Characterizing the Saltol quantitative trait locus for salinity tolerance in rice

This study characterized Pokkali-derived quantitative trait loci (QTLs) for seedling stage salinity tolerance in preparation for use in marker-assisted breeding. An analysis of 100 SSR markers on 140 IR29/Pokkali recombinant inbred lines (RILs) confirmed the location of the Saltol QTL on chromosome...

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Main Authors: Thomson, Michael J., de Ocampo, Marjorie, Egdane, James, Rahman, M. Akhlasur, Sajise, Andres Godwin, Adorada, Dante L., Tumimbang-Raiz, Ellen, Blumwald, Eduardo, Seraj, Zeba I., Singh, Rakesh K., Gregorio, Glenn B., Ismail, Abdelbagi M.
Format: Journal Article
Language:Inglés
Published: Springer 2010
Subjects:
Online Access:https://hdl.handle.net/10568/166006
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author Thomson, Michael J.
de Ocampo, Marjorie
Egdane, James
Rahman, M. Akhlasur
Sajise, Andres Godwin
Adorada, Dante L.
Tumimbang-Raiz, Ellen
Blumwald, Eduardo
Seraj, Zeba I.
Singh, Rakesh K.
Gregorio, Glenn B.
Ismail, Abdelbagi M.
author_browse Adorada, Dante L.
Blumwald, Eduardo
Egdane, James
Gregorio, Glenn B.
Ismail, Abdelbagi M.
Rahman, M. Akhlasur
Sajise, Andres Godwin
Seraj, Zeba I.
Singh, Rakesh K.
Thomson, Michael J.
Tumimbang-Raiz, Ellen
de Ocampo, Marjorie
author_facet Thomson, Michael J.
de Ocampo, Marjorie
Egdane, James
Rahman, M. Akhlasur
Sajise, Andres Godwin
Adorada, Dante L.
Tumimbang-Raiz, Ellen
Blumwald, Eduardo
Seraj, Zeba I.
Singh, Rakesh K.
Gregorio, Glenn B.
Ismail, Abdelbagi M.
author_sort Thomson, Michael J.
collection Repository of Agricultural Research Outputs (CGSpace)
description This study characterized Pokkali-derived quantitative trait loci (QTLs) for seedling stage salinity tolerance in preparation for use in marker-assisted breeding. An analysis of 100 SSR markers on 140 IR29/Pokkali recombinant inbred lines (RILs) confirmed the location of the Saltol QTL on chromosome 1 and identified additional QTLs associated with tolerance. Analysis of a series of backcross lines and near-isogenic lines (NILs) developed to better characterize the effect of the Saltol locus revealed that Saltol mainly acted to control shoot Na+/K+ homeostasis. Multiple QTLs were required to acquire a high level of tolerance. Unexpectedly, multiple Pokkali alleles at Saltol were detected within the RIL population and between backcross lines, and representative lines were compared with seven Pokkali accessions to better characterize this allelic variation. Thus, while the Saltol locus presents a complex scenario, it provides an opportunity for marker-assisted backcrossing to improve salt tolerance of popular varieties followed by targeting multiple loci through QTL pyramiding for areas with higher salt stress.
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spelling CGSpace1660062024-12-19T14:12:31Z Characterizing the Saltol quantitative trait locus for salinity tolerance in rice Thomson, Michael J. de Ocampo, Marjorie Egdane, James Rahman, M. Akhlasur Sajise, Andres Godwin Adorada, Dante L. Tumimbang-Raiz, Ellen Blumwald, Eduardo Seraj, Zeba I. Singh, Rakesh K. Gregorio, Glenn B. Ismail, Abdelbagi M. alleles chromosomes genetic variation homeostasis inbred lines near-isogenic lines quantitative trait loci salinity salt tolerance simple sequence repeats This study characterized Pokkali-derived quantitative trait loci (QTLs) for seedling stage salinity tolerance in preparation for use in marker-assisted breeding. An analysis of 100 SSR markers on 140 IR29/Pokkali recombinant inbred lines (RILs) confirmed the location of the Saltol QTL on chromosome 1 and identified additional QTLs associated with tolerance. Analysis of a series of backcross lines and near-isogenic lines (NILs) developed to better characterize the effect of the Saltol locus revealed that Saltol mainly acted to control shoot Na+/K+ homeostasis. Multiple QTLs were required to acquire a high level of tolerance. Unexpectedly, multiple Pokkali alleles at Saltol were detected within the RIL population and between backcross lines, and representative lines were compared with seven Pokkali accessions to better characterize this allelic variation. Thus, while the Saltol locus presents a complex scenario, it provides an opportunity for marker-assisted backcrossing to improve salt tolerance of popular varieties followed by targeting multiple loci through QTL pyramiding for areas with higher salt stress. 2010-09 2024-12-19T12:55:45Z 2024-12-19T12:55:45Z Journal Article https://hdl.handle.net/10568/166006 en Open Access Springer Thomson, Michael J.; de Ocampo, Marjorie; Egdane, James; Rahman, M. Akhlasur; Sajise, Andres Godwin; Adorada, Dante L.; Tumimbang-Raiz, Ellen; Blumwald, Eduardo; Seraj, Zeba I.; Singh, Rakesh K.; Gregorio, Glenn B. and Ismail, Abdelbagi M. 2010. Characterizing the Saltol quantitative trait locus for salinity tolerance in rice. Rice, Volume 3 no. 2-3 p. 148-160
spellingShingle alleles
chromosomes
genetic variation
homeostasis
inbred lines
near-isogenic lines
quantitative trait loci
salinity
salt tolerance
simple sequence repeats
Thomson, Michael J.
de Ocampo, Marjorie
Egdane, James
Rahman, M. Akhlasur
Sajise, Andres Godwin
Adorada, Dante L.
Tumimbang-Raiz, Ellen
Blumwald, Eduardo
Seraj, Zeba I.
Singh, Rakesh K.
Gregorio, Glenn B.
Ismail, Abdelbagi M.
Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title_full Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title_fullStr Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title_full_unstemmed Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title_short Characterizing the Saltol quantitative trait locus for salinity tolerance in rice
title_sort characterizing the saltol quantitative trait locus for salinity tolerance in rice
topic alleles
chromosomes
genetic variation
homeostasis
inbred lines
near-isogenic lines
quantitative trait loci
salinity
salt tolerance
simple sequence repeats
url https://hdl.handle.net/10568/166006
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