Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18

Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramkumar, Gandhimani, Prahalada, G.D., Hechanova, Sherry Lou, Kim, Sung-Ryul, Jena, Kshirod K.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2016
Acceso en línea:https://hdl.handle.net/10568/165258
_version_ 1855518615662493696
author Ramkumar, Gandhimani
Prahalada, G.D.
Hechanova, Sherry Lou
Kim, Sung-Ryul
Jena, Kshirod K.
author_browse Hechanova, Sherry Lou
Jena, Kshirod K.
Kim, Sung-Ryul
Prahalada, G.D.
Ramkumar, Gandhimani
author_facet Ramkumar, Gandhimani
Prahalada, G.D.
Hechanova, Sherry Lou
Kim, Sung-Ryul
Jena, Kshirod K.
author_sort Ramkumar, Gandhimani
collection Repository of Agricultural Research Outputs (CGSpace)
description Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene‐specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR‐based co‐dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH‐resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH‐resistant breeding programmes.
format Journal Article
id CGSpace165258
institution CGIAR Consortium
language Inglés
publishDate 2016
publishDateRange 2016
publishDateSort 2016
publisher Wiley
publisherStr Wiley
record_format dspace
spelling CGSpace1652582025-05-14T10:39:33Z Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18 Ramkumar, Gandhimani Prahalada, G.D. Hechanova, Sherry Lou Kim, Sung-Ryul Jena, Kshirod K. Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene‐specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR‐based co‐dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH‐resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH‐resistant breeding programmes. 2016-06 2024-12-19T12:54:51Z 2024-12-19T12:54:51Z Journal Article https://hdl.handle.net/10568/165258 en Wiley Ramkumar, Gandhimani; Prahalada, G. D.; Hechanova, Sherry Lou; Kim, Sung‐Ryul and Jena, Kshirod K. 2016. Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18. Plant Breeding, Volume 135 no. 3 p. 301-308
spellingShingle Ramkumar, Gandhimani
Prahalada, G.D.
Hechanova, Sherry Lou
Kim, Sung-Ryul
Jena, Kshirod K.
Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title_full Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title_fullStr Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title_full_unstemmed Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title_short Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18
title_sort exploring genetic diversity of rice cultivars for the presence of brown planthopper bph resistance genes and development of snp marker for bph18
url https://hdl.handle.net/10568/165258
work_keys_str_mv AT ramkumargandhimani exploringgeneticdiversityofricecultivarsforthepresenceofbrownplanthopperbphresistancegenesanddevelopmentofsnpmarkerforbph18
AT prahaladagd exploringgeneticdiversityofricecultivarsforthepresenceofbrownplanthopperbphresistancegenesanddevelopmentofsnpmarkerforbph18
AT hechanovasherrylou exploringgeneticdiversityofricecultivarsforthepresenceofbrownplanthopperbphresistancegenesanddevelopmentofsnpmarkerforbph18
AT kimsungryul exploringgeneticdiversityofricecultivarsforthepresenceofbrownplanthopperbphresistancegenesanddevelopmentofsnpmarkerforbph18
AT jenakshirodk exploringgeneticdiversityofricecultivarsforthepresenceofbrownplanthopperbphresistancegenesanddevelopmentofsnpmarkerforbph18