DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses

DNA (class 2) transposons are mobile genetic elements which move within their ‘host’ genome through excising and re-inserting elsewhere. Although the rice genome contains tens of thousands of such elements, their actual role in evolution is still unclear. Analysing over 650 transposon polymorphisms...

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Main Authors: Wicker, Thomas, Yu, Yeisoo, Haberer, Georg, Mayer, Klaus F. X., Marri, Pradeep Reddy, Rounsley, Steve, Chen, Mingsheng, Zuccolo, Andrea, Panaud, Olivier, Wing, Rod A., Roffler, Stefan
Format: Journal Article
Language:Inglés
Published: Springer 2016
Online Access:https://hdl.handle.net/10568/165214
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author Wicker, Thomas
Yu, Yeisoo
Haberer, Georg
Mayer, Klaus F. X.
Marri, Pradeep Reddy
Rounsley, Steve
Chen, Mingsheng
Zuccolo, Andrea
Panaud, Olivier
Wing, Rod A.
Roffler, Stefan
author_browse Chen, Mingsheng
Haberer, Georg
Marri, Pradeep Reddy
Mayer, Klaus F. X.
Panaud, Olivier
Roffler, Stefan
Rounsley, Steve
Wicker, Thomas
Wing, Rod A.
Yu, Yeisoo
Zuccolo, Andrea
author_facet Wicker, Thomas
Yu, Yeisoo
Haberer, Georg
Mayer, Klaus F. X.
Marri, Pradeep Reddy
Rounsley, Steve
Chen, Mingsheng
Zuccolo, Andrea
Panaud, Olivier
Wing, Rod A.
Roffler, Stefan
author_sort Wicker, Thomas
collection Repository of Agricultural Research Outputs (CGSpace)
description DNA (class 2) transposons are mobile genetic elements which move within their ‘host’ genome through excising and re-inserting elsewhere. Although the rice genome contains tens of thousands of such elements, their actual role in evolution is still unclear. Analysing over 650 transposon polymorphisms in the rice species Oryza sativa and Oryza glaberrima, we find that DNA repair following transposon excisions is associated with an increased number of mutations in the sequences neighbouring the transposon. Indeed, the 3,000 bp flanking the excised transposons can contain over 10 times more mutations than the genome-wide average. Since DNA transposons preferably insert near genes, this is correlated with increases in mutation rates in coding sequences and regulatory regions. Most importantly, we find this phenomenon also in maize, wheat and barley. Thus, these findings suggest that DNA transposon activity is a major evolutionary force in grasses which provide the basis of most food consumed by humankind.
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spelling CGSpace1652142024-12-19T14:12:43Z DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses Wicker, Thomas Yu, Yeisoo Haberer, Georg Mayer, Klaus F. X. Marri, Pradeep Reddy Rounsley, Steve Chen, Mingsheng Zuccolo, Andrea Panaud, Olivier Wing, Rod A. Roffler, Stefan DNA (class 2) transposons are mobile genetic elements which move within their ‘host’ genome through excising and re-inserting elsewhere. Although the rice genome contains tens of thousands of such elements, their actual role in evolution is still unclear. Analysing over 650 transposon polymorphisms in the rice species Oryza sativa and Oryza glaberrima, we find that DNA repair following transposon excisions is associated with an increased number of mutations in the sequences neighbouring the transposon. Indeed, the 3,000 bp flanking the excised transposons can contain over 10 times more mutations than the genome-wide average. Since DNA transposons preferably insert near genes, this is correlated with increases in mutation rates in coding sequences and regulatory regions. Most importantly, we find this phenomenon also in maize, wheat and barley. Thus, these findings suggest that DNA transposon activity is a major evolutionary force in grasses which provide the basis of most food consumed by humankind. 2016-09-07 2024-12-19T12:54:49Z 2024-12-19T12:54:49Z Journal Article https://hdl.handle.net/10568/165214 en Open Access Springer Wicker, Thomas; Yu, Yeisoo; Haberer, Georg; Mayer, Klaus F. X.; Marri, Pradeep Reddy; Rounsley, Steve; Chen, Mingsheng; Zuccolo, Andrea; Panaud, Olivier; Wing, Rod A. and Roffler, Stefan. 2016. DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses. Nat Commun, Volume 7, no. 1
spellingShingle Wicker, Thomas
Yu, Yeisoo
Haberer, Georg
Mayer, Klaus F. X.
Marri, Pradeep Reddy
Rounsley, Steve
Chen, Mingsheng
Zuccolo, Andrea
Panaud, Olivier
Wing, Rod A.
Roffler, Stefan
DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title_full DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title_fullStr DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title_full_unstemmed DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title_short DNA transposon activity is associated with increased mutation rates in genes of rice and other grasses
title_sort dna transposon activity is associated with increased mutation rates in genes of rice and other grasses
url https://hdl.handle.net/10568/165214
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