A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes
Theileria parva is a tick-transmitted protozoan parasite that infects and transforms bovine lymphocytes. We have previously shown that Theileria parva Chitongo is an isolate with a lower virulence than that of T. parva Muguga. Lower virulence appeared to be correlated with a delayed onset of the log...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Society for Microbiology
2012
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| Acceso en línea: | https://hdl.handle.net/10568/16635 |
| _version_ | 1855543047362707456 |
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| author | Tindih, H.S. Geysen, D. Goddeeris, Bruno M. Awino, Elias Dobbelaere, D.A.E. Naessens, Jan |
| author_browse | Awino, Elias Dobbelaere, D.A.E. Geysen, D. Goddeeris, Bruno M. Naessens, Jan Tindih, H.S. |
| author_facet | Tindih, H.S. Geysen, D. Goddeeris, Bruno M. Awino, Elias Dobbelaere, D.A.E. Naessens, Jan |
| author_sort | Tindih, H.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Theileria parva is a tick-transmitted protozoan parasite that infects and transforms bovine lymphocytes. We have previously shown that Theileria parva Chitongo is an isolate with a lower virulence than that of T. parva Muguga. Lower virulence appeared to be correlated with a delayed onset of the logarithmic growth phase of T. parva Chitongo-transformed peripheral blood mononuclear cells after in vitro infection. In the current study, infection experiments with WC1+ γδ T cells revealed that only T. parva Muguga could infect these cells and that no transformed cells could be obtained with T. parva Chitongo sporozoites. Subsequent analysis of the susceptibility of different cell lines and purified populations of lymphocytes to infection and transformation by both isolates showed that T. parva Muguga sporozoites could attach to and infect CD4+, CD8+, and WC1+ T lymphocytes, but T. parva Chitongo sporozoites were observed to bind only to the CD8+ T cell population. Flow cytometry analysis of established, transformed clones confirmed this bias in target cells. T. parva Muguga-transformed clones consisted of different cell surface phenotypes, suggesting that they were derived from either host CD4+, CD8+, or WC1+ T cells. In contrast, all in vitro and in vivo T. parva Chitongo-transformed clones expressed CD8 but not CD4 or WC1, suggesting that the T. parva Chitongo-transformed target cells were exclusively infected CD8+ lymphocytes. Thus, a role of cell tropism in virulence is likely. Since the adhesion molecule p67 is 100% identical between the two strains, a second, high-affinity adhesin that determines target cell specificity appears to exist. |
| format | Journal Article |
| id | CGSpace16635 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace166352025-06-13T04:20:24Z A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes Tindih, H.S. Geysen, D. Goddeeris, Bruno M. Awino, Elias Dobbelaere, D.A.E. Naessens, Jan theileria parva animal diseases Theileria parva is a tick-transmitted protozoan parasite that infects and transforms bovine lymphocytes. We have previously shown that Theileria parva Chitongo is an isolate with a lower virulence than that of T. parva Muguga. Lower virulence appeared to be correlated with a delayed onset of the logarithmic growth phase of T. parva Chitongo-transformed peripheral blood mononuclear cells after in vitro infection. In the current study, infection experiments with WC1+ γδ T cells revealed that only T. parva Muguga could infect these cells and that no transformed cells could be obtained with T. parva Chitongo sporozoites. Subsequent analysis of the susceptibility of different cell lines and purified populations of lymphocytes to infection and transformation by both isolates showed that T. parva Muguga sporozoites could attach to and infect CD4+, CD8+, and WC1+ T lymphocytes, but T. parva Chitongo sporozoites were observed to bind only to the CD8+ T cell population. Flow cytometry analysis of established, transformed clones confirmed this bias in target cells. T. parva Muguga-transformed clones consisted of different cell surface phenotypes, suggesting that they were derived from either host CD4+, CD8+, or WC1+ T cells. In contrast, all in vitro and in vivo T. parva Chitongo-transformed clones expressed CD8 but not CD4 or WC1, suggesting that the T. parva Chitongo-transformed target cells were exclusively infected CD8+ lymphocytes. Thus, a role of cell tropism in virulence is likely. Since the adhesion molecule p67 is 100% identical between the two strains, a second, high-affinity adhesin that determines target cell specificity appears to exist. 2012-03 2012-03-12T10:20:07Z 2012-03-12T10:20:07Z Journal Article https://hdl.handle.net/10568/16635 en Open Access American Society for Microbiology Tindih, H.S., Geysen, D., Goddeeris, B.M., Awino, E., Dobbelaere, D.A.E. and Naessens, J. 2012. A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes. Infection and Immunity. 80(3): 1267-1273. |
| spellingShingle | theileria parva animal diseases Tindih, H.S. Geysen, D. Goddeeris, Bruno M. Awino, Elias Dobbelaere, D.A.E. Naessens, Jan A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title | A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title_full | A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title_fullStr | A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title_full_unstemmed | A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title_short | A Theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| title_sort | theileria parva isolate of low virulence infects a subpopulation of lymphocytes |
| topic | theileria parva animal diseases |
| url | https://hdl.handle.net/10568/16635 |
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