OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice
Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2017
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| Acceso en línea: | https://hdl.handle.net/10568/165181 |
| _version_ | 1855522506980458496 |
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| author | Li, Jinjie Li, Yang Yin, Zhigang Jiang, Jihong Zhang, Minghui Guo, Xiao Ye, Zhujia Zhao, Yan Xiong, Haiyan Zhang, Zhanying Shao, Yujie Jiang, Conghui Zhang, Hongliang An, Gynheung Paek, Nam-Chon Ali, Jauhar Li, Zichao |
| author_browse | Ali, Jauhar An, Gynheung Guo, Xiao Jiang, Conghui Jiang, Jihong Li, Jinjie Li, Yang Li, Zichao Paek, Nam-Chon Shao, Yujie Xiong, Haiyan Ye, Zhujia Yin, Zhigang Zhang, Hongliang Zhang, Minghui Zhang, Zhanying Zhao, Yan |
| author_facet | Li, Jinjie Li, Yang Yin, Zhigang Jiang, Jihong Zhang, Minghui Guo, Xiao Ye, Zhujia Zhao, Yan Xiong, Haiyan Zhang, Zhanying Shao, Yujie Jiang, Conghui Zhang, Hongliang An, Gynheung Paek, Nam-Chon Ali, Jauhar Li, Zichao |
| author_sort | Li, Jinjie |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation. |
| format | Journal Article |
| id | CGSpace165181 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1651812025-05-14T10:24:02Z OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice Li, Jinjie Li, Yang Yin, Zhigang Jiang, Jihong Zhang, Minghui Guo, Xiao Ye, Zhujia Zhao, Yan Xiong, Haiyan Zhang, Zhanying Shao, Yujie Jiang, Conghui Zhang, Hongliang An, Gynheung Paek, Nam-Chon Ali, Jauhar Li, Zichao Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation. 2017-02 2024-12-19T12:54:48Z 2024-12-19T12:54:48Z Journal Article https://hdl.handle.net/10568/165181 en Open Access Wiley Li, Jinjie; Li, Yang; Yin, Zhigang; Jiang, Jihong; Zhang, Minghui; Guo, Xiao; Ye, Zhujia; Zhao, Yan; Xiong, Haiyan; Zhang, Zhanying; Shao, Yujie; Jiang, Conghui; Zhang, Hongliang; An, Gynheung; Paek, Nam‐Chon; Ali, Jauhar and Li, Zichao. 2017. OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice. Plant Biotechnology Journal, Volume 15 no. 2 p. 183-196 |
| spellingShingle | Li, Jinjie Li, Yang Yin, Zhigang Jiang, Jihong Zhang, Minghui Guo, Xiao Ye, Zhujia Zhao, Yan Xiong, Haiyan Zhang, Zhanying Shao, Yujie Jiang, Conghui Zhang, Hongliang An, Gynheung Paek, Nam-Chon Ali, Jauhar Li, Zichao OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title | OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title_full | OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title_fullStr | OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title_full_unstemmed | OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title_short | OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signaling in rice |
| title_sort | osasr5 enhances drought tolerance through a stomatal closure pathway associated with aba and h2o2 signaling in rice |
| url | https://hdl.handle.net/10568/165181 |
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