Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley
Inflorescence architecture of barley ( Hordeum vulgare L.) is common among the Triticeae species, which bear one to three single-flowered spikelets at each rachis internode. Triple spikelet meristem is one of the unique features of barley spikes, in which three spikelets (one central and two lateral...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
National Academy of Sciences
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/165623 |
| _version_ | 1855539971544317952 |
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| author | Koppolu, Ravi Anwar, Nadia Sakuma, Shun Tagiri, Akemi Lundqvist, Udda Pourkheirandish, Mohammad Rutten, Twan Seiler, Christiane Himmelbach, Axel Ariyadasa, Ruvini Youssef, Helmy Mohamad Stein, Nils Sreenivasulu, Nese Komatsuda, Takao Schnurbusch, Thorsten |
| author_browse | Anwar, Nadia Ariyadasa, Ruvini Himmelbach, Axel Komatsuda, Takao Koppolu, Ravi Lundqvist, Udda Pourkheirandish, Mohammad Rutten, Twan Sakuma, Shun Schnurbusch, Thorsten Seiler, Christiane Sreenivasulu, Nese Stein, Nils Tagiri, Akemi Youssef, Helmy Mohamad |
| author_facet | Koppolu, Ravi Anwar, Nadia Sakuma, Shun Tagiri, Akemi Lundqvist, Udda Pourkheirandish, Mohammad Rutten, Twan Seiler, Christiane Himmelbach, Axel Ariyadasa, Ruvini Youssef, Helmy Mohamad Stein, Nils Sreenivasulu, Nese Komatsuda, Takao Schnurbusch, Thorsten |
| author_sort | Koppolu, Ravi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Inflorescence architecture of barley ( Hordeum vulgare L.) is common among the Triticeae species, which bear one to three single-flowered spikelets at each rachis internode. Triple spikelet meristem is one of the unique features of barley spikes, in which three spikelets (one central and two lateral spikelets) are produced at each rachis internode. Fertility of the lateral spikelets at triple spikelet meristem gives row-type identity to barley spikes. Six-rowed spikes show fertile lateral spikelets and produce increased grain yield per spike, compared with two-rowed spikes with sterile lateral spikelets. Thus, far, two loci governing the row-type phenotype were isolated in barley that include Six-rowed spike1 ( Vrs1 ) and Intermedium-C . In the present study, we isolated Six-rowed spike4 ( Vrs4 ), a barley ortholog of the maize ( Zea mays L.) inflorescence architecture gene RAMOSA2 ( RA2 ). Eighteen coding mutations in barley RA2 ( HvRA2 ) were specifically associated with lateral spikelet fertility and loss of spikelet determinacy. Expression analyses through mRNA in situ hybridization and microarray showed that Vrs4 ( HvRA2 ) controls the row-type pathway through Vrs1 ( HvHox1 ), a negative regulator of lateral spikelet fertility in barley. Moreover, Vrs4 may also regulate transcripts of barley SISTER OF RAMOSA3 ( HvSRA ), a putative trehalose-6-phosphate phosphatase involved in trehalose-6-phosphate homeostasis implicated to control spikelet determinacy. Our expression data illustrated that, although RA2 is conserved among different grass species, its down-stream target genes appear to be modified in barley and possibly other species of tribe Triticeae. |
| format | Journal Article |
| id | CGSpace165623 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | CGSpace1656232025-12-08T09:54:28Z Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley Koppolu, Ravi Anwar, Nadia Sakuma, Shun Tagiri, Akemi Lundqvist, Udda Pourkheirandish, Mohammad Rutten, Twan Seiler, Christiane Himmelbach, Axel Ariyadasa, Ruvini Youssef, Helmy Mohamad Stein, Nils Sreenivasulu, Nese Komatsuda, Takao Schnurbusch, Thorsten barley enzymes genes in situ hybridization inflorescences internodes loci maize messenger rna mutants mutations phenotypes spikelets Inflorescence architecture of barley ( Hordeum vulgare L.) is common among the Triticeae species, which bear one to three single-flowered spikelets at each rachis internode. Triple spikelet meristem is one of the unique features of barley spikes, in which three spikelets (one central and two lateral spikelets) are produced at each rachis internode. Fertility of the lateral spikelets at triple spikelet meristem gives row-type identity to barley spikes. Six-rowed spikes show fertile lateral spikelets and produce increased grain yield per spike, compared with two-rowed spikes with sterile lateral spikelets. Thus, far, two loci governing the row-type phenotype were isolated in barley that include Six-rowed spike1 ( Vrs1 ) and Intermedium-C . In the present study, we isolated Six-rowed spike4 ( Vrs4 ), a barley ortholog of the maize ( Zea mays L.) inflorescence architecture gene RAMOSA2 ( RA2 ). Eighteen coding mutations in barley RA2 ( HvRA2 ) were specifically associated with lateral spikelet fertility and loss of spikelet determinacy. Expression analyses through mRNA in situ hybridization and microarray showed that Vrs4 ( HvRA2 ) controls the row-type pathway through Vrs1 ( HvHox1 ), a negative regulator of lateral spikelet fertility in barley. Moreover, Vrs4 may also regulate transcripts of barley SISTER OF RAMOSA3 ( HvSRA ), a putative trehalose-6-phosphate phosphatase involved in trehalose-6-phosphate homeostasis implicated to control spikelet determinacy. Our expression data illustrated that, although RA2 is conserved among different grass species, its down-stream target genes appear to be modified in barley and possibly other species of tribe Triticeae. 2013-08-06 2024-12-19T12:55:16Z 2024-12-19T12:55:16Z Journal Article https://hdl.handle.net/10568/165623 en National Academy of Sciences Koppolu, Ravi; Anwar, Nadia; Sakuma, Shun; Tagiri, Akemi; Lundqvist, Udda; Pourkheirandish, Mohammad; Rutten, Twan; Seiler, Christiane; Himmelbach, Axel; Ariyadasa, Ruvini; Youssef, Helmy Mohamad; Stein, Nils; Sreenivasulu, Nese; Komatsuda, Takao and Schnurbusch, Thorsten. 2013. Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley. Proc. Natl. Acad. Sci. U.S.A., Volume 110 no. 32 p. 13198-13203 |
| spellingShingle | barley enzymes genes in situ hybridization inflorescences internodes loci maize messenger rna mutants mutations phenotypes spikelets Koppolu, Ravi Anwar, Nadia Sakuma, Shun Tagiri, Akemi Lundqvist, Udda Pourkheirandish, Mohammad Rutten, Twan Seiler, Christiane Himmelbach, Axel Ariyadasa, Ruvini Youssef, Helmy Mohamad Stein, Nils Sreenivasulu, Nese Komatsuda, Takao Schnurbusch, Thorsten Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title | Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title_full | Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title_fullStr | Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title_full_unstemmed | Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title_short | Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley |
| title_sort | six rowed spike4 vrs4 controls spikelet determinacy and row type in barley |
| topic | barley enzymes genes in situ hybridization inflorescences internodes loci maize messenger rna mutants mutations phenotypes spikelets |
| url | https://hdl.handle.net/10568/165623 |
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