A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice
Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QT...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/166247 |
| _version_ | 1855515554756952064 |
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| author | Manosalva, Patricia M. Davidson, Rebecca M. Liu, Bin Zhu, Xiaoyuan Hulbert, Scot H. Leung, Hei Leach, Jan E. |
| author_browse | Davidson, Rebecca M. Hulbert, Scot H. Leach, Jan E. Leung, Hei Liu, Bin Manosalva, Patricia M. Zhu, Xiaoyuan |
| author_facet | Manosalva, Patricia M. Davidson, Rebecca M. Liu, Bin Zhu, Xiaoyuan Hulbert, Scot H. Leung, Hei Leach, Jan E. |
| author_sort | Manosalva, Patricia M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QTL that colocalized with defense response genes were accumulated by marker-aided selection to develop blast-resistant varieties. One advanced backcross line carrying the major-effect QTL on chromosome (chr) 8, which included a cluster of 12 germin-like protein (OsGLP) gene members, exhibited resistance to rice (Oryza sativa) blast disease over 14 cropping seasons. To determine if OsGLP members contribute to resistance and if the resistance was broad spectrum, a highly conserved portion of the OsGLP coding region was used as an RNA interference trigger to silence a few to all expressed chr 8 OsGLP family members. Challenge with two different fungal pathogens (causal agents of rice blast and sheath blight diseases) revealed that as more chr 8 OsGLP genes were suppressed, disease susceptibility of the plants increased. Of the 12 chr 8 OsGLPs, one clustered subfamily (OsGER4) contributed most to resistance. The similarities of sequence, gene organization, and roles in disease resistance of GLP family members in rice and other cereals, including barley (Hordeum vulgare) and wheat (Triticum aestivum), suggest that resistance contributed by the chr 8 OsGLP is a broad-spectrum, basal mechanism conserved among the Gramineae. Natural selection may have preserved a whole gene family to provide a stepwise, flexible defense response to pathogen invasion. |
| format | Journal Article |
| id | CGSpace166247 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1662472025-12-08T09:54:28Z A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice Manosalva, Patricia M. Davidson, Rebecca M. Liu, Bin Zhu, Xiaoyuan Hulbert, Scot H. Leung, Hei Leach, Jan E. chromosomes defence mechanisms disease resistance fungal diseases genes natural selection nucleotide sequences quantitative trait loci rna interference magnaporthe grisea rhizoctonia solani Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QTL that colocalized with defense response genes were accumulated by marker-aided selection to develop blast-resistant varieties. One advanced backcross line carrying the major-effect QTL on chromosome (chr) 8, which included a cluster of 12 germin-like protein (OsGLP) gene members, exhibited resistance to rice (Oryza sativa) blast disease over 14 cropping seasons. To determine if OsGLP members contribute to resistance and if the resistance was broad spectrum, a highly conserved portion of the OsGLP coding region was used as an RNA interference trigger to silence a few to all expressed chr 8 OsGLP family members. Challenge with two different fungal pathogens (causal agents of rice blast and sheath blight diseases) revealed that as more chr 8 OsGLP genes were suppressed, disease susceptibility of the plants increased. Of the 12 chr 8 OsGLPs, one clustered subfamily (OsGER4) contributed most to resistance. The similarities of sequence, gene organization, and roles in disease resistance of GLP family members in rice and other cereals, including barley (Hordeum vulgare) and wheat (Triticum aestivum), suggest that resistance contributed by the chr 8 OsGLP is a broad-spectrum, basal mechanism conserved among the Gramineae. Natural selection may have preserved a whole gene family to provide a stepwise, flexible defense response to pathogen invasion. 2009-01-06 2024-12-19T12:56:02Z 2024-12-19T12:56:02Z Journal Article https://hdl.handle.net/10568/166247 en Open Access Oxford University Press Manosalva, Patricia M.; Davidson, Rebecca M.; Liu, Bin; Zhu, Xiaoyuan; Hulbert, Scot H.; Leung, Hei and Leach, Jan E. 2009. A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice. |
| spellingShingle | chromosomes defence mechanisms disease resistance fungal diseases genes natural selection nucleotide sequences quantitative trait loci rna interference magnaporthe grisea rhizoctonia solani Manosalva, Patricia M. Davidson, Rebecca M. Liu, Bin Zhu, Xiaoyuan Hulbert, Scot H. Leung, Hei Leach, Jan E. A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title | A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title_full | A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title_fullStr | A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title_full_unstemmed | A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title_short | A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice |
| title_sort | germin like protein gene family functions as a complex quantitative trait locus conferring broad spectrum disease resistance in rice |
| topic | chromosomes defence mechanisms disease resistance fungal diseases genes natural selection nucleotide sequences quantitative trait loci rna interference magnaporthe grisea rhizoctonia solani |
| url | https://hdl.handle.net/10568/166247 |
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