Identification and characterization of suppressor mutants of spl11-mediated cell death in rice
Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
2014
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| Online Access: | https://hdl.handle.net/10568/165515 |
| _version_ | 1855533306657898496 |
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| author | Shirsekar, Gautam S. Vega-Sanchez, Miguel E. Bordeos, Alicia Baraoidan, Marietta Swisshelm, Andrew Fan, Jiangbo Park, Chan Ho Leung, Hei Wang, Guo-Liang |
| author_browse | Baraoidan, Marietta Bordeos, Alicia Fan, Jiangbo Leung, Hei Park, Chan Ho Shirsekar, Gautam S. Swisshelm, Andrew Vega-Sanchez, Miguel E. Wang, Guo-Liang |
| author_facet | Shirsekar, Gautam S. Vega-Sanchez, Miguel E. Bordeos, Alicia Baraoidan, Marietta Swisshelm, Andrew Fan, Jiangbo Park, Chan Ho Leung, Hei Wang, Guo-Liang |
| author_sort | Shirsekar, Gautam S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway. |
| format | Journal Article |
| id | CGSpace165515 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1655152024-12-19T14:13:09Z Identification and characterization of suppressor mutants of spl11-mediated cell death in rice Shirsekar, Gautam S. Vega-Sanchez, Miguel E. Bordeos, Alicia Baraoidan, Marietta Swisshelm, Andrew Fan, Jiangbo Park, Chan Ho Leung, Hei Wang, Guo-Liang chromosomes disease resistance enzymes fungi genes magnaporthe grisea mutants mutations pathogens phenotypes plant pathogenic bacteria plant pathogenic fungi xanthomonas oryzae pv oryzae Lesion mimic mutants have been used to dissect programmed cell death (PCD) and defense-related pathways in plants. The rice lesion-mimic mutant spl11 exhibits race nonspecific resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae and the fungal pathogen Magnaporthe oryzae. Spl11 encodes an E3 ubiquitin ligase and is a negative regulator of PCD in rice. To study the regulation of Spl11-mediated PCD, we performed a genetic screen and identified three spl11 cell-death suppressor (sds) mutants. These suppressors were characterized for their resistance to X. oryzae pv. oryzae and M. oryzae and for their expression of defense-related genes. The suppression of the cell-death phenotypes was generally correlated with reduced expression of defense-related genes. When rice was challenged with avirulent isolates of M. oryzae, the disease phenotype was unaffected in the sds mutants, indicating that the suppression might be Spl11-mediated pathway specific and may only be involved in basal defense. In addition, we mapped one of the suppressor mutations to a 140-kb interval on the long arm of rice chromosome 1. Identification and characterization of these sds mutants should facilitate our efforts to elucidate the Spl11-mediated PCD pathway. 2014-06 2024-12-19T12:55:09Z 2024-12-19T12:55:09Z Journal Article https://hdl.handle.net/10568/165515 en Scientific Societies Shirsekar, Gautam S.; Vega-Sanchez, Miguel E.; Bordeos, Alicia; Baraoidan, Marietta; Swisshelm, Andrew; Fan, Jiangbo; Park, Chan Ho; Leung, Hei and Wang, Guo-Liang. 2014. Identification and characterization of suppressor mutants of spl11-mediated cell death in rice. MPMI, Volume 27 no. 6 p. 528-536 |
| spellingShingle | chromosomes disease resistance enzymes fungi genes magnaporthe grisea mutants mutations pathogens phenotypes plant pathogenic bacteria plant pathogenic fungi xanthomonas oryzae pv oryzae Shirsekar, Gautam S. Vega-Sanchez, Miguel E. Bordeos, Alicia Baraoidan, Marietta Swisshelm, Andrew Fan, Jiangbo Park, Chan Ho Leung, Hei Wang, Guo-Liang Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title | Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title_full | Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title_fullStr | Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title_full_unstemmed | Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title_short | Identification and characterization of suppressor mutants of spl11-mediated cell death in rice |
| title_sort | identification and characterization of suppressor mutants of spl11 mediated cell death in rice |
| topic | chromosomes disease resistance enzymes fungi genes magnaporthe grisea mutants mutations pathogens phenotypes plant pathogenic bacteria plant pathogenic fungi xanthomonas oryzae pv oryzae |
| url | https://hdl.handle.net/10568/165515 |
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