Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm
Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. The development and use of disease-resistant cultivars have been the most effective strategy to control bacterial blight. Identifying the genes mediating bacterial blight...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2017
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| Acceso en línea: | https://hdl.handle.net/10568/165074 |
| _version_ | 1855517449785442304 |
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| author | Zhang, Fan Wu, Zhi-Chao Wang, Ming-Ming Zhang, Fan Dingkuhn, Michael Xu, Jian-Long Zhou, Yong-Li Li, Zhi-Kang |
| author_browse | Dingkuhn, Michael Li, Zhi-Kang Wang, Ming-Ming Wu, Zhi-Chao Xu, Jian-Long Zhang, Fan Zhou, Yong-Li |
| author_facet | Zhang, Fan Wu, Zhi-Chao Wang, Ming-Ming Zhang, Fan Dingkuhn, Michael Xu, Jian-Long Zhou, Yong-Li Li, Zhi-Kang |
| author_sort | Zhang, Fan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. The development and use of disease-resistant cultivars have been the most effective strategy to control bacterial blight. Identifying the genes mediating bacterial blight resistance is a prerequisite for breeding cultivars with broad-spectrum and durable resistance. We herein describe a genome-wide association study involving 172 diverseOryza sativa ssp. indica accessions to identify loci influencing the resistance to representative strains of six Xoo races. Twelve resistance loci containing 121 significantly associated signals were identified using 317,894 single nucleotide polymorphisms, which explained 13.3–59.9% of the variability in lesion length caused by Xoo races P1, P6, and P9a. Two hotspot regions (L11 and L12) were located within or nearby two cloned R genes (xa25 and Xa26) and one fine-mapped R gene (Xa4). Our results confirmed the relatively high resolution of genome-wide association studies. Moreover, we detected novel significant associations on chromosomes 2, 3, and 6–10. Haplotype analyses of xa25, the Xa26 paralog (MRKc; LOC_Os11g47290), and aXa4 candidate gene (LOC_11g46870) revealed differences in bacterial blight resistance amongindica subgroups. These differences were responsible for the observed variations in lesion lengths resulting from infections by Xoo races P1 and P9a. Our findings may be relevant for future studies involving bacterial blight resistance gene cloning, and provide insights into the genetic basis for bacterial blight resistance in indica rice, which may be useful for knowledge-based crop improvement |
| format | Journal Article |
| id | CGSpace165074 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1650742026-01-06T12:03:44Z Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm Zhang, Fan Wu, Zhi-Chao Wang, Ming-Ming Zhang, Fan Dingkuhn, Michael Xu, Jian-Long Zhou, Yong-Li Li, Zhi-Kang Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. The development and use of disease-resistant cultivars have been the most effective strategy to control bacterial blight. Identifying the genes mediating bacterial blight resistance is a prerequisite for breeding cultivars with broad-spectrum and durable resistance. We herein describe a genome-wide association study involving 172 diverseOryza sativa ssp. indica accessions to identify loci influencing the resistance to representative strains of six Xoo races. Twelve resistance loci containing 121 significantly associated signals were identified using 317,894 single nucleotide polymorphisms, which explained 13.3–59.9% of the variability in lesion length caused by Xoo races P1, P6, and P9a. Two hotspot regions (L11 and L12) were located within or nearby two cloned R genes (xa25 and Xa26) and one fine-mapped R gene (Xa4). Our results confirmed the relatively high resolution of genome-wide association studies. Moreover, we detected novel significant associations on chromosomes 2, 3, and 6–10. Haplotype analyses of xa25, the Xa26 paralog (MRKc; LOC_Os11g47290), and aXa4 candidate gene (LOC_11g46870) revealed differences in bacterial blight resistance amongindica subgroups. These differences were responsible for the observed variations in lesion lengths resulting from infections by Xoo races P1 and P9a. Our findings may be relevant for future studies involving bacterial blight resistance gene cloning, and provide insights into the genetic basis for bacterial blight resistance in indica rice, which may be useful for knowledge-based crop improvement 2017-03-29 2024-12-19T12:54:39Z 2024-12-19T12:54:39Z Journal Article https://hdl.handle.net/10568/165074 en Open Access Public Library of Science Zhang, Fan; Wu, Zhi-Chao; Wang, Ming-Ming; Zhang, Fan; Dingkuhn, Michael; Xu, Jian-Long; Zhou, Yong-Li and Li, Zhi-Kang. 2017. Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm. PLoS ONE, Volume 12 no. 3 p. e0174598 |
| spellingShingle | Zhang, Fan Wu, Zhi-Chao Wang, Ming-Ming Zhang, Fan Dingkuhn, Michael Xu, Jian-Long Zhou, Yong-Li Li, Zhi-Kang Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title | Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title_full | Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title_fullStr | Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title_full_unstemmed | Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title_short | Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| title_sort | genome wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm |
| url | https://hdl.handle.net/10568/165074 |
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