Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes
Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166192 |
| _version_ | 1855524692004175872 |
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| author | Lee, Sang-Kyu Song, Min-Young Seo, Young-Su Kim, Hye-Kyung Ko, Seho Cao, Pei-Jian Suh, Jung-Pil Yi, Gihwan Roh, Jae-Hwan Lee, Sichul An, Gynheung Hahn, Tae-Ryong Wang, Guo-Liang Ronald, Pamela Jeon, Jong-Seong |
| author_browse | An, Gynheung Cao, Pei-Jian Hahn, Tae-Ryong Jeon, Jong-Seong Kim, Hye-Kyung Ko, Seho Lee, Sang-Kyu Lee, Sichul Roh, Jae-Hwan Ronald, Pamela Seo, Young-Su Song, Min-Young Suh, Jung-Pil Wang, Guo-Liang Yi, Gihwan |
| author_facet | Lee, Sang-Kyu Song, Min-Young Seo, Young-Su Kim, Hye-Kyung Ko, Seho Cao, Pei-Jian Suh, Jung-Pil Yi, Gihwan Roh, Jae-Hwan Lee, Sichul An, Gynheung Hahn, Tae-Ryong Wang, Guo-Liang Ronald, Pamela Jeon, Jong-Seong |
| author_sort | Lee, Sang-Kyu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M. oryzae. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to M. oryzae. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M. oryzae. |
| format | Journal Article |
| id | CGSpace166192 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1661922024-12-22T05:44:52Z Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes Lee, Sang-Kyu Song, Min-Young Seo, Young-Su Kim, Hye-Kyung Ko, Seho Cao, Pei-Jian Suh, Jung-Pil Yi, Gihwan Roh, Jae-Hwan Lee, Sichul An, Gynheung Hahn, Tae-Ryong Wang, Guo-Liang Ronald, Pamela Jeon, Jong-Seong crosses disease resistance fungal diseases genes gene expression genetic transformation genomics leucine nucleotides induced resistance transgenic rice Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice. To understand the molecular basis of Pi5-mediated resistance to M. oryzae, we cloned the resistance (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses of 2014 F2 progeny from a mapping population derived from a cross between IR50, a susceptible rice cultivar, and the RIL260 line carrying Pi5 enabled us to narrow down the Pi5 locus to a 130-kb interval. Sequence analysis of this genomic region identified two candidate genes, Pi5-1 and Pi5-2, which encode proteins carrying three motifs characteristic of R genes: an N-terminal coiled-coil (CC) motif, a nucleotide-binding (NB) domain, and a leucine-rich repeat (LRR) motif. In genetic transformation experiments of a susceptible rice cultivar, neither the Pi5-1 nor the Pi5-2 gene was found to confer resistance to M. oryzae. In contrast, transgenic rice plants expressing both of these genes, generated by crossing transgenic lines carrying each gene individually, conferred Pi5-mediated resistance to M. oryzae. Gene expression analysis revealed that Pi5-1 transcripts accumulate after pathogen challenge, whereas the Pi5-2 gene is constitutively expressed. These results indicate that the presence of these two genes is required for rice Pi5-mediated resistance to M. oryzae. 2009-04-01 2024-12-19T12:55:59Z 2024-12-19T12:55:59Z Journal Article https://hdl.handle.net/10568/166192 en Oxford University Press Lee, Sang-Kyu; Song, Min-Young; Seo, Young-Su; Kim, Hye-Kyung; Ko, Seho; Cao, Pei-Jian; Suh, Jung-Pil; Yi, Gihwan; Roh, Jae-Hwan; Lee, Sichul; An, Gynheung; Hahn, Tae-Ryong; Wang, Guo-Liang; Ronald, Pamela and Jeon, Jong-Seong. 2009. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. |
| spellingShingle | crosses disease resistance fungal diseases genes gene expression genetic transformation genomics leucine nucleotides induced resistance transgenic rice Lee, Sang-Kyu Song, Min-Young Seo, Young-Su Kim, Hye-Kyung Ko, Seho Cao, Pei-Jian Suh, Jung-Pil Yi, Gihwan Roh, Jae-Hwan Lee, Sichul An, Gynheung Hahn, Tae-Ryong Wang, Guo-Liang Ronald, Pamela Jeon, Jong-Seong Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title | Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title_full | Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title_fullStr | Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title_full_unstemmed | Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title_short | Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes |
| title_sort | rice pi5 mediated resistance to magnaporthe oryzae requires the presence of two coiled coil nucleotide binding leucine rich repeat genes |
| topic | crosses disease resistance fungal diseases genes gene expression genetic transformation genomics leucine nucleotides induced resistance transgenic rice |
| url | https://hdl.handle.net/10568/166192 |
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