Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.)
Mesocotyl elongation is an important trait for seedling emergence in direct-seeding cultivation in rice. In this study, a backcross inbred line (BIL) population from a cross between Kasalath and Nipponbare was employed to map quantitative trait loci (QTLs) for mesocotyl elongation. A total of 5 QTLs...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Springer
2012
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| Acceso en línea: | https://hdl.handle.net/10568/165762 |
| _version_ | 1855526614193930240 |
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| author | Lee, Hyun-Sook Sasaki, Kazuhiro Higashitani, Atsushi Ahn, Sang-Nag Sato, Tadashi |
| author_browse | Ahn, Sang-Nag Higashitani, Atsushi Lee, Hyun-Sook Sasaki, Kazuhiro Sato, Tadashi |
| author_facet | Lee, Hyun-Sook Sasaki, Kazuhiro Higashitani, Atsushi Ahn, Sang-Nag Sato, Tadashi |
| author_sort | Lee, Hyun-Sook |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mesocotyl elongation is an important trait for seedling emergence in direct-seeding cultivation in rice. In this study, a backcross inbred line (BIL) population from a cross between Kasalath and Nipponbare was employed to map quantitative trait loci (QTLs) for mesocotyl elongation. A total of 5 QTLs for mesocotyl length were identified on chromosomes 1, 3, 7, 9, and 12 in 2 independent experiments. At all QTL, the Kasalath alleles contributed to an increase in mesocotyl length. Two QTLs (qMel-1 and qMel-3) on chromosomes 1 and 3 were consistently detected in both experiments. To fine map the QTLs, a cross was made between 2 chromosome segment substitution lines (CSSL-6 and CSSL-15), each harboring the Kasalath allele across the qMel-1 and qMel-3 regions, and an F-2:3 population was developed. A two-way ANOVA indicated that no epistatic interaction was detected between the 2 QTLs in the F-2 population (P = 0.31). Moreover, analysis of two F-3 near-isogenic lines (NILs) derived from the same cross, indicated that the 2 QTLs act additively in distinct or complementary pathways in controlling mesocotyl elongation. Substitution mapping indicated that the qMel-1 QTL was located between the 2 SSR markers RM5448 and RM5310, which are 3,799-kb apart, and that the qMel-3 QTL was located between the 2 SSR markers RM3513 and RM1238, which are 6,964-kb apart. To our knowledge, this is the first report to fine-map QTLs for mesocotyl elongation and to analyze their interaction |
| format | Journal Article |
| id | CGSpace165762 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1657622024-12-19T14:12:42Z Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) Lee, Hyun-Sook Sasaki, Kazuhiro Higashitani, Atsushi Ahn, Sang-Nag Sato, Tadashi alleles chromosome mapping chromosomes genetic maps genetic markers mesocotyls quantitative trait loci simple sequence repeats Mesocotyl elongation is an important trait for seedling emergence in direct-seeding cultivation in rice. In this study, a backcross inbred line (BIL) population from a cross between Kasalath and Nipponbare was employed to map quantitative trait loci (QTLs) for mesocotyl elongation. A total of 5 QTLs for mesocotyl length were identified on chromosomes 1, 3, 7, 9, and 12 in 2 independent experiments. At all QTL, the Kasalath alleles contributed to an increase in mesocotyl length. Two QTLs (qMel-1 and qMel-3) on chromosomes 1 and 3 were consistently detected in both experiments. To fine map the QTLs, a cross was made between 2 chromosome segment substitution lines (CSSL-6 and CSSL-15), each harboring the Kasalath allele across the qMel-1 and qMel-3 regions, and an F-2:3 population was developed. A two-way ANOVA indicated that no epistatic interaction was detected between the 2 QTLs in the F-2 population (P = 0.31). Moreover, analysis of two F-3 near-isogenic lines (NILs) derived from the same cross, indicated that the 2 QTLs act additively in distinct or complementary pathways in controlling mesocotyl elongation. Substitution mapping indicated that the qMel-1 QTL was located between the 2 SSR markers RM5448 and RM5310, which are 3,799-kb apart, and that the qMel-3 QTL was located between the 2 SSR markers RM3513 and RM1238, which are 6,964-kb apart. To our knowledge, this is the first report to fine-map QTLs for mesocotyl elongation and to analyze their interaction 2012-12 2024-12-19T12:55:26Z 2024-12-19T12:55:26Z Journal Article https://hdl.handle.net/10568/165762 en Open Access Springer Lee, Hyun-Sook; Sasaki, Kazuhiro; Higashitani, Atsushi; Ahn, Sang-Nag and Sato, Tadashi. 2012. Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.). Rice, Volume 5, no. 1 |
| spellingShingle | alleles chromosome mapping chromosomes genetic maps genetic markers mesocotyls quantitative trait loci simple sequence repeats Lee, Hyun-Sook Sasaki, Kazuhiro Higashitani, Atsushi Ahn, Sang-Nag Sato, Tadashi Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title | Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title_full | Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title_fullStr | Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title_full_unstemmed | Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title_short | Mapping and characterization of quantitative trait loci for mesocotyl elongation in rice (Oryza sativa L.) |
| title_sort | mapping and characterization of quantitative trait loci for mesocotyl elongation in rice oryza sativa l |
| topic | alleles chromosome mapping chromosomes genetic maps genetic markers mesocotyls quantitative trait loci simple sequence repeats |
| url | https://hdl.handle.net/10568/165762 |
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