OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice
Although 14-3-3 proteins have been reported to be involved in responses to biotic stresses in plants, their functions in rice blast, the most destructive disease in rice, are largely unknown. Only GF14e has been confirmed to negatively regulate leaf blast. We report that GF14b is highly expressed in...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/165321 |
| _version_ | 1855531230064279552 |
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| author | Liu, Qing Yang, Jianyuan Zhang, Shaohong Zhao, Junliang Feng, Aiqing Yang, Tifeng Wang, Xiaofei Mao, Xinxue Dong, Jingfang Zhu, Xiaoyuan Leung, Hei Leach, Jan E. Liu, Bin |
| author_browse | Dong, Jingfang Feng, Aiqing Leach, Jan E. Leung, Hei Liu, Bin Liu, Qing Mao, Xinxue Wang, Xiaofei Yang, Jianyuan Yang, Tifeng Zhang, Shaohong Zhao, Junliang Zhu, Xiaoyuan |
| author_facet | Liu, Qing Yang, Jianyuan Zhang, Shaohong Zhao, Junliang Feng, Aiqing Yang, Tifeng Wang, Xiaofei Mao, Xinxue Dong, Jingfang Zhu, Xiaoyuan Leung, Hei Leach, Jan E. Liu, Bin |
| author_sort | Liu, Qing |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Although 14-3-3 proteins have been reported to be involved in responses to biotic stresses in plants, their functions in rice blast, the most destructive disease in rice, are largely unknown. Only GF14e has been confirmed to negatively regulate leaf blast. We report that GF14b is highly expressed in seedlings and panicles during blast infection. Rice plants overexpressing GF14b show enhanced resistance to panicle blast but are susceptible to leaf blast. In contrast, GF14b-silenced plants show increased susceptibility to panicle blast but enhanced resistance to leaf blast. Yeast one-hybrid assays demonstrate that WRKY71 binds to the promoter of GF14b and modulates its expression. Overexpression of GF14b induces expression of jasmonic acid (JA) synthesis-related genes but suppresses expression of salicylic acid (SA) synthesis-related genes. In contrast, suppressed GF14b expression causes decreased expression of JA synthesis-related genes but activation of SA synthesis-related genes. These results suggest that GF14b positively regulates panicle blast resistance but negatively regulates leaf blast resistance, and that GF14b-mediated disease resistance is associated with the JA- and SA-dependent pathway. The different functions for 14-3-3 proteins in leaf and panicle blast provide new evidence that leaf and panicle blast resistance are controlled by different mechanisms. |
| format | Journal Article |
| id | CGSpace165321 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1653212025-12-08T09:54:28Z OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice Liu, Qing Yang, Jianyuan Zhang, Shaohong Zhao, Junliang Feng, Aiqing Yang, Tifeng Wang, Xiaofei Mao, Xinxue Dong, Jingfang Zhu, Xiaoyuan Leung, Hei Leach, Jan E. Liu, Bin biotic stress blast disease resistance gene expression inflorescences overexpression Although 14-3-3 proteins have been reported to be involved in responses to biotic stresses in plants, their functions in rice blast, the most destructive disease in rice, are largely unknown. Only GF14e has been confirmed to negatively regulate leaf blast. We report that GF14b is highly expressed in seedlings and panicles during blast infection. Rice plants overexpressing GF14b show enhanced resistance to panicle blast but are susceptible to leaf blast. In contrast, GF14b-silenced plants show increased susceptibility to panicle blast but enhanced resistance to leaf blast. Yeast one-hybrid assays demonstrate that WRKY71 binds to the promoter of GF14b and modulates its expression. Overexpression of GF14b induces expression of jasmonic acid (JA) synthesis-related genes but suppresses expression of salicylic acid (SA) synthesis-related genes. In contrast, suppressed GF14b expression causes decreased expression of JA synthesis-related genes but activation of SA synthesis-related genes. These results suggest that GF14b positively regulates panicle blast resistance but negatively regulates leaf blast resistance, and that GF14b-mediated disease resistance is associated with the JA- and SA-dependent pathway. The different functions for 14-3-3 proteins in leaf and panicle blast provide new evidence that leaf and panicle blast resistance are controlled by different mechanisms. 2016-01 2024-12-19T12:54:56Z 2024-12-19T12:54:56Z Journal Article https://hdl.handle.net/10568/165321 en Scientific Societies Liu, Qing; Yang, Jianyuan; Zhang, Shaohong; Zhao, Junliang; Feng, Aiqing; Yang, Tifeng; Wang, Xiaofei; Mao, Xinxue; Dong, Jingfang; Zhu, Xiaoyuan; Leung, Hei; Leach, Jan E. and Liu, Bin. 2016. OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice. MPMI, Volume 29 no. 1 p. 46-56 |
| spellingShingle | biotic stress blast disease resistance gene expression inflorescences overexpression Liu, Qing Yang, Jianyuan Zhang, Shaohong Zhao, Junliang Feng, Aiqing Yang, Tifeng Wang, Xiaofei Mao, Xinxue Dong, Jingfang Zhu, Xiaoyuan Leung, Hei Leach, Jan E. Liu, Bin OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title | OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title_full | OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title_fullStr | OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title_full_unstemmed | OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title_short | OsGF14b positively regulates panicle blast resistance, but negatively regulates leaf blast resistance in rice |
| title_sort | osgf14b positively regulates panicle blast resistance but negatively regulates leaf blast resistance in rice |
| topic | biotic stress blast disease resistance gene expression inflorescences overexpression |
| url | https://hdl.handle.net/10568/165321 |
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