Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa
Protein translation is very sensitive to salt stress and the proteins involved in this process may be an important determinant of salt tolerance. We isolated a rice cDNA clone (OseIF1) from a salt-tolerant indica cultivar (Pokkali) subjected to 150 mm NaCl, the deduced amino acid sequence of which h...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Commonwealth Scientific and Industrial Research Organisation
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166227 |
| _version_ | 1855520608020856832 |
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| author | Rangan, Latha Rout, Anusuya Sudarshan, Medhavi Gregorio, Glenn |
| author_browse | Gregorio, Glenn Rangan, Latha Rout, Anusuya Sudarshan, Medhavi |
| author_facet | Rangan, Latha Rout, Anusuya Sudarshan, Medhavi Gregorio, Glenn |
| author_sort | Rangan, Latha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Protein translation is very sensitive to salt stress and the proteins involved in this process may be an important determinant of salt tolerance. We isolated a rice cDNA clone (OseIF1) from a salt-tolerant indica cultivar (Pokkali) subjected to 150 mm NaCl, the deduced amino acid sequence of which had homology with the Sui1 suppressor locus in Saccharomyces cerevisiaei Hansen. The sequence contains 753 bp with an open-reading frame of 345 bp and shares similarity with the sequences of Sui1 and eIF1 in plants and mammals. Southern analysis indicates that the gene is present in more than a single copy per haploid genome and mapped to chromosome 1 of rice. Expression of the gene was increased by salt stress and also upregulated after exogenous ABA and mannitol treatments, suggesting that its induction is related to the water-deficit effect of high salt. |
| format | Journal Article |
| id | CGSpace166227 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Commonwealth Scientific and Industrial Research Organisation |
| publisherStr | Commonwealth Scientific and Industrial Research Organisation |
| record_format | dspace |
| spelling | CGSpace1662272025-12-08T10:29:22Z Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa Rangan, Latha Rout, Anusuya Sudarshan, Medhavi Gregorio, Glenn abscisic acid amino acid sequences chromosomes dna cloning genes gene expression genetic mapping genomes mannitol salinity salt tolerance sodiul chloride stress response translation water deficit Protein translation is very sensitive to salt stress and the proteins involved in this process may be an important determinant of salt tolerance. We isolated a rice cDNA clone (OseIF1) from a salt-tolerant indica cultivar (Pokkali) subjected to 150 mm NaCl, the deduced amino acid sequence of which had homology with the Sui1 suppressor locus in Saccharomyces cerevisiaei Hansen. The sequence contains 753 bp with an open-reading frame of 345 bp and shares similarity with the sequences of Sui1 and eIF1 in plants and mammals. Southern analysis indicates that the gene is present in more than a single copy per haploid genome and mapped to chromosome 1 of rice. Expression of the gene was increased by salt stress and also upregulated after exogenous ABA and mannitol treatments, suggesting that its induction is related to the water-deficit effect of high salt. 2009 2024-12-19T12:56:01Z 2024-12-19T12:56:01Z Journal Article https://hdl.handle.net/10568/166227 en Commonwealth Scientific and Industrial Research Organisation Rangan, Latha; Rout, Anusuya; Sudarshan, Medhavi and Gregorio, Glenn. 2009. Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa. Functional Plant Biol., Volume 36 no. 5 p. 442 |
| spellingShingle | abscisic acid amino acid sequences chromosomes dna cloning genes gene expression genetic mapping genomes mannitol salinity salt tolerance sodiul chloride stress response translation water deficit Rangan, Latha Rout, Anusuya Sudarshan, Medhavi Gregorio, Glenn Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title | Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title_full | Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title_fullStr | Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title_full_unstemmed | Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title_short | Molecular cloning, expression and mapping of the translational initiation factor elF1 gene in Oryza sativa |
| title_sort | molecular cloning expression and mapping of the translational initiation factor elf1 gene in oryza sativa |
| topic | abscisic acid amino acid sequences chromosomes dna cloning genes gene expression genetic mapping genomes mannitol salinity salt tolerance sodiul chloride stress response translation water deficit |
| url | https://hdl.handle.net/10568/166227 |
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