Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)
A rice genic male‐sterility gene ms‐h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms‐h gene revealed a single nucleotide substitution at the 3′‐splice junction of the 14th intron of the UDP‐glucose pyrophosphorylase 1 (...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2008
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166377 |
| _version_ | 1855524813983973376 |
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| author | Woo, Mi-Ok Ham, Tae-Ho Ji, Hyeon-So Choi, Min-Seon Jiang, Wenzhu Chu, Sang-Ho Piao, Rihua Chin, Joong Hyoun Kim, Jung-A Park, Bong Soo Seo, Hak Soo Jwa, Nam-Soo McCouch, Susan Koh, Hee-Jong |
| author_browse | Chin, Joong Hyoun Choi, Min-Seon Chu, Sang-Ho Ham, Tae-Ho Ji, Hyeon-So Jiang, Wenzhu Jwa, Nam-Soo Kim, Jung-A Koh, Hee-Jong McCouch, Susan Park, Bong Soo Piao, Rihua Seo, Hak Soo Woo, Mi-Ok |
| author_facet | Woo, Mi-Ok Ham, Tae-Ho Ji, Hyeon-So Choi, Min-Seon Jiang, Wenzhu Chu, Sang-Ho Piao, Rihua Chin, Joong Hyoun Kim, Jung-A Park, Bong Soo Seo, Hak Soo Jwa, Nam-Soo McCouch, Susan Koh, Hee-Jong |
| author_sort | Woo, Mi-Ok |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A rice genic male‐sterility gene ms‐h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms‐h gene revealed a single nucleotide substitution at the 3′‐splice junction of the 14th intron of the UDP‐glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1‐RNAi construct in wild‐type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms‐h mutant phenotype, whereas overexpression of UGPase1 in ms‐h mutant plants restored male fertility and the transformants produced T1 seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co‐segregated with the UGPase1 transgene in segregating T1 plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism. |
| format | Journal Article |
| id | CGSpace166377 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1663772025-05-14T10:24:07Z Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) Woo, Mi-Ok Ham, Tae-Ho Ji, Hyeon-So Choi, Min-Seon Jiang, Wenzhu Chu, Sang-Ho Piao, Rihua Chin, Joong Hyoun Kim, Jung-A Park, Bong Soo Seo, Hak Soo Jwa, Nam-Soo McCouch, Susan Koh, Hee-Jong endosperm genes gene expression gene silencing male sterility rna interference A rice genic male‐sterility gene ms‐h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms‐h gene revealed a single nucleotide substitution at the 3′‐splice junction of the 14th intron of the UDP‐glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1‐RNAi construct in wild‐type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms‐h mutant phenotype, whereas overexpression of UGPase1 in ms‐h mutant plants restored male fertility and the transformants produced T1 seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co‐segregated with the UGPase1 transgene in segregating T1 plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism. 2008-04 2024-12-19T12:56:11Z 2024-12-19T12:56:11Z Journal Article https://hdl.handle.net/10568/166377 en Wiley Woo, Mi‐Ok; Ham, Tae‐Ho; Ji, Hyeon‐So; Choi, Min‐Seon; Jiang, Wenzhu; Chu, Sang‐Ho; Piao, Rihua; Chin, Joong‐Hyoun; Kim, Jung‐A; Park, Bong Soo; Seo, Hak Soo; Jwa, Nam‐Soo; McCouch, Susan and Koh, Hee‐Jong. 2008. Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). The Plant Journal, Volume 54 no. 2 p. 190-204 |
| spellingShingle | endosperm genes gene expression gene silencing male sterility rna interference Woo, Mi-Ok Ham, Tae-Ho Ji, Hyeon-So Choi, Min-Seon Jiang, Wenzhu Chu, Sang-Ho Piao, Rihua Chin, Joong Hyoun Kim, Jung-A Park, Bong Soo Seo, Hak Soo Jwa, Nam-Soo McCouch, Susan Koh, Hee-Jong Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title | Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title_full | Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title_fullStr | Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title_full_unstemmed | Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title_short | Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) |
| title_sort | inactivation of the ugpase1 gene causes genic male sterility and endosperm chalkiness in rice oryza sativa l |
| topic | endosperm genes gene expression gene silencing male sterility rna interference |
| url | https://hdl.handle.net/10568/166377 |
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