Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes
Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial blight, which is a major rice disease in tropical Asian countries. An attempt has been made to investigate gene expression patterns of three Xoo strains on the minimal medium XOM2, PXO99 (P6) and PXO86 (P2) from the Philippine...
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| Format: | Journal Article |
| Language: | Inglés |
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Public Library of Science
2013
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| Online Access: | https://hdl.handle.net/10568/165646 |
| _version_ | 1855517803666210816 |
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| author | Zhang, Fan Du, Zhenglin Huang, Liyu Vera Cruz, Casiana Zhou, Yongli Li, Zhikang |
| author_browse | Du, Zhenglin Huang, Liyu Li, Zhikang Vera Cruz, Casiana Zhang, Fan Zhou, Yongli |
| author_facet | Zhang, Fan Du, Zhenglin Huang, Liyu Vera Cruz, Casiana Zhou, Yongli Li, Zhikang |
| author_sort | Zhang, Fan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial blight, which is a major rice disease in tropical Asian countries. An attempt has been made to investigate gene expression patterns of three Xoo strains on the minimal medium XOM2, PXO99 (P6) and PXO86 (P2) from the Philippines, and GD1358 (C5) from China, which exhibited different virulence in 30 rice varieties, with putative virulence factors using deep sequencing. In total, 4,781 transcripts were identified in this study, and 1,151 and 3,076 genes were differentially expressed when P6 was compared with P2 and with C5, respectively. Our results indicated that Xoo strains from different regions exhibited distinctly different expression patterns of putative virulence-relevant genes. Interestingly, 40 and 44 genes involved in chemotaxis and motility exhibited higher transcript alterations in C5 compared with P6 and P2, respectively. Most other genes associated with virulence, including exopolysaccharide (EPS) synthesis, Hrp genes and type III effectors, including Xanthomonas outer protein (Xop) effectors and transcription activator-like (TAL) effectors, were down-regulated in C5 compared with P6 and P2. The data were confirmed by real-time quantitative RT-PCR, tests of bacterial motility, and enzyme activity analysis of EPS and xylanase. These results highlight the complexity of Xoo and offer new avenues for improving our understanding of Xoo-rice interactions and the evolution of Xoo virulence |
| format | Journal Article |
| id | CGSpace165646 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1656462025-05-14T10:23:55Z Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes Zhang, Fan Du, Zhenglin Huang, Liyu Vera Cruz, Casiana Zhou, Yongli Li, Zhikang gene expression genes pathogenicity pathogens plant diseases plant pathogenic bacteria transcription transcriptomes transcriptomics varieties xanthomonas oryzae pv oryzae Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial blight, which is a major rice disease in tropical Asian countries. An attempt has been made to investigate gene expression patterns of three Xoo strains on the minimal medium XOM2, PXO99 (P6) and PXO86 (P2) from the Philippines, and GD1358 (C5) from China, which exhibited different virulence in 30 rice varieties, with putative virulence factors using deep sequencing. In total, 4,781 transcripts were identified in this study, and 1,151 and 3,076 genes were differentially expressed when P6 was compared with P2 and with C5, respectively. Our results indicated that Xoo strains from different regions exhibited distinctly different expression patterns of putative virulence-relevant genes. Interestingly, 40 and 44 genes involved in chemotaxis and motility exhibited higher transcript alterations in C5 compared with P6 and P2, respectively. Most other genes associated with virulence, including exopolysaccharide (EPS) synthesis, Hrp genes and type III effectors, including Xanthomonas outer protein (Xop) effectors and transcription activator-like (TAL) effectors, were down-regulated in C5 compared with P6 and P2. The data were confirmed by real-time quantitative RT-PCR, tests of bacterial motility, and enzyme activity analysis of EPS and xylanase. These results highlight the complexity of Xoo and offer new avenues for improving our understanding of Xoo-rice interactions and the evolution of Xoo virulence 2013-05-29 2024-12-19T12:55:18Z 2024-12-19T12:55:18Z Journal Article https://hdl.handle.net/10568/165646 en Open Access Public Library of Science Zhang, Fan; Du, Zhenglin; Huang, Liyu; Cruz, Casiana Vera; Zhou, Yongli and Li, Zhikang. 2013. Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes. PLoS ONE, Volume 8 no. 5 p. e64267 |
| spellingShingle | gene expression genes pathogenicity pathogens plant diseases plant pathogenic bacteria transcription transcriptomes transcriptomics varieties xanthomonas oryzae pv oryzae Zhang, Fan Du, Zhenglin Huang, Liyu Vera Cruz, Casiana Zhou, Yongli Li, Zhikang Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title | Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title_full | Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title_fullStr | Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title_full_unstemmed | Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title_short | Comparative transcriptome profiling reveals different expression patterns in Xanthomonas oryzae pv. oryzae strains with putative virulence-relevant genes |
| title_sort | comparative transcriptome profiling reveals different expression patterns in xanthomonas oryzae pv oryzae strains with putative virulence relevant genes |
| topic | gene expression genes pathogenicity pathogens plant diseases plant pathogenic bacteria transcription transcriptomes transcriptomics varieties xanthomonas oryzae pv oryzae |
| url | https://hdl.handle.net/10568/165646 |
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