Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice

Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. In this study, a rice introgression line (IL), FF329, which was identified from a BC1F4 population derived from the cross between donor PSBRC66 (P66) and recipient Huang‐Hua‐...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, F., Zhuo, D.‐L., Huang, L.‐Y., Wang, W.‐S., Xu, J.‐L., Vera Cruz, C., Li, Z.‐K., Zhou, Y.‐L.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2015
Acceso en línea:https://hdl.handle.net/10568/165486
_version_ 1855526306397028352
author Zhang, F.
Zhuo, D.‐L.
Zhang, F.
Huang, L.‐Y.
Wang, W.‐S.
Xu, J.‐L.
Vera Cruz, C.
Li, Z.‐K.
Zhou, Y.‐L.
author_browse Huang, L.‐Y.
Li, Z.‐K.
Vera Cruz, C.
Wang, W.‐S.
Xu, J.‐L.
Zhang, F.
Zhou, Y.‐L.
Zhuo, D.‐L.
author_facet Zhang, F.
Zhuo, D.‐L.
Zhang, F.
Huang, L.‐Y.
Wang, W.‐S.
Xu, J.‐L.
Vera Cruz, C.
Li, Z.‐K.
Zhou, Y.‐L.
author_sort Zhang, F.
collection Repository of Agricultural Research Outputs (CGSpace)
description Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. In this study, a rice introgression line (IL), FF329, which was identified from a BC1F4 population derived from the cross between donor PSBRC66 (P66) and recipient Huang‐Hua‐Zhan (HHZ), exhibited a typical hypersensitive response (HR) when inoculated with all 21 representative Xoo strains, consisting of 14 Philippines races and seven Chinese pathotypes. By contrast, the parents were highly susceptible to 10 of the tested strains and resistant or moderately susceptible to 11 strains, but without HR symptoms. Genetic analysis of the F2 population derived from the HHZ/FF329 cross with virulent Philippines race 6 (P6) and Chinese pathotype V (CV) revealed that BB resistance in FF329 was controlled by a single dominant gene. Because FF329 showed broad‐spectrum BB resistance, different from both parents, the gene identified was novel and was designated Xa39. Two SSR markers, RM21 and RM206, located on rice chromosome 11, were linked to the target gene by bulked segregant analysis. A further six markers linked to Xa39 were identified in the region between RM21 and RM206 for fine gene mapping, and the Xa39 locus was refined to a 97·4 kb interval flanked by markers RM26985 and DM13 using a large F2 population. This gene provides rice breeders with a new option to incorporate BB resistance into newly developed cultivars.
format Journal Article
id CGSpace165486
institution CGIAR Consortium
language Inglés
publishDate 2015
publishDateRange 2015
publishDateSort 2015
publisher Wiley
publisherStr Wiley
record_format dspace
spelling CGSpace1654862026-01-06T12:03:48Z Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice Zhang, F. Zhuo, D.‐L. Zhang, F. Huang, L.‐Y. Wang, W.‐S. Xu, J.‐L. Vera Cruz, C. Li, Z.‐K. Zhou, Y.‐L. Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. In this study, a rice introgression line (IL), FF329, which was identified from a BC1F4 population derived from the cross between donor PSBRC66 (P66) and recipient Huang‐Hua‐Zhan (HHZ), exhibited a typical hypersensitive response (HR) when inoculated with all 21 representative Xoo strains, consisting of 14 Philippines races and seven Chinese pathotypes. By contrast, the parents were highly susceptible to 10 of the tested strains and resistant or moderately susceptible to 11 strains, but without HR symptoms. Genetic analysis of the F2 population derived from the HHZ/FF329 cross with virulent Philippines race 6 (P6) and Chinese pathotype V (CV) revealed that BB resistance in FF329 was controlled by a single dominant gene. Because FF329 showed broad‐spectrum BB resistance, different from both parents, the gene identified was novel and was designated Xa39. Two SSR markers, RM21 and RM206, located on rice chromosome 11, were linked to the target gene by bulked segregant analysis. A further six markers linked to Xa39 were identified in the region between RM21 and RM206 for fine gene mapping, and the Xa39 locus was refined to a 97·4 kb interval flanked by markers RM26985 and DM13 using a large F2 population. This gene provides rice breeders with a new option to incorporate BB resistance into newly developed cultivars. 2015-06 2024-12-19T12:55:08Z 2024-12-19T12:55:08Z Journal Article https://hdl.handle.net/10568/165486 en Wiley Zhang, F.; Zhuo, D.‐L.; Zhang, F.; Huang, L.‐Y.; Wang, W.‐S.; Xu, J.‐L.; Vera Cruz, C.; Li, Z.‐K. and Zhou, Y.‐L. 2015. Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice. Plant Pathology, Volume 64 no. 3 p. 568-575
spellingShingle Zhang, F.
Zhuo, D.‐L.
Zhang, F.
Huang, L.‐Y.
Wang, W.‐S.
Xu, J.‐L.
Vera Cruz, C.
Li, Z.‐K.
Zhou, Y.‐L.
Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title_full Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title_fullStr Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title_full_unstemmed Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title_short Xa39, a novel dominant gene conferring broad‐spectrum resistance to Xanthomonas oryzae pv. oryzae in rice
title_sort xa39 a novel dominant gene conferring broad spectrum resistance to xanthomonas oryzae pv oryzae in rice
url https://hdl.handle.net/10568/165486
work_keys_str_mv AT zhangf xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT zhuodl xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT zhangf xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT huangly xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT wangws xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT xujl xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT veracruzc xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT lizk xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice
AT zhouyl xa39anoveldominantgeneconferringbroadspectrumresistancetoxanthomonasoryzaepvoryzaeinrice