Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice
In addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been explored as an agrono...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2010
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/166077 |
| _version_ | 1855514859856199680 |
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| author | Qiao, Yongli Lee, Song-I Piao, Rihua Jiang, Wenzhu Ham, Tae-Ho Chin, Joong Hyoun Piao, Zhongze Han, Longzhi Kang, Si-Yong Koh, Hee-Jong |
| author_browse | Chin, Joong Hyoun Ham, Tae-Ho Han, Longzhi Jiang, Wenzhu Kang, Si-Yong Koh, Hee-Jong Lee, Song-I Piao, Rihua Piao, Zhongze Qiao, Yongli |
| author_facet | Qiao, Yongli Lee, Song-I Piao, Rihua Jiang, Wenzhu Ham, Tae-Ho Chin, Joong Hyoun Piao, Zhongze Han, Longzhi Kang, Si-Yong Koh, Hee-Jong |
| author_sort | Qiao, Yongli |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been explored as an agronomical trait in breeding and genetics studies. In the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of Oryza sativa ssp. japonica cultivar Hwacheong with MNU. The innermost endosperm of the flo(a) mutant exhibited floury characteristics while the outer layer of the endosperm appeared normal. Starch granules in the flo(a) mutant formed a loosely-packed crystalline structure and X-ray diffraction revealed that the overall crystallinity of the starch was decreased compared to wild-type. The FLO(a) gene was isolated via a map-based cloning approach and predicted to encode the tetratricopeptide repeat domaincontaining protein, OsTPR. Three mutant alleles contain a nucleotide substitution that generated one stop codon or one splice site, respectively, which presumably disrupts the interaction of the functionally conserved TPR motifs. Taken together, our map-based cloning approach pinpointed an OsTPR as a strong candidate of FLO(a), and the proteins that contain TPR motifs might play a significant role in rice starch biosynthetic pathways. |
| format | Journal Article |
| id | CGSpace166077 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1660772025-05-14T10:23:50Z Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice Qiao, Yongli Lee, Song-I Piao, Rihua Jiang, Wenzhu Ham, Tae-Ho Chin, Joong Hyoun Piao, Zhongze Han, Longzhi Kang, Si-Yong Koh, Hee-Jong alleles codons endosperm genes gene mapping mutants starch granules In addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been explored as an agronomical trait in breeding and genetics studies. In the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of Oryza sativa ssp. japonica cultivar Hwacheong with MNU. The innermost endosperm of the flo(a) mutant exhibited floury characteristics while the outer layer of the endosperm appeared normal. Starch granules in the flo(a) mutant formed a loosely-packed crystalline structure and X-ray diffraction revealed that the overall crystallinity of the starch was decreased compared to wild-type. The FLO(a) gene was isolated via a map-based cloning approach and predicted to encode the tetratricopeptide repeat domaincontaining protein, OsTPR. Three mutant alleles contain a nucleotide substitution that generated one stop codon or one splice site, respectively, which presumably disrupts the interaction of the functionally conserved TPR motifs. Taken together, our map-based cloning approach pinpointed an OsTPR as a strong candidate of FLO(a), and the proteins that contain TPR motifs might play a significant role in rice starch biosynthetic pathways. 2010-02 2024-12-19T12:55:48Z 2024-12-19T12:55:48Z Journal Article https://hdl.handle.net/10568/166077 en Open Access Elsevier Qiao, Yongli; Lee, Song-I; Piao, Rihua; Jiang, Wenzhu; Ham, Tae-Ho; Chin, Joong-Hyoun; Piao, Zhongze; Han, Longzhi; Kang, Si-Yong and Koh, Hee-Jong. 2010. Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice. Molecules and Cells, Volume 29 no. 2 p. 167-174 |
| spellingShingle | alleles codons endosperm genes gene mapping mutants starch granules Qiao, Yongli Lee, Song-I Piao, Rihua Jiang, Wenzhu Ham, Tae-Ho Chin, Joong Hyoun Piao, Zhongze Han, Longzhi Kang, Si-Yong Koh, Hee-Jong Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title | Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title_full | Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title_fullStr | Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title_full_unstemmed | Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title_short | Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice |
| title_sort | fine mapping and candidate gene analysis of the floury endosperm gene flo a in rice |
| topic | alleles codons endosperm genes gene mapping mutants starch granules |
| url | https://hdl.handle.net/10568/166077 |
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