Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid

Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine‐grain rice hybrid released and cultivated extensiv...

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Main Authors: Gouda, Patil K., Saikumar, Surapaneni, Varma, Chejerla M. K., Nagesh, Kancharla, Thippeswamy, Sanka, Shenoy, Vinay, Ramesha, Mugalodim S., Shashidhar, Halagappa E.
Format: Journal Article
Language:Inglés
Published: Wiley 2013
Subjects:
Online Access:https://hdl.handle.net/10568/165715
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author Gouda, Patil K.
Saikumar, Surapaneni
Varma, Chejerla M. K.
Nagesh, Kancharla
Thippeswamy, Sanka
Shenoy, Vinay
Ramesha, Mugalodim S.
Shashidhar, Halagappa E.
author_browse Gouda, Patil K.
Nagesh, Kancharla
Ramesha, Mugalodim S.
Saikumar, Surapaneni
Shashidhar, Halagappa E.
Shenoy, Vinay
Thippeswamy, Sanka
Varma, Chejerla M. K.
author_facet Gouda, Patil K.
Saikumar, Surapaneni
Varma, Chejerla M. K.
Nagesh, Kancharla
Thippeswamy, Sanka
Shenoy, Vinay
Ramesha, Mugalodim S.
Shashidhar, Halagappa E.
author_sort Gouda, Patil K.
collection Repository of Agricultural Research Outputs (CGSpace)
description Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine‐grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, Pusa 6A and PRR78, are highly susceptible to blast. CO39 pyramid carrying two dominant, broad‐spectrum blast‐resistance genes, viz. Pi‐1 and Piz‐5, used as a donor parent to introgress these genes into PRR78 using marker‐assisted backcrossing (MABC). Microsatellite markers RM5926 and AP5659‐5 tightly linked to Pi‐1 and Piz‐5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR78. Most of the improved lines were on par with PRR78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage (RPG) study also revealed similarity of these lines with PRR78. Hybrids derived using improved PRR78 lines were superior over Pusa RH10 in terms of yield.
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spelling CGSpace1657152024-12-22T05:44:52Z Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid Gouda, Patil K. Saikumar, Surapaneni Varma, Chejerla M. K. Nagesh, Kancharla Thippeswamy, Sanka Shenoy, Vinay Ramesha, Mugalodim S. Shashidhar, Halagappa E. backcrossing cooking quality damage disease resistance genes genetic markers genomes hybrids introgression magnaporthe grisea microsatellites pathogens plant breeding plant pathogenic fungi selection varieties Rice blast, caused by fungus Magnaporthe grisea, is a serious disease causing considerable economic damage worldwide. Best way to overcome disease is to breed for disease‐resistant cultivars/parental lines of hybrids. Pusa RH10, first aromatic, fine‐grain rice hybrid released and cultivated extensively in India. Hybrid and its parental lines, Pusa 6A and PRR78, are highly susceptible to blast. CO39 pyramid carrying two dominant, broad‐spectrum blast‐resistance genes, viz. Pi‐1 and Piz‐5, used as a donor parent to introgress these genes into PRR78 using marker‐assisted backcrossing (MABC). Microsatellite markers RM5926 and AP5659‐5 tightly linked to Pi‐1 and Piz‐5 genes, respectively, were used for foreground selection to derive introgression lines. Further, these lines were evaluated for agronomic performance, disease reaction and cooking quality traits along with PRR78. Most of the improved lines were on par with PRR78 for all traits evaluated except gelatinization temperature. Recurrent parent genome percentage (RPG) study also revealed similarity of these lines with PRR78. Hybrids derived using improved PRR78 lines were superior over Pusa RH10 in terms of yield. 2013-02 2024-12-19T12:55:23Z 2024-12-19T12:55:23Z Journal Article https://hdl.handle.net/10568/165715 en Wiley Gouda, Patil K.; Saikumar, Surapaneni; Varma, Chejerla M. K.; Nagesh, Kancharla; Thippeswamy, Sanka; Shenoy, Vinay; Ramesha, Mugalodim S. and Shashidhar, Halagappa E. 2013. Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid. Plant Breeding, Volume 132 no. 1 p. 61-69
spellingShingle backcrossing
cooking quality
damage
disease resistance
genes
genetic markers
genomes
hybrids
introgression
magnaporthe grisea
microsatellites
pathogens
plant breeding
plant pathogenic fungi
selection
varieties
Gouda, Patil K.
Saikumar, Surapaneni
Varma, Chejerla M. K.
Nagesh, Kancharla
Thippeswamy, Sanka
Shenoy, Vinay
Ramesha, Mugalodim S.
Shashidhar, Halagappa E.
Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title_full Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title_fullStr Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title_full_unstemmed Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title_short Marker-assisted breeding of Pi-1 and Piz-5 genes imparting resistance to rice blast in PRR78, restorer line of Pusa RH-10 Basmati rice hybrid
title_sort marker assisted breeding of pi 1 and piz 5 genes imparting resistance to rice blast in prr78 restorer line of pusa rh 10 basmati rice hybrid
topic backcrossing
cooking quality
damage
disease resistance
genes
genetic markers
genomes
hybrids
introgression
magnaporthe grisea
microsatellites
pathogens
plant breeding
plant pathogenic fungi
selection
varieties
url https://hdl.handle.net/10568/165715
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