from Cavinti, San Pablo and Palawan in the Philippines, obtained from infected leaves showing typical disease symptoms. Based on the analyses of their ITS1, ITS2 and 5·8S rDNA nucleotide sequences, the local isolates were clearly identified as B. oryzae. Selection for colonies of the three isolates derived from single conidia with either bipolar or intercalary germination had no effect on the number of spores showing bipolar germination in subsequent cultures. Germination on seven different culture media was tested; of these, rabbit food agar and water agar increased the percentage of bipolar germination of conidia, although this varied between isolates. Incubation of the cultures of all three isolates for longer periods prior to harvesting conidia increased the percentage of bipolar‐germinating conidia from c. 40 to c. 90% with 5‐day‐old and 30‐day‐old cultures, respectively. |
| format | Journal Article |
| id | CGSpace166531 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1665312025-05-14T10:39:52Z Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae Dela Paz, M. A. G. Goodwin, P.H. Raymundo, A.K. Ardales, E.Y. Cruz, C. M. Vera conidia intergenic dna phylogenetics plant pathogenic fungi spore germination brown spot bipolaris cochliobolus miyabeanus One taxonomic characteristic of Bipolaris species is the bipolar germination of conidia, but conidia of Bipolaris oryzae, the causal pathogen of brown spot in rice, are regularly observed to show intercalary germination, a characteristic of Drechslera species. The effect of selection, culture media and culture age on type of conidial germination was determined for three brown spot isolates from Cavinti, San Pablo and Palawan in the Philippines, obtained from infected leaves showing typical disease symptoms. Based on the analyses of their ITS1, ITS2 and 5·8S rDNA nucleotide sequences, the local isolates were clearly identified as B. oryzae. Selection for colonies of the three isolates derived from single conidia with either bipolar or intercalary germination had no effect on the number of spores showing bipolar germination in subsequent cultures. Germination on seven different culture media was tested; of these, rabbit food agar and water agar increased the percentage of bipolar germination of conidia, although this varied between isolates. Incubation of the cultures of all three isolates for longer periods prior to harvesting conidia increased the percentage of bipolar‐germinating conidia from c. 40 to c. 90% with 5‐day‐old and 30‐day‐old cultures, respectively. 2006-12 2024-12-19T12:56:22Z 2024-12-19T12:56:22Z Journal Article https://hdl.handle.net/10568/166531 en Wiley Dela Paz, M. A. G.; Goodwin, P. H.; Raymundo, A. K.; Ardales, E. Y. and Cruz, C. M. Vera. 2006. Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae. Plant Pathology, Volume 55 no. 6 p. 756-765 |
| spellingShingle | conidia intergenic dna phylogenetics plant pathogenic fungi spore germination brown spot bipolaris cochliobolus miyabeanus Dela Paz, M. A. G. Goodwin, P.H. Raymundo, A.K. Ardales, E.Y. Cruz, C. M. Vera Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title | Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title_full | Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title_fullStr | Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title_full_unstemmed | Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title_short | Phylogenetic analysis based on ITS sequences and conditions affecting the type of conidial germination of Bipolaris oryzae |
| title_sort | phylogenetic analysis based on its sequences and conditions affecting the type of conidial germination of bipolaris oryzae |
| topic | conidia intergenic dna phylogenetics plant pathogenic fungi spore germination brown spot bipolaris cochliobolus miyabeanus |
| url | https://hdl.handle.net/10568/166531 |
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