The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus
“Fairy rings” resulting from fungus-stimulated plant growth occur all over the world. In 2010, 2-azahypoxanthine (AHX) from a fungus Lepista sordida was identified as the “fairy” that stimulates plant growth. Furthermore, 2-aza-8-oxohypoxanthine (AOH) was isolated as a common metabolite of AHX in pl...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2016
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| Acceso en línea: | https://hdl.handle.net/10568/165168 |
| _version_ | 1855532708514496512 |
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| author | Suzuki, Tomohiro Yamamoto, Naoki Choi, Jae-Hoon Takano, Tomoyuki Sasaki, Yohei Terashima, Yurika Ito, Akinobu Dohra, Hideo Hirai, Hirofumi Nakamura, Yukino Yano, Kentaro Kawagishi, Hirokazu |
| author_browse | Choi, Jae-Hoon Dohra, Hideo Hirai, Hirofumi Ito, Akinobu Kawagishi, Hirokazu Nakamura, Yukino Sasaki, Yohei Suzuki, Tomohiro Takano, Tomoyuki Terashima, Yurika Yamamoto, Naoki Yano, Kentaro |
| author_facet | Suzuki, Tomohiro Yamamoto, Naoki Choi, Jae-Hoon Takano, Tomoyuki Sasaki, Yohei Terashima, Yurika Ito, Akinobu Dohra, Hideo Hirai, Hirofumi Nakamura, Yukino Yano, Kentaro Kawagishi, Hirokazu |
| author_sort | Suzuki, Tomohiro |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | “Fairy rings” resulting from fungus-stimulated plant growth occur all over the world. In 2010, 2-azahypoxanthine (AHX) from a fungus Lepista sordida was identified as the “fairy” that stimulates plant growth. Furthermore, 2-aza-8-oxohypoxanthine (AOH) was isolated as a common metabolite of AHX in plants, and the endogenous existence of AHX and AOH in plants was proved. The structure of AHX allowed us to hypothesize that AHX was derived from 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). Thus, we performed a feeding experiment that supplied AICAR to L. sordida. Consumption of AICAR and accumulation of AHX were observed after feeding. The mycelia extract had enzymatic activity of adenine/5-aminoimidazole-4-carboxamide phosphoribosyltransferase (APRT). APRT gene of L. sordida revealed its structural characteristics in homology modeling and showed transcriptional enhancement after feeding. These results support that AHX was synthesized from AICAR and AHX biosynthesis was transcriptionally controlled by AICAR, indicating the presence of novel purine metabolic pathway in L. sordida. |
| format | Journal Article |
| id | CGSpace165168 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1651682024-12-19T14:13:06Z The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus Suzuki, Tomohiro Yamamoto, Naoki Choi, Jae-Hoon Takano, Tomoyuki Sasaki, Yohei Terashima, Yurika Ito, Akinobu Dohra, Hideo Hirai, Hirofumi Nakamura, Yukino Yano, Kentaro Kawagishi, Hirokazu “Fairy rings” resulting from fungus-stimulated plant growth occur all over the world. In 2010, 2-azahypoxanthine (AHX) from a fungus Lepista sordida was identified as the “fairy” that stimulates plant growth. Furthermore, 2-aza-8-oxohypoxanthine (AOH) was isolated as a common metabolite of AHX in plants, and the endogenous existence of AHX and AOH in plants was proved. The structure of AHX allowed us to hypothesize that AHX was derived from 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). Thus, we performed a feeding experiment that supplied AICAR to L. sordida. Consumption of AICAR and accumulation of AHX were observed after feeding. The mycelia extract had enzymatic activity of adenine/5-aminoimidazole-4-carboxamide phosphoribosyltransferase (APRT). APRT gene of L. sordida revealed its structural characteristics in homology modeling and showed transcriptional enhancement after feeding. These results support that AHX was synthesized from AICAR and AHX biosynthesis was transcriptionally controlled by AICAR, indicating the presence of novel purine metabolic pathway in L. sordida. 2016-12-19 2024-12-19T12:54:46Z 2024-12-19T12:54:46Z Journal Article https://hdl.handle.net/10568/165168 en Open Access Springer Suzuki, Tomohiro; Yamamoto, Naoki; Choi, Jae-Hoon; Takano, Tomoyuki; Sasaki, Yohei; Terashima, Yurika; Ito, Akinobu; Dohra, Hideo; Hirai, Hirofumi; Nakamura, Yukino; Yano, Kentaro and Kawagishi, Hirokazu. 2016. The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus. Sci Rep, Volume 6, no. 1 |
| spellingShingle | Suzuki, Tomohiro Yamamoto, Naoki Choi, Jae-Hoon Takano, Tomoyuki Sasaki, Yohei Terashima, Yurika Ito, Akinobu Dohra, Hideo Hirai, Hirofumi Nakamura, Yukino Yano, Kentaro Kawagishi, Hirokazu The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title | The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title_full | The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title_fullStr | The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title_full_unstemmed | The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title_short | The biosynthetic pathway of 2-azahypoxanthine in fairy-ring forming fungus |
| title_sort | biosynthetic pathway of 2 azahypoxanthine in fairy ring forming fungus |
| url | https://hdl.handle.net/10568/165168 |
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