Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae)
Bacillus thuringiensisBerliner isolates were detected in 57% of 801 samples of rice grain dust, soil, rice field arthropods, and miscellaneous habitats (rice straw compost and mammal faeces) collected at 100 sites in the Philippines. The collection yielded 3950 isolates ofB. thuringiensis(8.7 isolat...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
1998
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166156 |
| _version_ | 1855526653897211904 |
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| author | Theunis, W. Aguda, R.M. Cruz, W.T. Decock, C. Peferoen, M. Lambert, B. Bottrell, D.G. Gould, F.L. Litsinger, J.A. Cohen, M.B. |
| author_browse | Aguda, R.M. Bottrell, D.G. Cohen, M.B. Cruz, W.T. Decock, C. Gould, F.L. Lambert, B. Litsinger, J.A. Peferoen, M. Theunis, W. |
| author_facet | Theunis, W. Aguda, R.M. Cruz, W.T. Decock, C. Peferoen, M. Lambert, B. Bottrell, D.G. Gould, F.L. Litsinger, J.A. Cohen, M.B. |
| author_sort | Theunis, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacillus thuringiensisBerliner isolates were detected in 57% of 801 samples of rice grain dust, soil, rice field arthropods, and miscellaneous habitats (rice straw compost and mammal faeces) collected at 100 sites in the Philippines. The collection yielded 3950 isolates ofB. thuringiensis(8.7 isolates/positive sample). Grain dust from rice mills was the richest source (63% of the samples were positive, with 10.2 isolates/positive sample), followed by rice field arthropods, soil, and miscellaneous habitats. Polyclonal antibodies to six δ-endotoxin groups (Cry1A, Cry1B, Cry1C, Cry1D, Cry1E, and Cry3A) were used in enzyme-linked immunosorbent assays (ELISA) to characterize the toxins produced by each isolate. Subsamples of isolates representing the diversity of isolate sources and δ-endotoxin profiles were bioassayed against the yellow stem borer,Scirpophaga incertulas(Walker) and striped stem borer,Chilo suppressalis(Walker). Eighteen isolates highly toxic to both species were selected for characterization of δ-endotoxin genes by polymerase chain reaction (PCR) with primers specific to 14 genes or gene subfamilies, and Western blotting with Cry2A antibodies. At least two novel δ-endotoxin genes, related tocry1Bandcry1F, were detected by DNA sequencing of PCR products. |
| format | Journal Article |
| id | CGSpace166156 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace1661562025-05-14T10:39:43Z Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) Theunis, W. Aguda, R.M. Cruz, W.T. Decock, C. Peferoen, M. Lambert, B. Bottrell, D.G. Gould, F.L. Litsinger, J.A. Cohen, M.B. bacillus thuringiensis habitats distribution endotoxins toxicity scirpophaga incertulas chilo suppressalis stem borers insect control philippines Bacillus thuringiensisBerliner isolates were detected in 57% of 801 samples of rice grain dust, soil, rice field arthropods, and miscellaneous habitats (rice straw compost and mammal faeces) collected at 100 sites in the Philippines. The collection yielded 3950 isolates ofB. thuringiensis(8.7 isolates/positive sample). Grain dust from rice mills was the richest source (63% of the samples were positive, with 10.2 isolates/positive sample), followed by rice field arthropods, soil, and miscellaneous habitats. Polyclonal antibodies to six δ-endotoxin groups (Cry1A, Cry1B, Cry1C, Cry1D, Cry1E, and Cry3A) were used in enzyme-linked immunosorbent assays (ELISA) to characterize the toxins produced by each isolate. Subsamples of isolates representing the diversity of isolate sources and δ-endotoxin profiles were bioassayed against the yellow stem borer,Scirpophaga incertulas(Walker) and striped stem borer,Chilo suppressalis(Walker). Eighteen isolates highly toxic to both species were selected for characterization of δ-endotoxin genes by polymerase chain reaction (PCR) with primers specific to 14 genes or gene subfamilies, and Western blotting with Cry2A antibodies. At least two novel δ-endotoxin genes, related tocry1Bandcry1F, were detected by DNA sequencing of PCR products. 1998-06 2024-12-19T12:55:55Z 2024-12-19T12:55:55Z Journal Article https://hdl.handle.net/10568/166156 en Cambridge University Press Theunis, W.; Aguda, R.M.; Cruz, W.T.; Decock, C.; Peferoen, M.; Lambert, B.; Bottrell, D.G.; Gould, F.L.; Litsinger, J.A. and Cohen, M.B. 1998. Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae). Bull. Entomol. Res., Volume 88 no. 3 p. 335-342 |
| spellingShingle | bacillus thuringiensis habitats distribution endotoxins toxicity scirpophaga incertulas chilo suppressalis stem borers insect control philippines Theunis, W. Aguda, R.M. Cruz, W.T. Decock, C. Peferoen, M. Lambert, B. Bottrell, D.G. Gould, F.L. Litsinger, J.A. Cohen, M.B. Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title | Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title_full | Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title_fullStr | Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title_full_unstemmed | Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title_short | Bacillus thuringiensis isolates from the Philippines: habitat distribution, delta-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) |
| title_sort | bacillus thuringiensis isolates from the philippines habitat distribution delta endotoxin diversity and toxicity to rice stem borers lepidoptera pyralidae |
| topic | bacillus thuringiensis habitats distribution endotoxins toxicity scirpophaga incertulas chilo suppressalis stem borers insect control philippines |
| url | https://hdl.handle.net/10568/166156 |
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