Evaluating methods for isolating total rna and predicting the success of sequencing phylogenetically diverse plant transcriptomes

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combin...

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Bibliographic Details

Main Authors:	Johnson, Marc T. J., Carpenter, Eric J., Tian, Zhijian, Bruskiewich, Richard, Burris, Jason N., Carrigan, Charlotte T., Chase, Mark W., Clarke, Neil D., Covshoff, Sarah, dePamphilis, Claude W., Edger, Patrick P., Goh, Falicia, Graham, Sean, Greiner, Stephan, Hibberd, Julian M., Jordon-Thaden, Ingrid, Kutchan, Toni M., Leebens-Mack, James, Melkonian, Michael, Miles, Nicholas, Myburg, Henrietta, Patterson, Jordan, Pires, J. Chris, Ralph, Paula, Rolf, Megan, Sage, Rowan F., Soltis, Douglas, Soltis, Pamela, Stevenson, Dennis, Stewart, C. Neal, Surek, Barbara, Thomsen, Christina J. M., Villarreal, Juan Carlos, Wu, Xiaolei, Zhang, Yong, Deyholos, Michael K., Wong, Gane Ka-Shu
Format:	Journal Article
Language:	Inglés
Published:	Public Library of Science 2012
Subjects:	gene expression genes genetic variation nucleotide sequences phylogeny rna transcriptomes transcriptomics
Online Access:	https://hdl.handle.net/10568/165718