Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway

Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact ofγ-rays on key aspects of plant metabolism still needs to be carefully eval...

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Main Authors: Macovei, Anca, Garg, Bharti, Raikwar, Shailendra, Balestrazzi, Alma, Carbonera, Daniela, Buttafava, Armando, Bremont, Juan Francisco Jiménez, Gill, Sarvajeet Singh, Tuteja, Narendra
Format: Journal Article
Language:Inglés
Published: Hindawi Limited 2014
Subjects:
Online Access:https://hdl.handle.net/10568/165563
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author Macovei, Anca
Garg, Bharti
Raikwar, Shailendra
Balestrazzi, Alma
Carbonera, Daniela
Buttafava, Armando
Bremont, Juan Francisco Jiménez
Gill, Sarvajeet Singh
Tuteja, Narendra
author_browse Balestrazzi, Alma
Bremont, Juan Francisco Jiménez
Buttafava, Armando
Carbonera, Daniela
Garg, Bharti
Gill, Sarvajeet Singh
Macovei, Anca
Raikwar, Shailendra
Tuteja, Narendra
author_facet Macovei, Anca
Garg, Bharti
Raikwar, Shailendra
Balestrazzi, Alma
Carbonera, Daniela
Buttafava, Armando
Bremont, Juan Francisco Jiménez
Gill, Sarvajeet Singh
Tuteja, Narendra
author_sort Macovei, Anca
collection Repository of Agricultural Research Outputs (CGSpace)
description Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact ofγ-rays on key aspects of plant metabolism still needs to be carefully evaluated. In the present study, rice seeds were challenged with different doses ofγ-rays and grown in absence/presence of NaCl to assess the impact of these treatments on the early stages of plant life. Enhanced germination efficiency associated with increase in radicle and hypocotyl length was observed, while at later stages no increase in plant tolerance to salinity stress was evident. APX, CAT, and GR were enhanced at transcriptional level and in terms of enzyme activity, indicating the activation of antioxidant defence. The profiles of DNA damage accumulation were obtained using SCGE and the implication of TC-NER pathway in DNA damage sensing and repair mechanisms is discussed.OsXPB2,OsXPD,OsTFIIS,andOsTFIIS-likegenes showed differential modulation in seedlings and plantlets in response toγ-irradiation and salinity stress. Altogether, the synergistic exposure toγ-rays and NaCl resulted in enhanced oxidative stress and proper activation of antioxidant mechanisms, thus being compatible with plant survival.
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publishDate 2014
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spelling CGSpace1655632025-12-08T10:29:22Z Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway Macovei, Anca Garg, Bharti Raikwar, Shailendra Balestrazzi, Alma Carbonera, Daniela Buttafava, Armando Bremont, Juan Francisco Jiménez Gill, Sarvajeet Singh Tuteja, Narendra abiotic stress antioxidants biotic stress gamma radiation pregermination salinity salt stress seeds Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact ofγ-rays on key aspects of plant metabolism still needs to be carefully evaluated. In the present study, rice seeds were challenged with different doses ofγ-rays and grown in absence/presence of NaCl to assess the impact of these treatments on the early stages of plant life. Enhanced germination efficiency associated with increase in radicle and hypocotyl length was observed, while at later stages no increase in plant tolerance to salinity stress was evident. APX, CAT, and GR were enhanced at transcriptional level and in terms of enzyme activity, indicating the activation of antioxidant defence. The profiles of DNA damage accumulation were obtained using SCGE and the implication of TC-NER pathway in DNA damage sensing and repair mechanisms is discussed.OsXPB2,OsXPD,OsTFIIS,andOsTFIIS-likegenes showed differential modulation in seedlings and plantlets in response toγ-irradiation and salinity stress. Altogether, the synergistic exposure toγ-rays and NaCl resulted in enhanced oxidative stress and proper activation of antioxidant mechanisms, thus being compatible with plant survival. 2014 2024-12-19T12:55:11Z 2024-12-19T12:55:11Z Journal Article https://hdl.handle.net/10568/165563 en Open Access Hindawi Limited Macovei, Anca; Garg, Bharti; Raikwar, Shailendra; Balestrazzi, Alma; Carbonera, Daniela; Buttafava, Armando; Bremont, Juan Francisco Jiménez; Gill, Sarvajeet Singh and Tuteja, Narendra. 2014. Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway. BioMed Research International, Volume 2014 p. 1-15
spellingShingle abiotic stress
antioxidants
biotic stress
gamma radiation
pregermination
salinity
salt stress
seeds
Macovei, Anca
Garg, Bharti
Raikwar, Shailendra
Balestrazzi, Alma
Carbonera, Daniela
Buttafava, Armando
Bremont, Juan Francisco Jiménez
Gill, Sarvajeet Singh
Tuteja, Narendra
Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title_full Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title_fullStr Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title_full_unstemmed Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title_short Synergistic exposure of rice seeds to different doses of gamma-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway
title_sort synergistic exposure of rice seeds to different doses of gamma ray and salinity stress resulted in increased antioxidant enzyme activities and gene specific modulation of tc ner pathway
topic abiotic stress
antioxidants
biotic stress
gamma radiation
pregermination
salinity
salt stress
seeds
url https://hdl.handle.net/10568/165563
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