Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae
Resistance in rice cultivars to the rice blast fungus Magnaporthe oryzae is complex and is controlled by both major genes and quantitative trait loci (QTLs). We undertook a genome‐wide association study (GWAS) using the rice diversity panel 1 (RDP1) that was genotyped using a high‐density (700 000 s...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2016
|
| Online Access: | https://hdl.handle.net/10568/165295 |
| _version_ | 1855521518668218368 |
|---|---|
| author | Kang, Houxiang Wang, Yue Peng, Shasha Zhang, Yanli Xiao, Yinghui Wang, Dan Qu, Shaohong Li, Zhiqiang Yan, Shuangyong Wang, Zhilong Liu, Wende Ning, Yuese Korniliev, Pavel Leung, Hei Mezey, Jason McCouch, Susan R. Wang, Guo-Liang |
| author_browse | Kang, Houxiang Korniliev, Pavel Leung, Hei Li, Zhiqiang Liu, Wende McCouch, Susan R. Mezey, Jason Ning, Yuese Peng, Shasha Qu, Shaohong Wang, Dan Wang, Guo-Liang Wang, Yue Wang, Zhilong Xiao, Yinghui Yan, Shuangyong Zhang, Yanli |
| author_facet | Kang, Houxiang Wang, Yue Peng, Shasha Zhang, Yanli Xiao, Yinghui Wang, Dan Qu, Shaohong Li, Zhiqiang Yan, Shuangyong Wang, Zhilong Liu, Wende Ning, Yuese Korniliev, Pavel Leung, Hei Mezey, Jason McCouch, Susan R. Wang, Guo-Liang |
| author_sort | Kang, Houxiang |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Resistance in rice cultivars to the rice blast fungus Magnaporthe oryzae is complex and is controlled by both major genes and quantitative trait loci (QTLs). We undertook a genome‐wide association study (GWAS) using the rice diversity panel 1 (RDP1) that was genotyped using a high‐density (700 000 single nucleotide polymorphisms) array and inoculated with five diverse M. oryzae isolates. We identified 97 loci associated with blast resistance (LABRs). Among them, 82 were new regions and 15 co‐localized with known blast resistance loci. The top 72 LABRs explained up to 98% of the phenotypic variation. The candidate genes in the LABRs encode nucleotide‐binding site leucine‐rich repeat (NBS‐LRR) resistance proteins, receptor‐like protein kinases, transcription factors and defence‐related proteins. Among them, LABR_64 was strongly associated with resistance to all five isolates. We analysed the function of candidate genes underlying LABR_64 using RNA interference (RNAi) technology and identified two new resistance alleles at the Pi5 locus. We demonstrate an efficient strategy for rapid allele discovery using the power of GWAS, coupled with RNAi technology, for the dissection of complex blast resistance in rice. |
| format | Journal Article |
| id | CGSpace165295 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1652952025-05-14T10:24:01Z Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae Kang, Houxiang Wang, Yue Peng, Shasha Zhang, Yanli Xiao, Yinghui Wang, Dan Qu, Shaohong Li, Zhiqiang Yan, Shuangyong Wang, Zhilong Liu, Wende Ning, Yuese Korniliev, Pavel Leung, Hei Mezey, Jason McCouch, Susan R. Wang, Guo-Liang Resistance in rice cultivars to the rice blast fungus Magnaporthe oryzae is complex and is controlled by both major genes and quantitative trait loci (QTLs). We undertook a genome‐wide association study (GWAS) using the rice diversity panel 1 (RDP1) that was genotyped using a high‐density (700 000 single nucleotide polymorphisms) array and inoculated with five diverse M. oryzae isolates. We identified 97 loci associated with blast resistance (LABRs). Among them, 82 were new regions and 15 co‐localized with known blast resistance loci. The top 72 LABRs explained up to 98% of the phenotypic variation. The candidate genes in the LABRs encode nucleotide‐binding site leucine‐rich repeat (NBS‐LRR) resistance proteins, receptor‐like protein kinases, transcription factors and defence‐related proteins. Among them, LABR_64 was strongly associated with resistance to all five isolates. We analysed the function of candidate genes underlying LABR_64 using RNA interference (RNAi) technology and identified two new resistance alleles at the Pi5 locus. We demonstrate an efficient strategy for rapid allele discovery using the power of GWAS, coupled with RNAi technology, for the dissection of complex blast resistance in rice. 2016-08 2024-12-19T12:54:55Z 2024-12-19T12:54:55Z Journal Article https://hdl.handle.net/10568/165295 en Wiley Kang, Houxiang; Wang, Yue; Peng, Shasha; Zhang, Yanli; Xiao, Yinghui; Wang, Dan; Qu, Shaohong; Li, Zhiqiang; Yan, Shuangyong; Wang, Zhilong; Liu, Wende; Ning, Yuese; Korniliev, Pavel; Leung, Hei; Mezey, Jason; McCouch, Susan R. and Wang, Guo‐Liang. 2016. Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae. Molecular Plant Pathology, Volume 17 no. 6 p. 959-972 |
| spellingShingle | Kang, Houxiang Wang, Yue Peng, Shasha Zhang, Yanli Xiao, Yinghui Wang, Dan Qu, Shaohong Li, Zhiqiang Yan, Shuangyong Wang, Zhilong Liu, Wende Ning, Yuese Korniliev, Pavel Leung, Hei Mezey, Jason McCouch, Susan R. Wang, Guo-Liang Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title | Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title_full | Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title_fullStr | Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title_full_unstemmed | Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title_short | Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae |
| title_sort | dissection of the genetic architecture of rice resistance to the blast fungus magnaporthe oryzae |
| url | https://hdl.handle.net/10568/165295 |
| work_keys_str_mv | AT kanghouxiang dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT wangyue dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT pengshasha dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT zhangyanli dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT xiaoyinghui dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT wangdan dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT qushaohong dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT lizhiqiang dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT yanshuangyong dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT wangzhilong dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT liuwende dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT ningyuese dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT kornilievpavel dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT leunghei dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT mezeyjason dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT mccouchsusanr dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae AT wangguoliang dissectionofthegeneticarchitectureofriceresistancetotheblastfungusmagnaportheoryzae |