Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.)
With 3 figuresGenetic analysis of F2 and backcross populations of an induced temperature‐sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymor...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Wiley
2012
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| Online Access: | https://hdl.handle.net/10568/165861 |
| _version_ | 1855542775577051136 |
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| author | Hussain, Appibhai J. Ali, Jauhar Siddiq, Ebrahimali A. Gupta, Vidya S. Reddy, Umesh K. Ranjekar, Prabhakar K. |
| author_browse | Ali, Jauhar Gupta, Vidya S. Hussain, Appibhai J. Ranjekar, Prabhakar K. Reddy, Umesh K. Siddiq, Ebrahimali A. |
| author_facet | Hussain, Appibhai J. Ali, Jauhar Siddiq, Ebrahimali A. Gupta, Vidya S. Reddy, Umesh K. Ranjekar, Prabhakar K. |
| author_sort | Hussain, Appibhai J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | With 3 figuresGenetic analysis of F2 and backcross populations of an induced temperature‐sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymorphic randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers through bulked segregant analysis. The RAPD primers UBC 345830, UBC 313927, microsatellites RM224 and RM21 produced putative markers, which differentiate parents and bulks from sterile parent and sterile bulks. The RAPD analysis of individual F2 plants with the primer UBC345830 showed perfect marker–phenotype cosegregation. The 830‐bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33 cM, was eluted and cloned, and sequence information was used for designing sequence‐characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8 cM. TGMS locus was mapped onto chromosome 11 using RM21 and RM224, flanking it at a distance of 4.3 and 3.0 cM, respectively. The DNA markers tightly linked to TGMS gene (tms8) in F 61 can be cost effectively used for marker‐assisted selection of TGMS trait. |
| format | Journal Article |
| id | CGSpace165861 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1658612024-12-19T14:13:48Z Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) Hussain, Appibhai J. Ali, Jauhar Siddiq, Ebrahimali A. Gupta, Vidya S. Reddy, Umesh K. Ranjekar, Prabhakar K. backcrossing dna genes genetic analysis genetic distance genetic markers male sterility microsatellites molecular genetics mutants mutations plant breeding pollen rapd recessive genes selection simple sequence repeats sterility With 3 figuresGenetic analysis of F2 and backcross populations of an induced temperature‐sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymorphic randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers through bulked segregant analysis. The RAPD primers UBC 345830, UBC 313927, microsatellites RM224 and RM21 produced putative markers, which differentiate parents and bulks from sterile parent and sterile bulks. The RAPD analysis of individual F2 plants with the primer UBC345830 showed perfect marker–phenotype cosegregation. The 830‐bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33 cM, was eluted and cloned, and sequence information was used for designing sequence‐characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8 cM. TGMS locus was mapped onto chromosome 11 using RM21 and RM224, flanking it at a distance of 4.3 and 3.0 cM, respectively. The DNA markers tightly linked to TGMS gene (tms8) in F 61 can be cost effectively used for marker‐assisted selection of TGMS trait. 2012-02 2024-12-19T12:55:33Z 2024-12-19T12:55:33Z Journal Article https://hdl.handle.net/10568/165861 en Wiley Hussain, Appibhai J.; Ali, Jauhar; Siddiq, Ebrahimali A.; Gupta, Vidya S.; Reddy, Umesh K. and Ranjekar, Prabhakar K. 2012. Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.). Plant Breeding, Volume 131 no. 1 p. 42-47 |
| spellingShingle | backcrossing dna genes genetic analysis genetic distance genetic markers male sterility microsatellites molecular genetics mutants mutations plant breeding pollen rapd recessive genes selection simple sequence repeats sterility Hussain, Appibhai J. Ali, Jauhar Siddiq, Ebrahimali A. Gupta, Vidya S. Reddy, Umesh K. Ranjekar, Prabhakar K. Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title | Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title_full | Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title_fullStr | Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title_full_unstemmed | Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title_short | Mapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.) |
| title_sort | mapping of tms8 gene for temperature sensitive genic male sterility tgms in rice oryza sativa l |
| topic | backcrossing dna genes genetic analysis genetic distance genetic markers male sterility microsatellites molecular genetics mutants mutations plant breeding pollen rapd recessive genes selection simple sequence repeats sterility |
| url | https://hdl.handle.net/10568/165861 |
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