Ammonium fluxes into plant roots: Energetics, kinetics and regulation

Ammonium uptake across the plasma membranes of seedling roots of intact rice plants is thermodynamically active at low external concentrations, and consequently, electrogenic uniport is an unlikely mechanism for influx. At higher NH4+ concentrations uptake is passive and electrogenic uniport is a po...

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Main Authors: Glass, Anthony D. M., Erner, Yair, Kronzucker, Herbert J., Schjoerring, Jan K., Siddiqi, M. Yaeesh, Wang, M.-Yuan
Format: Journal Article
Language:Inglés
Published: Wiley 1997
Online Access:https://hdl.handle.net/10568/166504
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author Glass, Anthony D. M.
Erner, Yair
Kronzucker, Herbert J.
Schjoerring, Jan K.
Siddiqi, M. Yaeesh
Wang, M.-Yuan
author_browse Erner, Yair
Glass, Anthony D. M.
Kronzucker, Herbert J.
Schjoerring, Jan K.
Siddiqi, M. Yaeesh
Wang, M.-Yuan
author_facet Glass, Anthony D. M.
Erner, Yair
Kronzucker, Herbert J.
Schjoerring, Jan K.
Siddiqi, M. Yaeesh
Wang, M.-Yuan
author_sort Glass, Anthony D. M.
collection Repository of Agricultural Research Outputs (CGSpace)
description Ammonium uptake across the plasma membranes of seedling roots of intact rice plants is thermodynamically active at low external concentrations, and consequently, electrogenic uniport is an unlikely mechanism for influx. At higher NH4+ concentrations uptake is passive and electrogenic uniport is a possibility. While passive permeation of NH3 is also possible at high external [NH4+], influx measurements at 10 mM NH4+ demonstrated a pH dependence which was inconsistent with significant NH3 permeation. Kinetic studies using 13NH4+ established that influx at low external [H4+] occurred via high affinity transport systems (HATS) in rice and spruce, while at higher [NH4+], influx was mediated by low affinity transport systems (LATS), that showed linear concentration dependence. Ammonium influx via the HATS was shown to be up‐regulated or down‐regulated in response to changes of N status, whereas influx in the LATS was insensitive to N status. The identity or identities of the regulatory signals responsible for controlling influx are discussed.
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spelling CGSpace1665042025-05-14T10:23:53Z Ammonium fluxes into plant roots: Energetics, kinetics and regulation Glass, Anthony D. M. Erner, Yair Kronzucker, Herbert J. Schjoerring, Jan K. Siddiqi, M. Yaeesh Wang, M.-Yuan Ammonium uptake across the plasma membranes of seedling roots of intact rice plants is thermodynamically active at low external concentrations, and consequently, electrogenic uniport is an unlikely mechanism for influx. At higher NH4+ concentrations uptake is passive and electrogenic uniport is a possibility. While passive permeation of NH3 is also possible at high external [NH4+], influx measurements at 10 mM NH4+ demonstrated a pH dependence which was inconsistent with significant NH3 permeation. Kinetic studies using 13NH4+ established that influx at low external [H4+] occurred via high affinity transport systems (HATS) in rice and spruce, while at higher [NH4+], influx was mediated by low affinity transport systems (LATS), that showed linear concentration dependence. Ammonium influx via the HATS was shown to be up‐regulated or down‐regulated in response to changes of N status, whereas influx in the LATS was insensitive to N status. The identity or identities of the regulatory signals responsible for controlling influx are discussed. 1997-01 2024-12-19T12:56:21Z 2024-12-19T12:56:21Z Journal Article https://hdl.handle.net/10568/166504 en Wiley Glass, Anthony D. M.; Erner, Yair; Kronzucker, Herbert J.; Schjoerring, Jan K.; Siddiqi, M. Yaeesh and Wang, M.‐Yuan. 2007. Ammonium fluxes into plant roots: Energetics, kinetics and regulation. J Plant Nutrition andamp; Soil, Volume 160 no. 2 p. 261-268
spellingShingle Glass, Anthony D. M.
Erner, Yair
Kronzucker, Herbert J.
Schjoerring, Jan K.
Siddiqi, M. Yaeesh
Wang, M.-Yuan
Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title_full Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title_fullStr Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title_full_unstemmed Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title_short Ammonium fluxes into plant roots: Energetics, kinetics and regulation
title_sort ammonium fluxes into plant roots energetics kinetics and regulation
url https://hdl.handle.net/10568/166504
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