A genetic strategy generating wheat with very high amylose content
Resistant starch (RS), a type of dietary fibre, plays an important role in human health; however, the content of RS in most modern processed starchy foods is low. Cereal starch, when structurally manipulated through a modified starch biosynthetic pathway to greatly increase the amylose content, coul...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2015
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| Acceso en línea: | https://hdl.handle.net/10568/165426 |
| _version_ | 1855519661758611456 |
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| author | Regina, Ahmed Berbezy, Pierre Kosar-Hashemi, Behjat Li, Suzhi Cmiel, Mark Larroque, Oscar Bird, Anthony R. Swain, Steve M. Cavanagh, Colin Jobling, Stephen A. Li, Zhongyi Morell, Matthew |
| author_browse | Berbezy, Pierre Bird, Anthony R. Cavanagh, Colin Cmiel, Mark Jobling, Stephen A. Kosar-Hashemi, Behjat Larroque, Oscar Li, Suzhi Li, Zhongyi Morell, Matthew Regina, Ahmed Swain, Steve M. |
| author_facet | Regina, Ahmed Berbezy, Pierre Kosar-Hashemi, Behjat Li, Suzhi Cmiel, Mark Larroque, Oscar Bird, Anthony R. Swain, Steve M. Cavanagh, Colin Jobling, Stephen A. Li, Zhongyi Morell, Matthew |
| author_sort | Regina, Ahmed |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Resistant starch (RS), a type of dietary fibre, plays an important role in human health; however, the content of RS in most modern processed starchy foods is low. Cereal starch, when structurally manipulated through a modified starch biosynthetic pathway to greatly increase the amylose content, could be an important food source of RS. Transgenic studies have previously revealed the requirement of simultaneous down‐regulation of two starch branching enzyme (SBE) II isoforms both located on the long arm of chromosome 2, namely SBEIIa and SBEIIb, to elevate the amylose content in wheat from ~25% to ~75%. The current study revealed close proximity of genes encoding SBEIIa and SBEIIb isoforms in wheat with a genetic distance of 0.5 cM on chromosome 2B. A series of deletion and single nucleotide polymorphism (SNP) loss of function alleles in SBEIIa, SBEIIb or both was isolated from two different wheat populations. A breeding strategy to combine deletions and SNPs generated wheat genotypes with altered expression levels of SBEIIa and SBEIIb, elevating the amylose content to an unprecedented ~85%, with a marked concomitant increase in RS content. Biochemical assays were used to confirm the complete absence in the grain of expression of SBEIIa from all three genomes in combination with the absence of SBEIIb from one of the genomes. |
| format | Journal Article |
| id | CGSpace165426 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1654262025-12-08T09:54:28Z A genetic strategy generating wheat with very high amylose content Regina, Ahmed Berbezy, Pierre Kosar-Hashemi, Behjat Li, Suzhi Cmiel, Mark Larroque, Oscar Bird, Anthony R. Swain, Steve M. Cavanagh, Colin Jobling, Stephen A. Li, Zhongyi Morell, Matthew Resistant starch (RS), a type of dietary fibre, plays an important role in human health; however, the content of RS in most modern processed starchy foods is low. Cereal starch, when structurally manipulated through a modified starch biosynthetic pathway to greatly increase the amylose content, could be an important food source of RS. Transgenic studies have previously revealed the requirement of simultaneous down‐regulation of two starch branching enzyme (SBE) II isoforms both located on the long arm of chromosome 2, namely SBEIIa and SBEIIb, to elevate the amylose content in wheat from ~25% to ~75%. The current study revealed close proximity of genes encoding SBEIIa and SBEIIb isoforms in wheat with a genetic distance of 0.5 cM on chromosome 2B. A series of deletion and single nucleotide polymorphism (SNP) loss of function alleles in SBEIIa, SBEIIb or both was isolated from two different wheat populations. A breeding strategy to combine deletions and SNPs generated wheat genotypes with altered expression levels of SBEIIa and SBEIIb, elevating the amylose content to an unprecedented ~85%, with a marked concomitant increase in RS content. Biochemical assays were used to confirm the complete absence in the grain of expression of SBEIIa from all three genomes in combination with the absence of SBEIIb from one of the genomes. 2015-12 2024-12-19T12:55:03Z 2024-12-19T12:55:03Z Journal Article https://hdl.handle.net/10568/165426 en Wiley Regina, Ahmed; Berbezy, Pierre; Kosar‐Hashemi, Behjat; Li, Suzhi; Cmiel, Mark; Larroque, Oscar; Bird, Anthony R.; Swain, Steve M.; Cavanagh, Colin; Jobling, Stephen A.; Li, Zhongyi and Morell, Matthew. 2015. A genetic strategy generating wheat with very high amylose content. Plant Biotechnology Journal, Volume 13 no. 9 p. 1276-1286 |
| spellingShingle | Regina, Ahmed Berbezy, Pierre Kosar-Hashemi, Behjat Li, Suzhi Cmiel, Mark Larroque, Oscar Bird, Anthony R. Swain, Steve M. Cavanagh, Colin Jobling, Stephen A. Li, Zhongyi Morell, Matthew A genetic strategy generating wheat with very high amylose content |
| title | A genetic strategy generating wheat with very high amylose content |
| title_full | A genetic strategy generating wheat with very high amylose content |
| title_fullStr | A genetic strategy generating wheat with very high amylose content |
| title_full_unstemmed | A genetic strategy generating wheat with very high amylose content |
| title_short | A genetic strategy generating wheat with very high amylose content |
| title_sort | genetic strategy generating wheat with very high amylose content |
| url | https://hdl.handle.net/10568/165426 |
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