Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.)
# Background The identification and introgression of major-effect QTLs for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. In the present study, we characterized Malaysian rice germplasm for yield and yield-related tra...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2017
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| Online Access: | https://hdl.handle.net/10568/165052 |
| _version_ | 1855526980394418176 |
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| author | Swamy, B.P.Mallikarjuna Shamsudin, Noraziyah Abd Aziz Rahman, Site Noorzuraini Abd Mauleon, Ramil Ratnam, Wickneswari Sta. Cruz, Ma.Teressa Kumar, Arvind |
| author_browse | Kumar, Arvind Mauleon, Ramil Rahman, Site Noorzuraini Abd Ratnam, Wickneswari Shamsudin, Noraziyah Abd Aziz Sta. Cruz, Ma.Teressa Swamy, B.P.Mallikarjuna |
| author_facet | Swamy, B.P.Mallikarjuna Shamsudin, Noraziyah Abd Aziz Rahman, Site Noorzuraini Abd Mauleon, Ramil Ratnam, Wickneswari Sta. Cruz, Ma.Teressa Kumar, Arvind |
| author_sort | Swamy, B.P.Mallikarjuna |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | # Background
The identification and introgression of major-effect QTLs for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. In the present study, we characterized Malaysian rice germplasm for yield and yield-related traits and identified significant trait marker associations by structured association mapping.
# Results
The drought screening was successful in screening germplasm with a yield reduction of up to 60% and heritability for grain yield under drought was up to 78%. There was a wider phenotypic and molecular diversity within the panel, indicating the suitability of the population for quantitative trait loci (QTL) mapping. Structure analyses clearly grouped the accessions into three subgroups with admixtures. Linkage disequilibrium (LD) analysis revealed that LD decreased with an increase in distance between marker pairs and the LD decay varied from 5–20 cM. The Mixed Linear model-based structured association mapping identified 80 marker trait associations (MTA) for grain yield (GY), plant height (PH) and days to flowering (DTF). Seven MTA were identified for GY under drought stress, four of these MTA were consistently identified in at least two of the three analyses. Most of these MTA identified were on chromosomes 2, 5, 10, 11 and 12, and their phenotypic variance (PV) varied from 5% to 19%. The in silico analysis of drought QTL regions revealed the association of several drought-responsive genes conferring drought tolerance. The major-effect QTLs are useful in marker-assisted QTL pyramiding to improve drought tolerance.
# Conclusion
The results have clearly shown that structured association mapping is one of the feasible options to identify major-effect QTLs for drought tolerance-related traits in rice. |
| format | Journal Article |
| id | CGSpace165052 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1650522026-01-06T12:03:48Z Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) Swamy, B.P.Mallikarjuna Shamsudin, Noraziyah Abd Aziz Rahman, Site Noorzuraini Abd Mauleon, Ramil Ratnam, Wickneswari Sta. Cruz, Ma.Teressa Kumar, Arvind # Background The identification and introgression of major-effect QTLs for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. In the present study, we characterized Malaysian rice germplasm for yield and yield-related traits and identified significant trait marker associations by structured association mapping. # Results The drought screening was successful in screening germplasm with a yield reduction of up to 60% and heritability for grain yield under drought was up to 78%. There was a wider phenotypic and molecular diversity within the panel, indicating the suitability of the population for quantitative trait loci (QTL) mapping. Structure analyses clearly grouped the accessions into three subgroups with admixtures. Linkage disequilibrium (LD) analysis revealed that LD decreased with an increase in distance between marker pairs and the LD decay varied from 5–20 cM. The Mixed Linear model-based structured association mapping identified 80 marker trait associations (MTA) for grain yield (GY), plant height (PH) and days to flowering (DTF). Seven MTA were identified for GY under drought stress, four of these MTA were consistently identified in at least two of the three analyses. Most of these MTA identified were on chromosomes 2, 5, 10, 11 and 12, and their phenotypic variance (PV) varied from 5% to 19%. The in silico analysis of drought QTL regions revealed the association of several drought-responsive genes conferring drought tolerance. The major-effect QTLs are useful in marker-assisted QTL pyramiding to improve drought tolerance. # Conclusion The results have clearly shown that structured association mapping is one of the feasible options to identify major-effect QTLs for drought tolerance-related traits in rice. 2017-12 2024-12-19T12:54:38Z 2024-12-19T12:54:38Z Journal Article https://hdl.handle.net/10568/165052 en Open Access Springer Shamsudin, Noraziyah Abd Aziz; Rahman, Site Noorzuraini Abd; Mauleon, Ramil; Ratnam, Wickneswari; Sta. Cruz, Ma. Teressa and Kumar, Arvind. 2017. Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.). Rice, volume 10, no. 21; 13 pages, ill. Ref. |
| spellingShingle | Swamy, B.P.Mallikarjuna Shamsudin, Noraziyah Abd Aziz Rahman, Site Noorzuraini Abd Mauleon, Ramil Ratnam, Wickneswari Sta. Cruz, Ma.Teressa Kumar, Arvind Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title | Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title_full | Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title_fullStr | Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title_full_unstemmed | Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title_short | Association mapping of yield and yield-related traits under reproductive stage drought stress in rice (Oryza sativa L.) |
| title_sort | association mapping of yield and yield related traits under reproductive stage drought stress in rice oryza sativa l |
| url | https://hdl.handle.net/10568/165052 |
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