Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes
As a first line of defense against insect herbivores many plants store high concentrations of toxic and deterrent secondary metabolites in glandular trichomes. Plant Pleiotropic Drug Resistance (PDR)‐type ABC transporters are known secondary metabolite transporters, and several have been implicated...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2016
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| Acceso en línea: | https://hdl.handle.net/10568/165190 |
| _version_ | 1855534987584995328 |
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| author | Sasse, Joëlle Schlegel, Markus Borghi, Lorenzo Ullrich, Friederike Lee, Miyoung Liu, Guo-Wei Giner, José-Luis Kayser, Oliver Bigler, Laurent Martinoia, Enrico Kretzschmar, Tobias |
| author_browse | Bigler, Laurent Borghi, Lorenzo Giner, José-Luis Kayser, Oliver Kretzschmar, Tobias Lee, Miyoung Liu, Guo-Wei Martinoia, Enrico Sasse, Joëlle Schlegel, Markus Ullrich, Friederike |
| author_facet | Sasse, Joëlle Schlegel, Markus Borghi, Lorenzo Ullrich, Friederike Lee, Miyoung Liu, Guo-Wei Giner, José-Luis Kayser, Oliver Bigler, Laurent Martinoia, Enrico Kretzschmar, Tobias |
| author_sort | Sasse, Joëlle |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | As a first line of defense against insect herbivores many plants store high concentrations of toxic and deterrent secondary metabolites in glandular trichomes. Plant Pleiotropic Drug Resistance (PDR)‐type ABC transporters are known secondary metabolite transporters, and several have been implicated in pathogen or herbivore defense. Here, we report on Petunia hybrida PhPDR2 as a major contributor to trichome‐related chemical defense. PhPDR2 was found to localize to the plasma membrane and be predominantly expressed in multicellular glandular trichomes of leaves and stems. Down‐regulation of PhPDR2 via RNA interference (pdr2) resulted in a markedly higher susceptibility of the transgenic plants to the generalist foliage feeder Spodoptera littoralis. Untargeted screening of pdr2 trichome metabolite contents showed a significant decrease in petuniasterone and petuniolide content, compounds, which had previously been shown to act as potent toxins against various insects. Our findings suggest that PhPDR2 plays a leading role in controlling petuniasterone levels in leaves and trichomes of petunia, thus contributing to herbivory resistance. |
| format | Journal Article |
| id | CGSpace165190 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1651902025-05-14T10:24:21Z Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes Sasse, Joëlle Schlegel, Markus Borghi, Lorenzo Ullrich, Friederike Lee, Miyoung Liu, Guo-Wei Giner, José-Luis Kayser, Oliver Bigler, Laurent Martinoia, Enrico Kretzschmar, Tobias As a first line of defense against insect herbivores many plants store high concentrations of toxic and deterrent secondary metabolites in glandular trichomes. Plant Pleiotropic Drug Resistance (PDR)‐type ABC transporters are known secondary metabolite transporters, and several have been implicated in pathogen or herbivore defense. Here, we report on Petunia hybrida PhPDR2 as a major contributor to trichome‐related chemical defense. PhPDR2 was found to localize to the plasma membrane and be predominantly expressed in multicellular glandular trichomes of leaves and stems. Down‐regulation of PhPDR2 via RNA interference (pdr2) resulted in a markedly higher susceptibility of the transgenic plants to the generalist foliage feeder Spodoptera littoralis. Untargeted screening of pdr2 trichome metabolite contents showed a significant decrease in petuniasterone and petuniolide content, compounds, which had previously been shown to act as potent toxins against various insects. Our findings suggest that PhPDR2 plays a leading role in controlling petuniasterone levels in leaves and trichomes of petunia, thus contributing to herbivory resistance. 2016-12 2024-12-19T12:54:49Z 2024-12-19T12:54:49Z Journal Article https://hdl.handle.net/10568/165190 en Wiley Sasse, Joëlle; Schlegel, Markus; Borghi, Lorenzo; Ullrich, Friederike; Lee, Miyoung; Liu, Guo‐Wei; Giner, José‐Luis; Kayser, Oliver; Bigler, Laurent; Martinoia, Enrico and Kretzschmar, Tobias. 2016. Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes. Plant Cell and Environment, Volume 39 no. 12 p. 2725-2739 |
| spellingShingle | Sasse, Joëlle Schlegel, Markus Borghi, Lorenzo Ullrich, Friederike Lee, Miyoung Liu, Guo-Wei Giner, José-Luis Kayser, Oliver Bigler, Laurent Martinoia, Enrico Kretzschmar, Tobias Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title | Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title_full | Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title_fullStr | Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title_full_unstemmed | Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title_short | Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| title_sort | petunia hybrida pdr2 is involved in herbivore defense by controlling steroidal contents in trichomes |
| url | https://hdl.handle.net/10568/165190 |
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