Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea

In soil, plant roots coexist with bacteria and fungi that produce siderophores capable of sequestering the available iron. Microbial cyanogenesis has been demonstrated in many species of fungi and in a few species of bacteria (e.g., Chromobacterium and Pseudomonas). Fluorescent Pseudomonas isolates...

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Autores principales: Kotasthane, Anil S., Agrawal, Toshy, Zaidi, Najam Waris, Singh, U.S.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2017
Acceso en línea:https://hdl.handle.net/10568/165038
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author Kotasthane, Anil S.
Agrawal, Toshy
Zaidi, Najam Waris
Singh, U.S.
author_browse Agrawal, Toshy
Kotasthane, Anil S.
Singh, U.S.
Zaidi, Najam Waris
author_facet Kotasthane, Anil S.
Agrawal, Toshy
Zaidi, Najam Waris
Singh, U.S.
author_sort Kotasthane, Anil S.
collection Repository of Agricultural Research Outputs (CGSpace)
description In soil, plant roots coexist with bacteria and fungi that produce siderophores capable of sequestering the available iron. Microbial cyanogenesis has been demonstrated in many species of fungi and in a few species of bacteria (e.g., Chromobacterium and Pseudomonas). Fluorescent Pseudomonas isolates P29, P59, P144, P166, P174, P187, P191 and P192 were cyanogenic and produced siderophores in the presence of a strong chelater 8-Hydroxyquinoline (50 mg/l). A simple confrontation assay for identifying potential antagonists was developed. Fluorescent Pseudomonas isolates P66, P141, P144, P166 and P174 were antagonistic against both Rhizoctonia solani and Sclerotium rolfsii. Vigorous plant growth was observed following seed bacterization with P141, P200 and P240. In field experiments, seed bacterization with selected bacterial isolates resulted in reduced collar rot (S. rolfsii) incidence.
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spelling CGSpace1650382025-05-14T10:23:56Z Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea Kotasthane, Anil S. Agrawal, Toshy Zaidi, Najam Waris Singh, U.S. In soil, plant roots coexist with bacteria and fungi that produce siderophores capable of sequestering the available iron. Microbial cyanogenesis has been demonstrated in many species of fungi and in a few species of bacteria (e.g., Chromobacterium and Pseudomonas). Fluorescent Pseudomonas isolates P29, P59, P144, P166, P174, P187, P191 and P192 were cyanogenic and produced siderophores in the presence of a strong chelater 8-Hydroxyquinoline (50 mg/l). A simple confrontation assay for identifying potential antagonists was developed. Fluorescent Pseudomonas isolates P66, P141, P144, P166 and P174 were antagonistic against both Rhizoctonia solani and Sclerotium rolfsii. Vigorous plant growth was observed following seed bacterization with P141, P200 and P240. In field experiments, seed bacterization with selected bacterial isolates resulted in reduced collar rot (S. rolfsii) incidence. 2017-06 2024-12-19T12:54:38Z 2024-12-19T12:54:38Z Journal Article https://hdl.handle.net/10568/165038 en Open Access Springer Kotasthane, Anil S.; Agrawal, Toshy; Zaidi, Najam Waris and Singh, U. S. 2017. Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea. 3 Biotech, Volume 7, no. 2
spellingShingle Kotasthane, Anil S.
Agrawal, Toshy
Zaidi, Najam Waris
Singh, U.S.
Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title_full Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title_fullStr Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title_full_unstemmed Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title_short Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea
title_sort identification of siderophore producing and cynogenic fluorescent pseudomonas and a simple confrontation assay to identify potential bio control agent for collar rot of chickpea
url https://hdl.handle.net/10568/165038
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